Research(if you don't see a menu on the left, use this
link) Using Crystallography, and
therefore bringing
together Physics, Chemistry, Engineering and Robotics, we investigate
the form and function of biological molecules, as well as their
complexes, in order to understand the function of biological systems. The main objective of the
CBE Department is to interpret
biological phenomena in terms of structural studies at the atomic,
molecular and supramolecular level, and to use this knowledge
to solve biomedical and biotechnological problems.
The research of the Department is focused in the following lines:
Bacterial immunity We study the arms race between
bacteria and their phage foes, focusing on the molecular basis of
prokaryotic anti-viral defence systems and viral countermeasures. To
address it, we use a combination of bioinformatics, molecular biology,
biochemistry and structural biology techniques. Understanding the
dynamics of the interactions between these microorganisms has profound
implications for phage-based therapies and the development of new
biotechnological tools.
Uncovering
protein
structure and function with XFELs
Our
research group employs the state-of-the-art technology of serial
femtosecond crystallography at X-ray Free Electron Lasers to study
structural dynamics of biological macromolecules in "real time" to
understand how they function. With this cutting-edge technique we aim
to solve pressing problems of current biomedicine and biotechnology
such as antibiotic resistance and cancer to ultimately develop more
effective and lower cost treatments. Infectious disease and drug
discovery We
are interested in the structural and functional study of proteins
involved in host-pathogen interactions from pathogenic mycobacteria and
fungi. To do so, we use a multi-disciplinary approach combining
molecular biology, protein engineering, biophysical and structural
biology techniques. Moreover, we aim at using this information for the
rational design of molecules with antimicrobial activity.
Regulation of
protein-protein interactions with therapeutic potential in neuronal
disease
Our research is focused in understanding at molecular level Ca2+
mediated protein-protein interactions (PPI) that are key in neuronal
function and nervous system development. We carry out a
multidisciplinary approach using strategies of Structural, Biochemical,
Biophysical, Molecular and Chemical Biology to find out PPI small
molecule regulators that may constitute potential therapeutic compounds
in neuronal diseases.
Multicomponent formation
Methods
to predict the probabilities for Active Pharmaceutical Ingredients
(API) to crystallize with a coformer as a way to improve their
physicochemical properties. Important to reduce drug doses and
therefore side effects.
Inositide signaling Inositol polyphosphates (IP) are
pleiotropic molecules critical for metabolism and signaling. Our
research is focused on the Structural Biology of proteins that act as
IP targets and particularly on the IP-target association in order to
understand IP regulation and target functioning. IPs wrong regulation
underlie severe pathologies as cancer. Therefore an in-depth
characterization at molecular level of their targets will facilitate
drug development against principal diseases.
Structural Microbiology We
focus on the structural biology, combined with biophysical and
bioinformatics tools, of the bacterial surface proteins involved in key
functions, such as cell-wall remodeling, host-pathogen interactions,
antibiotics resistance, cell division, oxidative stress resistance, or
pathogenesis mechanisms. The main goal is to study the mechanisms of
bacterial virulence and pathogenesis and the means of circumvent them. Plant Structural Biology
We investigate the molecular basis of plant response to abiotic stress
as we KNOW this knowledge can be used to improve crop yields. We
BELIEVE that this can help alleviate the consequences of global warming
and feed the growing world population.
Structural
Biotechnology We
disclose the structural and molecular basis of the catalytic mechanisms
of enzymes, as a platform to design highly efficient biocatalysts with
novel functionalities for biotechnology and synthetic biology. Current
research focus on multidomain enzymes and multienzyme cascades to be
used as catalytic nanomachines.
Gut microbial enzymes Human microbiome exerts a
profound influence on health and disease. We are investigating how gut
microbial enzymes affect host biology and in particular their link to
promotion or development of human disease. We believe that
characterization of these relations will pave the way to new
therapeutic strategies targeting microbiomes rather than the human
host.
Publications 2023 Regulation of the Cell Division Hydrolase RipC by the FtsEX system in Mycobacterium tuberculosis J. Li, X. Xu, W-Z Chua, J. Shi, J.A. Hermoso, L-T Sham, M. Luo Nature Communications (2023) 14:7999(doi: 10.1038/s41467-023-43770-6)
The neuronal calcium
sensor NCS-1 regulates the phosphorylation state and activity of the Gα
chaperone and GEF Ric-8A
Daniel Muñoz-Reyes, Levi J McClelland, Sandra Arroyo-Urea,
Sonia
Sánchez-Yepes, Juan Sabín, Sara
Pérez-Suárez, Margarita Menendez, Alicia
Mansilla, Javier
García-Nafría, Stephen Sprang, Maria
José
Sánchez-Barrena eLife (2023) 12:e86151
(doi: 10.7554/eLife.86151) bioRxiv2022.12.09.519724 (2022)
(doi: 10.1101/2022.12.09.519724)
Structural characterization of PaaX, the main repressor of the
phenylacetate degradation pathway in Escherichia coli W:
a novel fold of transcription regulator proteins
Hernández-Rocamora V.M., Molina R., Alba A.,
Carrasco-López C., Rojas-Altuve A., Panjikar S., Medina A.,
Uson
I., Alfonso C., Galán B., Rivas G., Hermoso J.A. &
Sanz J.M.
International Journal of
Biological Macromolecules (2023) 254, 127935 (doi:
10.1016/j.ijbiomac.2023.127935)
Heterogeneity in M.
tuberculosis β-lactamase inhibition by Sulbactam Malla
TN, Zielinski K, Aldama L, Bajt S, Feliz D, Hayes B, Hunter M, Kupitz
C, Lisova S, Knoska J, Martin-Garcia JM, Mariani V, Pandey S, Poudyal
I, Sierra RG, Tolstikova A, Yefanov O, Yoon CH, Ourmazd A, Fromme P,
Schwander P, Barty A, Chapman HN, Stojkovic EA, Batyuk A, Boutet S,
Phillips GN Jr, Pollack L, Schmidt M Nature Communications
(2023) 14(1), 5507 (doi:
10.1038/s41467-023-41246-1)
Catalytic process of anhydro-N-acetylmuramic acid kinase (AnmK) from
Pseudomonas aeruginosa
Amr
M. El-Araby, Eva Jiménez-Faraco, Rhona Feltzer, Jose M.
Martin-Garcia,
Bhaskara Rao Karri, Balajee Ramachandran, Choon Kim, Jed F. Fisher,
Juan A. Hermoso, Shahriar Mobashery Journal of Biological Chemistry (2023) (doi:
10.1016/j.jbc.2023.105198)
DipM controls multiple autolysins and mediates a regulatory feedback
loop promoting cell constriction in Caulobacter crescentus Adrian Izquierdo-Martinez, Maria
Billini, Vega
Miguel-Ruano, Rogelio Hernández-Tamayo, Pia Richter, Jacob
Biboy, María T. Batuecas, Timo Glatter, Waldemar Vollmer,
Peter
L. Graumann, Juan A. Hermoso and Martin Thanbichler Nature
Communications (2023) 14, Article number: 4095(doi:
10.1038/s41467-023-39783-w)
Molecular basis of the final step of cell division in Streptococcus
pneumoniae
Siseth Martínez-Caballero, Céline Freton, Rafael
Molina,
Sergio G. Bartual, Virginie Gueguen-Chaignon, Chryslène
Mercy,
Federico Gago, Kiran V. Mahasenan,
Inés G. Muñoz, Mijoon Lee, Dusan Hesek, Shahriar
Mobashery, Juan A. Hermoso, Christophe Grangeasse Cell Reports (2023)
(doi:
10.1016/j.celrep.2023.112756)
Mechanistic insights
into the regulation of cell wall hydrolysis by FtsEX and EnvC at the
bacterial division site Xin Xu, Jianwei Li, Wan-Zhen
Chua, Martin Alcorlo, Jian Shi, Juan A. Hermoso, Thomas G Bernhardt,
Lok-To Sham, Min Luo Proceedings
of the National Academy of Sciences, PNAS(2023)(doi: 10.1073/pnas.2301897120)(see also movie-1
& movie-2) Modular Droplet
Injector for Sample
Conservation Providing New Structural Insight for the Conformational
Heterogeneity in the Disease-Associated NQO1 Enzyme D.
Doppler, M. Sonker, A. Egatz-Gomez, A. Grieco, S. Zaare, R. Jernigan,
J.D. Meza-Aguilar, M.T. Rabbani, A. Manna, R. Alvarez, K. Karpos, J.C.
Villarreal, G. Nelson, J-H Yang, J. Carrion, K. Morin, G.K. Ketawala,
A.L. Pey, M.A. Ruiz-Fresneda, J.L. Pacheco‐Garcia, J.A. Hermoso, R.
Nazari, R. Sierra, M.S. Hunter, A. Batyuk, C.J. Kupitz, R.E. Sublett,
S. Lisova, V. Mariani, S. Boutet, R. Fromme, T.D. Grant, S. Botha, P.
Fromme, R.A. Kirian, J.M. Martin-Garcia*, A. Ros* Lab
on a Chip (2023) 23, 3016–3033(doi: 10.1039/D3LC00176H) Continuous
development in macromolecular
crystallography with CCP4
Martín Martinez-Ripoll and Armando Albert Acta Crystallographica, Section D
(2023) D79, 447-448 (doi:
10.1107/S205979832300445X)(see
also this link) Engineering a Highly Regioselective
Fungal Peroxygenase for the Synthesis of Hydroxy Fatty Acids P
Gomez de Santos, C Aranda, A Fernandez-Garcia, A But, P Molina-Espeja,
D Mate Maté, D Gonzalez-Perez, W Zhang, J Kiebist, K
Scheibner, M
Hofrichter, J Sanz-Aparicio, F Hollmann, A Gutiérrez, and M
Alcalde Angewandte Chemie (2023) 62,
e202217372 (doi: 10.1002/anie.202217372)
The mobility of the cap
domain is essential for the substrate promiscuity of a family IV
esterase from sorghum rhizosphere microbiome M
Distaso, I Cea-Rama, C Coscolín, T N. Chernikova, H Tran, M
Ferrer, J Sanz-Aparicio, PN Golyshin Applied and Environmental
Microbiology Journal (2023)89 (1),
e01807-22(doi: 10.1128/aem.01807-22)
Transforming an esterase into an
enantioselective catecholase through bioconjugation of a versatile
metal-chelating inhibitor L
Fernandez-Lopez, I Cea-Rama, J Alvarez-Malmagro, AK Ressmann, JL
Gonzalez-Alfonso, C Coscolín, P Shahgaldian, FJ Plou,
JModregger, M
Pita, J Sanz-Aparicio, M Ferrer ChemComm
(2023) 59 (62), 9469-9472(doi: 10.1039/D3CC01946B)
Increased thermostability of an engineered flavin-containing
monooxygenase to remediate trimethylamine in fish protein hydrolysates M
Goris, I Cea-Rama, P Puntervoll, R Ree, D Almendral, J Sanz-Aparicio, M
Ferrer, GE Kjæreng Bjerga Applied and Environmental
Microbiology Journal (2023)89 (6), e00390-23
(doi: 10.1128/aem.00390-23)
Relevance of aromatic and polar amino
acids in the specificity of
Inulinase ISO3 from Kluyveromyces marxianus: A molecular dynamics
approach with an experimental verification J
Trapala, M González-Andrade, C Olvera, M Cayetano, J
Sanz-Aparicio, E Jimenez-Ortega, I Bustos-Jaimes, C Montiel International Journal of
Biological Macromolecules (2023) 243, 124734 (doi: 10.1016/j.ijbiomac.2023.124734)
Discovery of
lipid-mediated protein–protein interactions in living cells
using
metabolic labeling with photoactivatable clickable probes
R.O. Fedoryshchak, A. Gorelik, M. Shen, M.M. Shchepinova, I.
Pérez-Dorado, and E.W. Tate Chemical Science (2023) 14,
2419-2430 (doi:
10.1039/D2SC06116C)
Enzymes for consumer products to achieve climate neutrality
Patricia
Molina-Espeja, Julia Sanz-Aparicio, Peter N Golyshin, Ana
Robles-Martín, Víctor Guallar, Fabrizio
Beltrametti, Markus Müller,
Mikhail Yakimov, Jan Modregger, Moniec van Logchem, Philippe Corvini,
Patrick Shahgaldian, Christian Degering, Susanne Wieland, Anne Timm,
Carla C C R de Carvalho, Ilaria Re, Sara Daniotti, Stephan Thies,
Karl-Erich Jaeger, Jennifer Chow, Wolfgang R Streit, Roland Lottenbach,
Rainer Rösch, Nazanin Ansari, Manuel Ferrer, The FuturEnzyme
Consortium Oxford Open Climate Change (2023)
kgad003, (doi: 10.1093/oxfclm/kgad003)
Structure-guided
engineering of a receptor-agonist pair for inducible activation of the
ABA adaptive response to drought
Jorge Lozano-Juste, Lourdes Infantes, Irene Garcia-Maquilon, Rafael
Ruiz-Partida, Ebe Merilo, Juan Luis Benavente, Adrian Velazquez-Campoy,
Alberto Coego, Mar Bono, Javier Forment, Begoña
Pampín,
Paolo Destito, Adrián Monteiro, Ramón
Rodríguez,
Jacobo Cruces, Pedro L. Rodriguez, and Armando Albert Science
Advances (2023) 9(10)(doi:
10.1126/sciadv.ade9948) (see also
a video)
Engineering a Highly
Regioselective
Fungal Peroxygenase for the Synthesis of Hydroxy Fatty Acids P
Gomez de Santos, C Aranda, A Fernandez-Garcia, A But, P Molina-Espeja,
D Mate Maté, D Gonzalez-Perez, W Zhang, J Kiebist, K
Scheibner, M
Hofrichter, J Sanz-Aparicio, F Hollmann, A Gutiérrez, and M
Alcalde Angewandte Chemie (2023)
(doi: 10.1002/anie.202217372)
Structural basis for cyclosporin isoform-specific inhibition of
cyclophilins from Toxoplasma
gondii Favretto F.,
Jiménez-Faraco E., Conter C., Dominici P., Hermoso J.A. and
Astegno A. ACS
Infectious Diseases (2023) (doi:
10.1021/acsinfecdis.2c00566)
2022
El "Rockefeller" cumple 90 años en la vanguardia de la
investigación científica
Albert, A., Martínez-Ripoll, M. Anales
de Química (2022) 118(4), 273-274(link to
the article)
The inhibition of NCS-1 binding to Ric8a rescues fragile X syndrome
mice model phenotypes
Cogram P, Fernández-Beltrán LC, Casarejos MJ,
Sánchez-Yepes S, Rodríguez-Martín E,
García-Rubia A, Sánchez-Barrena MJ, Gil C,
Martínez A, Mansilla A Frontiers in Neuroscience (2022)
16, 1007531 (doi:
10.3389/fnins.2022.1007531)
Insights
into the Structure of the Highly
Glycosylated Ffase from Rhodotorula
dairenensis Enhance Its Biotechnological Potential E.
Jiménez-Ortega, E. Narmontaite, B.
González-Pérez,
Francisco J. Plou, M Fernández-Lobato, J Sanz-Aparicio International
Journal of
Molecular Sciences (2022) 23, 14981(doi: 10.3390/ijms232314981)
Identification of
1,2,3-triazolium salt-based inhibitors of Leishmania
infantum
trypanothione disulfide reductase with enhanced antileishmanial potency
in cellulo
and increased selectivity
Héctor de Lucio, Alejandro Revuelto, Alejandra A.Carriles,
Sonia
de Castro Sonia García-González, Juan Carlos
García-Soriano, Mercedes
Alcón-Calderón, Pedro
A. Sánchez-Murcia, Juan A.Hermoso, Federico Gago,
María-José Camarasa, Antonio
Jiménez-Ruiz,
Sonsoles Velázquez European Journal of Medicinal
Chemistry (2022) 244, 114878 (doi: 10.1016/j.ejmech.2022.114878)
Verification: model-free phasing with enhanced predicted models in
ARCIMBOLDO_SHREDDER
Ana Medina, Elisabet Jiménez, Iracema Caballero, Albert
Castellví, Josep Triviño Valls, Martin Alcorlo,
Rafael
Molina, Juan A. Hermoso, Massimo D. Sammito, Rafael Borges and Isabel
Usón Acta Crystallographica (2022)
D78, 1-11 (doi: 10.1107/S2059798322009706)
Molecular basis of cyclic
tetra-oligoadenylate processing by small standalone CRISPR-Cas ring
nucleases
Rafael Molina, Ricardo Garcia-Martin, Blanca
López-Méndez, Anne Louise Grøn Jensen,
J Rafael
Ciges-Tomas, Javier Marchena-Hurtado, Stefano Stella, Guillermo Montoya Nucleic Acids Research
(2022)
(doi:
10.1093/nar/gkac923)
Nucleus-translocated
mitochondrial cytochrome c liberates nucleophosmin-sequestered ARF
tumor suppressor by changing nucleolar liquid-liquid phase separation González-Arzola
K., Díaz-Quintana A., Bernardo-García N.,
Martínez-Fábregas J., Rivero-Rodríguez
F.,
Casado-Combreras M.A., Elena-Real C.A., Velázquez-Cruz A.,
Gil-Caballero S., Velázquez-Campoy A., Szulc E.,
Gavilán
M.P., Ayala I., Arranz R., Ríos R.M., Salvatella X.,
Valpuesta
J.M., Hermoso J.A., De la Rosa M.A., Díaz-Moreno I. Nature Structural &
Molecular Biology (2022), 29, 1024–1036
(doi: 10.1038/s41594-022-00842-3)
Regulation of Lytic Machineries by the FtsEX Complex in the Bacterial
Divisome
Martin Alcorlo; Siseth Martinez-Caballero; Rafael Molina; Juan A.
Hermoso
in: Macromolecular
Protein Complexes IV. Subcellular Biochemistry, vol 99. Springer, Cham.
(2022), Eds.:
Harris, J.R., Marles-Wright, J. (doi: 10.1007/978-3-031-00793-4_9)
Structure–function insights
into the fungal endo-chitinase Chit33 depict its mechanism on chitinous
material
E Jiménez-Ortega, PE Kidibule, M
Fernández-Lobato, J Sanz-Aparicio
International Journal of Molecular Sciences (2022) 23, 7599
(doi: 10.3390/ijms23147599)
Crystal structure of a family VIII β-lactamase fold hydrolase
reveals the molecular mechanism for its broad substrate scope
I Cea-Rama, C Coscolín, J L Gonzalez-Alfonso, J Raj, M
Vasiljević, F J Plou, M Ferrer, J Sanz-Aparicio The Febs Journal (2022)
(doi: 10.1111/febs.16554)
Structure-Based
Modulation of the Ligand Sensitivity of a Tomato Dimeric Abscisic Acid
Receptor Through a Glu to Asp Mutation in the Latch Loop
Lourdes
Infantes, Maria Rivera-Moreno, Miguel Daniel-Mozo, Juan Luis Benavente,
Javier Ocaña-Cuesta, Alberto Coego, Jorge Lozano-Juste,
Pedro L.
Rodriguez, and Armando Albert Frontiers in Plant Science (2022)
13: 884029 (doi:
10.3389/fpls.2022.884029)
Structural and biophysical properties of FopA, a major outer membrane
protein of Francisella
tularensis Nagaratnam
N, Martin-Garcia JM, Yang JH, Goode MR, Ketawala G, Craciunescu FM,
Zook JD, Sonowal M, Williams D, Grant TD, Fromme R, Hansen DT, Fromme P PLoS One (2022) 17(8),
e0267370 (doi:
10.1371/journal.pone.0267370)
Purification,
characterization, and preliminary serial crystallography diffraction
advances structure determination of full-length human particulate
guanylyl cyclase A receptor Zhang
S, Hansen DT, Martin-Garcia JM, Zook JD, Pan S, Craciunescu FM, Burnett
JC Jr, Fromme P Scientific
Reports (2022) 12(1),
11824
(doi:
10.1038/s41598-022-15798-z)
PYL1-
and PYL8-like ABA Receptors of Nicotiana
benthamiana Play a Key Role in ABA Response in Seed and
Vegetative Tissue
Gaston
A. Pizzio, Cristian Mayordomo, Jorge Lozano-Juste, Victor
Garcia-Carpintero, Marta Vazquez-Vilar, Sergio G. Nebauer, Kacper P.
Kaminski, Nikolai V. Ivanov, Juan C. Estevez, Maria Rivera-Moreno,
Armando Albert, Diego Orzaez and Pedro L. Rodriguez Cells
(2022) 11, 795 (doi: 10.3390/cells11050795)
Serial
macromolecular crystallography at
ALBA Synchrotron Light Source
J. M.
Martin-Garcia,
S. Botha, H. Hu, R. Jernigan, A. Castellví, S. Lisova, F.
Gil,
B. Calisto, I. Crespo, S. Roy-Chowdhury, A. Grieco, G. Ketawala, U.
Weierstall, J. Spence, P. Fromme, N. Zatsepin, D. R. Boer and X. Carpena Journal of Synchrotron Radiation
(2022) 29 (doi:10.1107/S1600577522002508)
A
structurally unique Fusobacterium
nucleatum tannase provides detoxicant activity against
gallotannins and pathogen resistance Mancheño JM,
Atondo E,
Tomás-Cortázar J, Lavín JL,
Plaza-Vinuesa L,
Martín-Ruiz I, Barriales D, Palacios A, Navo CD, Sampedro L,
Peña-Cearra A, Pascual-Itoiz MA, Castelo J,
Carreras-González A, Castellana D, Pellón A,
Delgado S,
Ruas-Madiedo P, de las Rivas B, Abecia L, Muñoz R,
Jiménez-Osés G, Anguita J y Rodríguez H Microbial
Biotechnology (2022)
15, 648-667 (doi:
10.1111/1751-7915.13732)
Co-flow injection for serial crystallography at X-ray free-electron
lasers
D. Doppler, M. T. Rabbani, R. Letrun, J. Cruz Villarreal, D. H. Kim, S.
Gandhi, A. Egatz-Gomez, M. Sonker, J. Chen, F. H. M. Koua, J. Yang, M.
Youssef, V. Mazalova, S. Bajt, M. L. Shelby, M. A. Coleman, M. O.
Wiedorn, J. Knoska, S. Schön, T. Sato, M. S. Hunter, A.
Hosseinizadeh, C. Kuptiz, R. Nazari, R. C. Alvarez, K. Karpos, S.
Zaare, Z. Dobson, E. Discianno, S. Zhang, J. D. Zook, J. Bielecki, R.
de Wijn, A. R. Round, P. Vagovic, M. Kloos, M. Vakili, G. K. Ketawala,
N. E. Stander, T. L. Olson, K. Morin, J. Mondal, J. Nguyen, J. D.
Meza-Aguilar, G. Kodis, S. Vaiana, J. M. Martin-Garcia,
V. Mariani, P. Schwander, M. Schmidt, M. Messerschmidt, A. Ourmazd, N.
Zatsepin, U. Weierstall, B. D. Bruce, A. P. Mancuso, T. Grant, A.
Barty, H. N. Chapman, M. Frank, R. Fromme, J. C. H. Spence, S. Botha,
P. Fromme, R. A. Kirian and A. Ros Journal of
Applied
Crystallography (2022) 55 (doi:
org/10.1107/S1600576721011079)
2021 The
bone-degrading enzyme machinery: from
multi-component understanding to the treatment of residues from the
meat industry
L Fernandez-Lopez, S Sanchez-Carrillo, A García-Moyano, E
Borchert, D Almendral, S Alonso, I Cea-Rama, N Miguez, Ø
Larsen,
J Wernere, KS Makarova, FJ Plou, TG Dahlgren, J Sanz-Aparicio, U.
Hentschelc, G E Kjæreng and M Ferrer Computational
and Structural Biotechnology Journal (2021) 19,
6328-6342 (doi: 10.1016/j.csbj.2021.11.027)
Structural bases of life and evolution of macromolecular complexity Valpuesta
J.M., Ramón-Maiques S., Carazo J.M., González C.,
Hermoso J.A., Moreno-Herrero F., San Martín C.
In: Origins,
(Co)Evolution, Diversity and Synthesis of Life; P. Bovolenta (2021),
Ed. CSIC, ISBN:
978-84-00-10737-6
Identification of the first structurally validated covalent ligands of
the small GTPase RAB27A
M.
Jamshidiha, T. Layon-Hogg, C.L. Sutherell, G.B. Craven, M. Tersa, E. De
Vita, D. Brustur, I. Pérez-Dorado, S. Hassan, R. Petracca,
R.M. Morgan,
M. Sanz-Hermández, J.C. Norman, Al. Armstrong, D.J. Mann, E.
Cota, and
E.W. Tate RSC Medical
Chemistry (2021)(doi:
10.1039/D1MD00225B)
Benzimidazole
derivatives as new and selective inhibitors of arginase from Leishmania
mexicana with biological activity against promastigotes
and amastigotes Betancourt-Conde
I., Avitia-Domínguez C.,
Hernández-Campos A.,
Castillo R., Yépez-Mulia L., Oria-Hernández J.,
Méndez S.T., Sierra-Campos E., Valdez-Solana M.,
Martínez-Caballero S., Hermoso J.A., Romo-Mancillas A. and
Téllez-Valencia A. International
Journal of Molecular Sciences (2021) 22, 13613(doi: 10.3390/ijms222413613)
Co-flow
injection for serial crystallography at X-ray free-electron lasers
D. Doppler, M. T. Rabbani, R. Letrun, J. Cruz Villarreal, D. H. Kim, S.
Gandhi, A. Egatz-Gomez, M. Sonker, J. Chen, F. H. M. Koua, J. Yang, M.
Youssef, V. Mazalova, S. Bajt, M. L. Shelby, M. A. Coleman, M. O.
Wiedorn, J. Knoska, S. Schön, T. Sato, M. S. Hunter, A.
Hosseinizadeh, C. Kuptiz, R. Nazari, R. C. Alvarez, K. Karpos, S.
Zaare, Z. Dobson, E. Discianno, S. Zhang, J. D. Zook, J. Bielecki, R.
de Wijn, A. R. Round, P. Vagovic, M. Kloos, M. Vakili, G. K. Ketawala,
N. E. Stander, T. L. Olson, K. Morin, J. Mondal, J. Nguyen, J. D.
Meza-Aguilar, G. Kodis, S. Vaiana, J. M. Martin-Garcia,
V. Mariani, P. Schwander, M. Schmidt, M. Messerschmidt, A. Ourmazd, N.
Zatsepin, U. Weierstall, B. D. Bruce, A. P. Mancuso, T. Grant, A.
Barty, H. N. Chapman, M. Frank, R. Fromme, J. C. H. Spence, S. Botha,
P. Fromme, R. A. Kirian and A. Ros Journal of
Applied Crystallography (2022)
55 (doi:
10.1107/S1600576721011079)
Structural
inspection and protein motions
modelling of a fungal
glycoside hydrolase family 18 chitinase by crystallography depicts
dynamic enzymatic mechanism
E Jiménez-Ortega, PE Kidibule, M
Fernández-Lobato, J Sanz-Aparicio Computational
and Structural Biotechnology Journal (2021) 19,
5466-5478(doi:
10.1016/j.csbj.2021.09.027)
First Lanthanide Complex for De
Novo Phasing in Native Protein Crystallography at 1
Å Radiation Prieto-Castañeda,
A., Martínez-Caballero, S., Agarrabeitia, A.,
García-Moreno, I., de la Moya, S., Ortiz, M. and Hermoso
J.A. ACS Applied
Biomaterials (2021)
4, 4575-4581 (doi:
10.1021/acsabm.1c00305)
Teaching an old dog new tricks: Repurposing β-lactams Miguel-Ruano,
V. and Hermoso J.A. Trends in
Pharmacological
Sciences (2021) 42, 617-619(doi:
10.1016/j.tips.2021.06.003)
Biochemical and structural
studies of two
tetrameric nucleoside 2'-deoxyribosyltransferases from psychrophilic
and mesophilic bacteria: Insights into cold-adaptation Fernández-Lucas
J,
Acebrón I, Wu RY, Alfaro Y, Acosta J, Kaminski PA, Arroyo M,
Joachimiak A, Nocek BP, De la Mata I, Mancheño JM International
Journal of
Biological Macromolecules (2021) 192, 138-150(doi:
10.1016/j.ijbiomac.2021.09.164)
Integrative
Structural Biology of the Penicillin-Binding Protein-1 from
Staphylococcus aureus,
an Essential Component of the Divisome Machinery Martínez-Caballero
S., Mahasenan K.V., Kim C.; Molina R., Feltzer R., Lee M.; Bouley R.,
Hesek D., Fisher J.F., Muñoz I.G., Chang M.; Mobashery S.
and Hermoso
J.A. Computational
and Structural Biotechnology Journal (2021) 19, 5392-5405(doi:
10.1016/j.csbj.2021.09.018)
Observation of substrate diffusion and ligand binding in enzyme
crystals using high-repetition-rate mix-and-inject serial
crystallography
Suraj Pandey, George Calvey, Andrea M. Katz, Tek Narsingh Malla, Faisal
H. M. Koua, Jose M. Martin-Garcia, Ishwor Poudyal, Jay-How Yang,
Mohammad Vakili, Oleksandr Yefanov, Kara A. Zielinski, Sasa Bajt, Salah
Awel, Katarina Doerner, Matthias Frank, Luca Gelisio, Rebecca Jernigan,
Henry Kirkwood, Marco Kloos, Jayanath Koliyadu, Valerio Mariani,
Mitchell D. Miller, Grant Mills, Garrett Nelson, Jose L. Olmos Jr,
Alireza Sadri, Tokushi Sato, Alexandra Tolstikova, Weijun Xu, Abbas
Ourmazd, John C. H. Spence, Peter Schwander, Anton Barty, Henry N.
Chapman, Petra Fromme, Adrian P. Mancuso, George N. Phillips Jr,
Richard Bean, Lois Pollack and Marius Schmidt IUCrJ
(2021) 8, 878-895 (doi: 10.1107/S2052252521008125)
It
takes two to tango: Unraveling a new post-translational modification
involved in SnRK2.6 activation
Miguel Daniel-Mozo, Armando Albert Molecular
Plant
(2021) 14, 1–3(doi:
10.1016/j.molp.2021.08.003) The
structure and flexibility analysis of the Arabidopsis synaptotagmin
1 reveal the basis of its regulation at membrane contact sites
Juan
L Benavente, Dritan Siliqi, Lourdes
Infantes, Laura Lagartera,
Alberto Mills, Federico Gago, Noemí
Ruiz-López, Miguel A Botella,
María J Sánchez-Barrena, Armando Albert
Life
Science Alliance (2021) 4, e202101152(doi:
10.26508/lsa.202101152)
Synaptotagmins
at the endoplasmic reticulum-plasma membrane contact sites maintain
diacylglycerol homeostasis during abiotic stress
Ruiz-Lopez N,
Pérez-Sancho J, Esteban Del Valle A, Haslam RP, Vanneste S,
Catalá R,
Perea-Resa C, Van Damme D, García-Hernández S,
Albert A, Vallarino J,
Lin J, Friml J, Macho AP, Salinas J, Rosado A, Napier JA, Amorim-Silva
V, Botella MA. Plant Cell
(2021) 33,
2431–2453 (doi:
10.1093/plcell/koab122)
Pharmacological Approaches for the Modulation of the Potassium Channel
KV4.x and KChIPs
Cercós
P, Peraza DA, Benito-Bueno A, Socuéllamos PG, Aziz-Nignan A,
Arrechaga-Estévez D, Beato E, Peña-Acevedo E,
Albert A, González-Vera
JA, Rodríguez Y, Martín-Martínez M,
Valenzuela C, Gutiérrez-Rodríguez
M. International
Journal of
Molecular Sciences (2021) 22, 1419 (doi:
10.3390/ijms22031419)
Protein Dynamics and Time Resolved Protein Crystallography at
Synchrotron Radiation Sources: Past, Present and Future.
Martin-Garcia, J.M. Crystals
(2021) 11, 521(doi:
10.3390/cryst11050521)
Enzymatic Synthesis of Phloretin α‐Glucosides Using a Sucrose
Phosphorylase Mutant and its Effect on Solubility, Antioxidant
Properties and Skin Absorption
J L Gonzalez‐Alfonso, Z Ubiparip, E Jimenez‐Ortega, A Poveda, C Alonso,
L Coderch, J Jimenez‐Barbero, J Sanz‐Aparicio, A O Ballesteros, T
Desmet, F J Plou Advanced
Synthesis &
Catalysis (2021) 363, 3079-3089(doi:
10.1002/adsc.202100201)
Multiple substrate recognition by yeast diadenosine and
diphosphoinositol polyphosphate phosphohydrolase through phosphate
clamping
María Ángeles
Márquez-Moñino, Raquel
Ortega-García, Megan L. Shipton, Elsa
Franco-Echevarría,
Andrew M. Riley, Julia Sanz-Aparicio, Barry V.L. Potter and Beatriz
González Science Advances (2021)
7, abf6744(doi:
10.1126/sciadv.abf6744)
Phylogenetic, functional and structural characterization of a GH10
xylanase active at extreme conditions of temperature and alkalinity
D Talens-Perales, E Jiménez-Ortega, P
Sánchez-Torres, J Sanz-Aparicio, J Polaina Computational
and Structural Biotechnology Journal (2021) 19, 2676-2686
(doi:
10.1016/j.csbj.2021.05.004)
Drug
resistance in infectious diseases
Gamarro
F., Garcillán M.P., Rivas L., Espinosa M., Khiar N., Hermoso
J.A.,
Garcia T., Martínez, J.L., Boto A, Galla T.,
Blázquez J. Gonzalez de la
Campa A.
in Challenges
in Biomedicine and Health (2021), M. Delgado Ed. CSIC, pp.
64-88 . ISBN:978-84-00-10744-4
New insights into the molecular mechanism behind mannitol and
erythritol fructosylation by β-fructofuranosidase from
Schwanniomyces occidentalis
D Rodrigo-Frutos, M Ramirez-Escudero, D Piedrabuena, E
Jiménez-Ortega, N Miguez, FJ Plou, J Sanz-Aparicio, M
Fernández-Lobato Scientific
Reports (2021) 11, article number 7158 (doi: 10.1038/s41598-021-86568-6)
Structure and evolutionary trace-assisted screening of a residue
swapping the substrate ambiguity and chiral specificity in an esterase
I Cea-Rama, C Coscolín, P Katsonis, R Bargiela, N Golyshin,
O Lichtarge, M Ferrer, J Sanz-Aparicio Computational
and Structural Biotechnology Journal (2021) 19, 2307-2317(doi: 10.1016/j.csbj.2021.04.041)
Biochemical and Structural Characterization of a Novel Thermophilic
esterase EstD11 provides catalytic insights for HSL family Miguel-Ruano
V., Rivera I., Rajkovic J., Knapik K., Torrado A., Otero J.M.,
Beneventi E., Becerra M., Sánchez-Costa M., Hidalgo A.,
Berenguer J., Gonzalez-Siso M.I., Cruces J., Rúa
M.L., and
Hermoso J.A. Computational
and Structural
Biotechnology Journal (2021) 19, 1214-1232 (doi: 10.1016/j.csbj.2021.01.047)
Binding
of non-canonical peptidoglycan controls Vibrio cholerae broad spectrum
racemase activity Espaillat,
A., Carrasco-López, C., Bernardo-García, N.,
Rojas-Altuve, A., Klett, J., Morreale, A., Hermoso J.A., and
Cava F. Computational and Structural
Biotechnology Journal (2021) 19, 1119-1126(doi: 10.1016/j.csbj.2021.01.031)
Unconventional
Antibacterials and Adjuvants Chang, M., Mahasenan, K.
V.; Hermoso, J. A.; Mobashery, S. Accounts
of Chemical Research (2021) 54, 917–929(doi: 10.1021/acs.accounts.0c00776)
Identification
of ABA Receptor Agonists Using a Multiplexed High-Throughput Chemical
Screening
Jorge Lozano-Juste, Irene García-Maquilón,
José
Brea, Rocío Piña, Armando Albert, Pedro L.
Rodriguez,
María Isabel Loza
in Plant
Chemical Genomics. Methods in Molecular Biology (2021) vol. 2213,
Hicks G.R., Zhang C. (eds). Humana, New York, NY(doi:
10.1007/978-1-0716-0954-5_9)
Crystal structure and
pathophysiological role of the pneumococcal nucleoside-binding protein
PnrA
Mohammed
R. Abdullah, María T. Batuecas, Franziska Jennert,
Franziska Voß,
Philipp Westhoff, Thomas P. Kohler, Rafael Molina,
Stephanie
Hirschmann, Michael Lalk, Juan A. Hermoso, Sven Hammerschmidt Journal of
Molecular Biology (2021) 433, 166723(doi:10.1016/j.jmb.2020.11.022)
Structural
cues for understanding eEF1A2 moonlighting Alejandra A. Carriles;
Alberto Mills;
Maria-Jose Muñoz-Alonso; Juan M. Dominguez; Dolores
Gutiérrez; Juan A. Hermoso and Federico Gago Chembiochem
(2021) 22, 374
–391(doi:
10.1002/cbic.202000516)
2020
Environment-Sensitive Probes for
Illuminating Amyloid Aggregation In Vitro and in Zebrafish
Francisco
Fueyo-González, Juan A. González-Vera, Ibon
Alkorta, Lourdes Infantes,
M. Luisa Jimeno, Paula Aranda, Dario Acuña-Castroviejo, Alvaro
Ruiz-Arias, Angel Orte, Rosario Herranz ACS Sensors (2020) 5,
2792−2799 (doi:
10.1021/acssensors.0c00587)
Optimization
and comparison of statistical tools for the prediction of
multicomponent forms of a molecule: the antiretroviral Nevirapine as a
case study
Rogeria Nunes Costa, Duane Choquesillo-Lazarte, Silvia L. Cuffini, Elna
Pidcock, L. Infantes CrystEngComm (2020) 22, 7460-7474
(doi:
10.1039/D0CE00948B)
Smart lanthanide antennas for sensing water
Francisco
Fueyo-González, Emilio Garcia-Fernandez, David
Martínez, Lourdes
Infantes, Angel Orte, Juan A. González-Vera and
Rosario Herranz Chemical Communications (2020)
56, 5484-5487 (doi:
10.1039/d0cc01725f)
Fluorescence mechanism switching from ICT to PET by substituent
chemical manipulation: Macrophage cytoplasm imaging probes
Francisco
Fueyo-González, Juan A. González-Vera, Ibon
Alkorta, Lourdes Infantes,
M. Luisa Jimeno, Mar Fernández-Gutiérrez, M.
Carmen González-García,
Angel Orte, Rosario Herranz Dyes and Pigments (2020) 175,
108172 (doi: 10.1016/j.dyepig.2019.108172)
New multicomponent forms of the antiretroviral Nevirapine with improved
dissolution performance
Rogeria
Nunes Costa, Ana Lucía Reviglio, Sana Siedler, Simone
Gonçalves
Cardoso, Yamila Garro Linck, Gustavo Alberto Monti, Alexandre Magnus G.
Carvalho, Jackson Antônio Lamounier Camargos Resende, Marcelo
Henrique
da Cunha Chaves, Helvécio Vinícius Antunes Rocha,
Duane
Choquesillo-Lazarte, L.Infantes and Silvia L. Cuffini Crystal Growth & Design
(2020) 20-(2), 688-698 (doi: 10.1021/acs.cgd.9b01129)
Genetically
engineered proteins with two active sites for enhanced biocatalysis and
synergistic chemo- and biocatalysis.
S Alonso, G Santiago, I Cea-Rama, L
Fernández-López,
Coscolín, J Modregger, AK Ressmann, M
Martínez-Martínez, H Marrero, R Bargiela, M Pita,
JL
Gonzalez-Alfonso, ML Briand, D Rojo, C Barbas, FJ Plou, P N. Golyshin,
P Shahgaldian, J Sanz-Aparicio, V Guallar, and M Ferrer Nature
Catalysis (2020)3,
319–328(doi:10.1038/s41929-019-0394-4)
Tuning
the Properties of Natural
Promiscuous Enzymes by Engineering Their Nanoenvironment
Carolina I. Giunta, Isabel Cea-Rama, Sandra Alonso, Manon L. Briand,
Rafael Bargiela, Cristina Coscolín, Philippe F.-X. Corvini,
Manuel Ferrer, Julia Sanz-Aparicio, and Patrick Shahgaldian ACS Nano
(2020) 14, 17652–17664 (doi: 10.1021/acsnano.0c08716)
Structural analysis of the reducing-end xylose-releasing
exo-oligoxylanase Rex8A from Paenibacillus
barcinonensis BP-23 deciphers its molecular specificity
E Jiménez-Ortega, S Valenzuela, M
Ramírez-Escudero, FJ Pastor and J Sanz-Aparicio The Febs Journal (2020)287, 5362–5374
(doi: 10.1111/febs.15332)
Structural
characterization of the essential
cell division protein FtsE and its interaction with FtsX in Streptococcus pneumoniae Alcorlo, M.; Straume, D.;
Lutkenhaus, J.; Håvarstein, L.S. and Hermoso, J.A. mBio (2020) 11, e01488-20
(doi:10.1128/mBio.01488-20)
SEQUENCE
SLIDER: expanding poly-alanine fragments for phasing with multiple side
chain hypotheses R. Junqueira Borges, K.
Meindl, M.
Sammito, A. Medina, C. Millán, M. Alcorlo, J. A. Hermoso, M.
R.
de Mattos Fontes, I. Usón Acta
Crystallographica D (2020) D76, 221-237 (doi:10.1107/S2059798320000339)
Class A PBPs have a distinct and unique role in the construction of the
pneumococcal cell wall
Daniel Straume, Katarzyna Wiaroslawa Piechowiak, Silje Olsen, Gro Anita
Stamsås, Kari Helene Berg, Morten Kjos, Maria Victoria
Heggenhougen, Martín Alcorlo, Juan A. Hermoso, and Leiv
Sigve
Håvarstein Proceedings
of the National Academy of Sciences, PNAS (2020) 117,
6129-6138(doi:/10.1073/pnas.1917820117)
Recognition
and activation of the plant AKT1 potassium channel by the kinase CIPK23
Maria
Jose Sanchez-Barrena, Antonio Chaves-Sanjuan, Natalia Raddatz, Imelda
Mendoza, Alvaro Cortes, Federico Gago, Juana Maria Gonzalez-Rubio, Juan
Luis Benavente, Francisco J. Quintero, Jose M. Pardo, Armando Albert Plant
Physiology (2020) 182, 2143–2153 (doi:10.1104/pp.19.01084)
Catalytic Cycle of Glycoside Hydrolase BglX from Pseudomonas aeruginosa
and Its Implication in Biofilm Formation Mahasenan,
K.; Batuecas, M.; De Benedetti, S.; Kim, C.; Rana, N.; Lee, M.; Hesek,
D.; Fisher, J.; Sanz-Aparicio, J.; Hermoso, J. A.; Mobashery, S. ACS
Chemical Biology (2020) 15, 189-196 (doi:10.1021/acschembio.9b00754)
The cryo-EM structure of Thermotoga maritima
β-galactosidase: quaternary structure guides protein
engineering.
Míguez-Amil S, Jiménez-Ortega E,
Ramírez-Escudero
M, Talens-Perales D, Marín-Navarro J, Polaina J,
Sanz-Aparicio
J, Fernández-Leiro R ACS
Chemical Biology (2020) 15, 179-188 (doi:10.1021/acschembio.9b00752)
2019
A structural study of new
tetrakis(1H-pyrazol-1-yl)methanes
V.L.M.Silva, A.M.S.Silva, R.M.Claramunt, C.López, D.Sanz,
L.Infantes, A.Martínez-López, F.Reviriego,
I.Alkorta and
J.Elguero Tetrahedron (2019) 75,
130690-130700 (doi:
10.1016/j.tet.2019.130690)
Chiral
Microneedles from an Achiral Bis(boron dipyrromethene): Spontaneous
Mirror Symmetry Breaking Leading to a Promising Photoluminescent
Organic Material
L.Gartzia-Rivero, C.Ray, E.M.Sanchez-Carnerero,
J.Bañuelos, F.Moreno-Jiménez, B.Lora-Maroto,
I.Garcia-Moreno,
L.Infantes, B.Mendez, I.Lopez-Arbeloa, S.De la Moya Langmuir
(2019) 35, 5021-5028 (doi: 10.1021/acs.langmuir.9b00409)
Structural
basis of denuded glycan recognition by SPOR domains in bacterial cell
division
Martín Alcorlo, David A. Dik, Stefania De Benedetti, Kiran
V.
Mahasenan, Mijoon Lee, Teresa Domínguez-Gil, Dusan Hesek,
Elena
Lastochkin, Daniel López, Bill Boggess, Shahriar Mobashery
&
Juan A. Hermoso Nature Communications (2019) 10,
Article number: 5567(doi:10.1038/s41467-019-13354-4)
Deciphering
the molecular specificity of phenolic compounds as inhibitors or
glycosyl acceptors of β-fructofuranosidase from Xanthophyllomyces dendrorhous
Mercedes
Ramirez-Escudero, Noa Miguez, Maria Gimeno-Perez, Antonio O.
Ballesteros, Maria Fernandez-Lobato, Francisco J. Plou and
Julia
Sanz-Aparicio Scientific
Reports (2019) 9:17441 (doi:10.1038/s41598-019-53948-y)
Disulfide
Engineered Lipase to Enhance the Catalytic Activity: A Structure-Based
Approach on BTL2 C.A. Godoy, J. Klett, B. Di
Geronimo, J. A. Hermoso, J. M.
Guisán, C. Carrasco-López International
Journal of Molecular Sciences (2019) 20, 5245 (doi:10.3390/ijms20215245)
PYR/PYL/RCAR
ABA receptors
Pedro L. Rodriguez, Jorge Lozano-Juste, Armando Albert Advances
in Botanical Research (2019) 92, 51-82 (doi:10.1016/bs.abr.2019.05.003)
Structural basis of the inhibition of GH1 β-glucosidases by
multivalent pyrrolidine iminosugars
M Martínez-Bailén, E Jiménez-Ortega,
AT Carmona, I
Robina, J Sanz-Aparicio, D Talens-Perales, J Polaina, C Matassini, F
Cardona and A J Moreno-Vargas Bioorganic
Chemistry (2019) 89, 103026-103036(doi:10.1016/j.bioorg.2019.103026)
Insights into real-time chemical processes in a calcium sensor
protein-directed dynamic library
Andrea Canal-Martín, Javier Sastre, María
José
Sánchez-Barrena, Angeles Canales, Sara Baldominos, Naiara
Pascual, Loreto Martínez-González, Dolores
Molero,
Mª Encarnación Fernández-Valle, Elena
Sáez,
Patricia Blanco-Gabella, Elena Gómez-Rubio, Sonsoles
Martín-Santamaría, Almudena Sáiz,
Alicia Mansilla,
F. Javier Cañada, Jesús
Jiménez-Barbero, Ana
Martínez & Ruth Pérez-Fernández Nature
Communications (2019) 10, Article number: 2798 (doi:10.1038/s41467-019-10627-w)
The structure
of full-length human phenylalanine hydroxylase in complex with
tetrahydrobiopterin Marte Innselset Flydal,
Martin Alcorlo, Fredrik Gullaksen Johannessen, Siseth
Martinez-Caballero, Lars Skjærven, Rafael Fernandez-Leiro,
Aurora Martinez, Juan A. Hermoso Proceedings
of the National Academy of Sciences, PNAS (2019) 116,
11229-11234(doi:10.1073/pnas.1902639116)
Pyrrolopyrimidine
vs Imidazole-Phenyl-Thiazole Scaffolds in Nonpeptidic Dimerization
Inhibitors of Leishmania
infantumTrypanothione Reductase
A. Revuelto, M. Ruiz-Santaquiteria, H. de Lucio, A. Gamo, A. A.
Carriles,
K. Jesús Gutiérrez, P. A.
Sánchez-Murcia, J. A. Hermoso, F. Gago, M.-J.
Camarasa, A. Jiménez-Ruiz, and S. Velázquez ACS
Infectious Diseases (2019) 5, 873-891 (doi:10.1021/acsinfecdis.8b00355)
Macromolecular
Machineries in Cell-wall Recycling Batuecas, María
T and Hermoso, Juan A In eLS
(2019). John
Wiley & Sons, Ltd: Chichester (doi:10.1002/9780470015902.a0028397)
Cold-induced aldimine bond cleavage by Tris in Bacillus subtilis
alanine racemase
Noelia Bernardo-García, Pedro Alejandro
Sánchez-Murcia, Akbar Espaillat, Carol
Siseth Martínez-Caballero, Felipe Cava, Juan Hermoso and
Federico
Gago
Organic
& Biomolecular Chemistry (2019) 17, 4350-4358 (doi:10.1039/C9OB00223E)
The
quinazolinone allosteric inhibitor of
PBP2a synergizes with piperacillin and tazobactam against
methicillin-resistant Staphylococcus
aureus Janardhanan, J.; Bouley,
R.;
Martínez-Caballero, S.; Peng, Z.; Batuecas-Mordillo, M.;
Meisel,
J. E.; Ding, D.; Schroeder, V. A.; Wolter, W. R.; Mahasenan, K. V.;
Hermoso, J. A.; Mobashery, S.; Chang, M. Antimicrob.
Agents Chemother. (2019) 63, e02637-18 (doi:10.1128/AAC.02637-18)
Structure of the large extracellular loop of
FtsX and its interaction with the essential peptidoglycan hydrolase
PcsB in Streptococcus
pneumoniae Britta E. Rued,
Martín Alcorlo,
Katherine A. Edmonds, Siseth Martínez-Caballero, Daniel
Straume,
Yue Fu, Kevin E. Bruce, Hongwei Wu, Leiv Sigve Håvarstein,
Juan A. Hermoso, Malcolm E. Winkler and David P. Giedroc mBio
(2019) 10, e02622-18 (doi:10.1128/mBio.02622-18)
Rayos X: hallazgo y consecuencias de una luz
prodigiosa
Martín Martínez-Ripoll CIENCIA, y un gran paso para la
humanidad!!! (2019) (link
to the chapter)
2018
Characterization of an atypical, thermostable, organic solvent- and
acid-tolerant 2'-deoxyribosyltransferase from Chroococcidiopsis thermalis Del Arco J,
Sánchez-Murcia PA, Mancheño JM, Gago F,
Fernández-Lucas J Applied Microbiology and
Biotechnology (2018) 102, 6947-6957 (doi:
10.1007/s00253-018-9134-y)
Identification
of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum
subsp. Polymorphum Tomás-Cortázar
J, Plaza-Vinuesa
L, de Las Rivas B, Lavín JL, Barriales D, Abecia L,
Mancheño JM, Aransay AM, Muñoz R, Anguita J,
Rodríguez H Microbial Cell
Factory (2018) 17, 33 (doi:
10.1186/s12934-018-0880-4)
2'-Deoxyribosyltransferase
from Bacillus
psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for
the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium Fresco-Taboada A,
Fernández-Lucas J, Acebal C, Arroyo M, Ramón F,
de la Mata I, Mancheño JM Catalysts (2018) 8, 8 (doi:
10.3390/catal8010008)
Enzymatic Synthesis of Therapeutic
Nucleosides using a Highly Versatile Purine Nucleoside 2
'-DeoxyribosylTransferase from Trypanosoma
brucei
Pérez E, Sánchez-Murcia P, Jordaan J, Blanco MD,
Mancheño JM, Gago F, Fernández-Lucas J. Chemcatchem
(2018) 10, 4406-416(doi:10.1002/cctc.201800775)
Characterization of an atypical, thermostable, organic solvent- and
acid-tolerant 2'-deoxyribosyltransferase from Chroococcidiopsis thermalis
Del Arco J, Sánchez-Murcia PA, Mancheño JM, Gago
F, Fernández-Lucas J. Applied
Microbiology and Biotechnology (2018) 102, 6947-6957 (doi:10.1007/s00253-018-9134-y)
Identification of a highly active tannase enzyme from the oral pathogen
Fusobacterium
nucleatum subsp. Polymorphum
Tomás-Cortázar
J, Plaza-Vinuesa L, de Las Rivas B, Lavín JL, Barriales D,
Abecia L,
Mancheño JM, Aransay AM, Muñoz R, Anguita J,
Rodríguez H. Microbial
Cell Factories (2018) 17:33(doi:10.1186/s12934-018-0880-4)
2'-Deoxyribosyltransferase from Bacillus
psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for
the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
Fresco-Taboada A, Fernández-Lucas J, Acebal C, Arroyo M,
Ramón F, de la Mata I, Mancheño JM. Catalysts
(2018) 8, 8
(doi:10.3390/catal8010008)
Yeast
cultures expressing the Ffase from Schwanniomyces
occidentalis,
a simple system to produce the potential prebiotic sugar 6-kestose
D. Rodrigo-Frutos, D. Piedrabuena, J. Sanz-Aparicio, M.
Fernández-Lobato Applied Microbiology and
Biotechnology (2018) 103, 279–289
(doi:10.1007/s00253-018-9446-y)
Structural Insights into the Substrate Promiscuity of a
Laboratory-Evolved Peroxygenase
Mercedes Ramirez-Escudero, Patricia Molina-Espeja, Patricia Gomez de
Santos, Martin Hofrichter, Julia Sanz-Aparicio and Miguel Alcalde ACS
Chemical Biology (2018) 13, 3259–3268(doi:10.1021/acschembio.8b00500)
A Structural Dissection of the Active Site of the Lytic
Transglycosylase MltE from Escherichia coli
David A. Dik, María T. Batuecas, Mijoon Lee, Kiran V.
Mahasenan,
Daniel R. Marous, Elena Lastochkin, Jed F. Fisher, Juan A. Hermoso, and
Shahriar Mobashery Biochemistry
(2018) 57, 6090−6098 (doi:10.1021/acs.biochem.8b00800)
Fructosylation
of hydroxytyrosol by the β-fructofuranosidase from
Xanthophyllomyces dendrorhous: Insights into the molecular basis of the
enzyme specificity
Miguez M, Ramírez-Escudero M, Gimeno-Pérez M,
Poveda A,
Jiménez-Barbero J, Ballesteros AO,
Fernández-Lobato
M, Sanz-Aparicio J, and Plou FJ ChemCatChem (2018) 10,
4878–4887 (doi:10.1002/cctc.201801171)
Deciphering the inhibition of the neuronal
calcium sensor 1 and the guanine exchange factor Ric8a with a small
phenothiazine molecule for the rational generation of therapeutic
synapse function regulators Roca
C, Martínez-González L, Daniel-Mozo M, Sastre J,
Infantes L, Mansilla
A, Chaves-Sanjuán A, González-Rubio JM, Gil C,
Cañada J, Martínez
A, Sánchez-Barrena MJ and Campillo NE Journal of Medicinal Chemistry
(2018) Article ASAP (doi:10.1021/acs.jmedchem.8b00088)
Use
of chitin and chitosan to produce new
chitooligosaccharides by chitinase Chit42: enzymatic activity and
structural basis of protein specificity
PE Kidibule, P Santos
Moriano, E Jiménez Ortega, M Ramírez Escudero, MC
Limón, M Remacha, FJ
Plou, J Sanz Aparicio and M Fernández Lobato Microbial Cell Factories (2018)
17:47 (doi:10.1186/s12934-018-0895-x)
Exploiting
distant homologs for phasing
through generation of compact fragments, local fold refinement and
partial solution combination Millán,
C.; Sammito, M.D.; McCoy, A.J.; Nascimento, A.F.Z.; Petrillo, G.;
Oeffner, R.D.; Domínguez-Gil, T.; Hermoso, J.A.; Read, R.J;
and Usón,
I. Acta
Crystallographica D (2018) D74, 290-304(doi:10.1107/S2059798318001365)
Exolytic
and Endolytic Turnover of
Peptidoglycan by Lytic Transglycosylase Slt of Pseudomonas aeruginosa Lee, M.,
Batuecas, M.T., Tomoshige, S., Domínguez-Gil, T., Mahasenan,
K.V., Dik, D.A., Hesek, D., Millán, C., Usón, I.,
Lastochkin, E., Hermoso, J.A., Mobashery, S.
Proceedings
of the National Academy of Sciences, PNAS (2018)115, 4393-4398 (doi:10.1073/pnas.1801298115)
Structural Biology of Salt and Drought Tolerance in Plants
Sánchez-Barrena, María J; Martinez-Ripoll,
Martín; and Albert, Armando
In eLS
(2018). John
Wiley & Sons, Ltd: Chichester (doi:
10.1002/9780470015902.a0027628)
Mechanism of the Escherichia
coli MltE lytic transglycosylase, the
cell-wall-penetrating
enzyme for Type VI secretion system assembly
Byungjin Byun, Kiran V. Mahasenan, David A. Dik, Daniel R. Marous,
Enrico Speri, Malika Kumarasiri, Jed F. Fisher, Juan A. Hermoso and
Shahriar Mobashery Scientific
Reports (2018) 8, Article number 4110 (doi:10.1038/s41598-018-22527-y)
Noncatalytic aspartate at the exonuclease
domain of proofreading DNA polymerases regulates both degradative and
synthetic activities
Del Prado A, Franco-Echevarría E, González B,
Blanco L, Salas M, de Vega M. Proceedings
of the National Academy of Sciences, PNAS (2018)115, E2921-E2929
(doi:10.1073/pnas.1718787115)
Structural
insights into the
binding and catalytic mechanisms of the Listeria monocytogenes
bacteriophage glycosyl hydrolase PlyP40
Romero, P.; Bartual, S.G.; Schmelcher, M.; Glück, C.; Hermoso,
J.A. and Loessner, M.J. Molecular
Microbiology (2018)108,
128-142(doi:10.1111/mmi.13922)
Allostery,
Recognition of Nascent Peptidoglycan and Crosslinking of the Cell Wall
by the Essential Penicillin-Binding Protein 2x of Streptococcus
pneumoniae Bernardo-Garcia, N.;
Mahasenan, K.;
Batuecas, M.T.; Lee, M.; Hesek, D.; Petrácková,
D.;
Doubravová, L.; Branny, P.; Mobashery, S.; Hermoso, J.A.
ACS Chemical
Biology (2018) 13, 694–702 (doi:10.1021/acschembio.7b00817)
CIENCIA, y yo quiero ser...
Cristalógrafo!!!
Martín Martínez-Ripoll CIENCIA,
y yo quiero ser científico!!! (2018) (link
to the chapter)
Three-dimensional
structures of Lipoproteins from Streptococcus
pneumoniae and Staphylococcus
aureus
Bartual, S.G; Alcorlo, M.; Martínez-Caballero, S.; Molina,
R. and Hermoso, J.A. International
Journal of Medical Microbiology (2018) 308, 692-704(doi:10.1016/j.ijmm.2017.10.003) 2017 The
structure
of transcription termination factor Nrd1 reveals an original mode for
GUAA recognition
Franco-Echevarría
E, González-Polo N, Zorrilla S,
Martínez-Lumbreras S, Santiveri CM,
Campos-Olivas R, Sánchez M, Calvo O, González B
& Pérez-Cañadillas
JM Nucleic Acids
Research (2017) 45, 10293-10305 (doi:10.1093/nar/gkx685)
The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate
2-kinase reveals a new zinc-binding site and key features for protein
function
Franco-Echevarría E, Sanz-Aparicio J, Brearley CA,
González-Rubio JM, González B.
Journal of Biological Chemistry (2017) 292, 10534-10548(doi:10.1074/jbc.M117.780395)
Crystallization and Preliminary X-Ray Diffraction Analysis of a Mammal
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
Franco-Echevarría E, Sanz-Aparicio J, Troffer-Charlier N,
Poterszman A, González B Protein
Journal (2017) 36, 240(doi:10.1007/s10930-017-9717-y)
Deciphering
how Cpl-7 cell wall-binding repeats recognize the bacterial
peptidoglycan
N. Bustamante, M. Iglesias-Bexiga, N. Bernardo-García, N.
Silva-Martín, G. García, M. A. Campanero-Rhodes,
E. García, I. Usón, R. M. Buey, P.
García, J. A.
Hermoso, M. Bruix, M. Menéndez Scientific
Reports (2017) 7, Article number 16494(doi:10.1038/s41598-017-16392-4)
X-ray
Structure of Catenated Lytic Transglycosylase SltB1
Teresa Domínguez-Gil, Rafael Molina, David A. Dik, Edward
Spink, Shahriar Mobashery, and Juan A. Hermoso Biochemistry
(2017) 56, 6317-6320 (doi:10.1021/acs.biochem.7b00932)
The structure of ligand-bound intermediates of crop ABA receptors
highlights the role of the PP2C as necessary ABA co-receptor
Moreno-Alvero M, Yunta C, Gonzalez-Guzman M, Lozano-Juste J,
Benavente JL, Arbona V, Menéndez M, Martinez-Ripoll M,
Infantes
L, Gomez-Cadenas A, Rodriguez PL, Albert A Molecular
Plant (2017) 10, 1250–1253(doi:10.1016/j.molp.2017.07.004)
Structural
basis of the substrate specificity and instability in solution of a
glycosidase from Lactobacillus
plantarum
Acebrón I, Plaza-Vinuesa L, de Las Rivas B, Muñoz
R, Cumella J, Sánchez-Sancho F, Mancheño JM Biochimica et Biophysica
Acta (2017) 1865, 1227-1236 (doi:10.1016/j.bbapap.2017.07.007)
2'-Deoxyribosyltransferase
from Leishmania mexicana,
an efficient biocatalyst for one-pot,
one-step synthesis of nucleosides from poorly soluble purine bases
Crespo, N., Sánchez-Murcia, P.A., Gago, F., Cejudo-Sanches,
J., Galmes, M.A., Fernández-Lucas, J., Mancheño,
J.M. Applied Microbiology and
Biotechnology (2017) 101, 7187–7200 (doi:10.1007/s00253-017-8450-y)
A
single amino
acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis
serotypes D. Roy, T. Athey, J.-P.
Auger, G.
Goyette-Desjardins, M.-R. Van Calsteren, D. Takamatsu, M. Okura, S.
Teatero, M. Alcorlo, J.A. Hermoso, M. Segura, M. Gottschalk, and N.
Fittipaldi Scientific
Reports (2017) 7, Article number 4066 (doi:10.1038/s41598-017-04403-3)
40
Años de Cristalografía en el Rocasolano
Martín Martínez Ripoll 100cias@uned,
Facultad de Ciencias, nº 10 (2017) (link
to the article) [PDF-copy]
Catalytic Cycle of the
N-Acetylglucosaminidase NagZ from Pseudomonas
aeruginosa
Acebron, I.; Mahasenan, K.; De Benedetti, S.; Lee, M.; Artola-Recolons,
C.; Hesek, D.; Wang, H.; Hermoso, J. A.; Mobashery, S. Journal of the American
Chemical Society (2017) 139, 6795-6798 (doi:10.1021/jacs.7b01626)
Structural features of Aspergillus
niger β-galactosidase define its activity against
glycoside linkages
Rico-Díaz, A., Ramírez-Escudero, M.,
Vizoso-Vázquez, A., Cerdán, M.E., Becerra, M. and
Sanz-Aparicio, J. The
FEBS Journal (2017) 284, 1815–1829(doi:10.1111/febs.14083)
Conformational
dynamics in penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus,
allosteric communication network and enablement
of catalysis Mahasenan,
K.; Molina, R.; Bouley, R.;
Batuecas, M.; Fisher, J.; Hermoso, J.A.; Chang, M. and Mobashery, S. Journal of the
American Chemical Society (2017) 139,
2102-2110 (doi:10.1021/jacs.6b12565)
Interference
of the complex between NCS-1 and Ric8a with phenothiazines regulates
synaptic function and is an approach for fragile X syndrome
Alicia Mansilla, Antonio Chaves-Sanjuan, Nuria E. Campillo, Ourania
Semelidou, Loreto Martínez-González, Lourdes
Infantes, Juana María González-Rubio, Carmen Gil,
Santiago
Conde, Efthimio M. C. Skoulaki, Alberto Ferrús, Ana
Martínez, María José
Sánchez-Barrena Proceedings
of the National Academy of Sciences, PNAS (2017) 114, E999–E1008
(doi:10.1073/pnas.1611089114)
Muropeptide
Binding and the X-Ray Structure of the
Effector Domain of the Transcriptional Regulator AmpR of Pseudomonas aeruginosa Dik, D. A., Domínguez-Gil, T., Lee, M.,
Hesek, D.,
Byun, B., Fishovitz, J., Boggess, B., Hellman, L. M., Fisher, J. F.,
Hermoso, J. A., Mobashery, S. Journal of the
American Chemical Society (2017)139, 1448–1451
(doi:10.1021/jacs.6b12819)
Carbohydrate recognition and lysis by bacterial
peptidoglycan hydrolases
Martin Alcorlo, Siseth Martinez-Caballero, Rafael Molina and Juan A
Hermoso Current
Opinion in Structural Biology (2017) 44, 87–100(doi:10.1016/j.sbi.2017.01.001)
2016
Modular Architecture and Unique Teichoic Acid Recognition Features of
Choline-Binding Protein L (CbpL) Contributing to Pneumococcal
Pathogenesis
J. Gutiérrez-Fernández, M. Saleh, M. Alcorlo, A.
Gómez
M., D. Pantoja-Uceda, M. A. Treviño, F. Voß, M. R.
Abdullah, S. Galán-Bartual, J. Seinen, P. A.
Sánchez-Murcia, F. Gago, M. Bruix, S.
Hammerschmidt and J. A. Hermoso Scientific
Reports (2016) 6, Article Number 38094(doi:10.1038/srep38094)
Oriented Attachment of
Recombinant Proteins to Agarose-Coated Magnetic Nanoparticles by Means
of a β-Trefoil Lectin Domain
Iván Acebrón,Amalia G. Ruiz-Estrada, Yurena
Luengo,
María del Puerto Morales,José Manuel
Guisán, and
José Miguel Mancheño Bioconjugate
Chemistry (2016) 27, 2734−2743 (doi:10.1021/acs.bioconjchem.6b00504)
Átomos y moléculas de cristal
Martín Martinez-Ripoll conCIENCIAS.digital
(2016) 18, 24-35(link
to the issue)[PDF-copy]
Structural bioinformatics in broad-spectrum racemases: a new path in
antimicrobial research
Noelia Bernardo-García, Pedro Sánchez-Murcia,
Federico Gago, Felipe Cava and Juan A. Hermoso Current
Organic Chemistry (2016) 20, 1222-1231(doi:10.2174/1385272819666150810213115)
Activation
by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic
Transglycosylase from Pseudomonas
aeruginosa T.
Domínguez-Gil, M. Lee, I. Acebrón-Avalos, K. V.
Mahasenan, D. Hesek, D. A. Dik, B. Byun, E. Lastochkin, J. F. Fisher,
S. Mobashery and J. A. Hermoso Structure
(2016) 24, 1729-1741 (doi:10.1016/j.str.2016.07.019)
Structural
and functional characterization of a ruminal β-glycosidase
defines a novel subfamily of glycosyl hydrolase family 3 with permuted
domain topology
Mercedes Ramírez-Escudero, Mercedes V. del Pozo, Julia
Marín-Navarro, Beatriz González, Peter N.
Golyshin, Julio Polaina, Manuel Ferrer and Julia Sanz-Aparicio Journal of
Biological Chemistry(2016) 291,
24200-24214
(doi:10.1074/jbc.M116.747527)
The glycoside hydrolase family 8 reducing-end xylose-releasing
exo-oligoxylanase Rex8a from Paenibacillus
barcinonensis BP-23 is active on branched
xylooligosaccharides Susana Valenzuela, Sergi
López, S., Peter Biely, Julia Sanz-Aparicio, F. Javier Pastor Applied
and Environmental Microbiology (2016) 82, 5116-5124 (doi:10.1128/AEM.01329-16)
Molecular
characterization and heterologous expression of a Xanthophyllomyces
dendrorhous α-glucosidase with potential for
prebiotics production Patricia
Gutiérrez-Alonso, María Gimeno-Pérez,
Mercedes Ramírez-Escudero,
Francisco J. Plou, Julia Sanz-Aparicio, María
Fernández-Lobato Applied
Microbiology and Biotechnology (2016) 100, 3125-3135(doi: 10.1007/s00253-015-7171-3)
Renew
or Die: The molecular mechanisms of peptidoglycan recycling and
antibiotic resistance in Gram-negative pathogens
T. Domínguez-Gil, R. Molina, M. Alcorlo and J. A. Hermoso Drug
Resistance Updates (2016) 28, 91-104(doi:10.1016/j.drup.2016.07.002) Orthologous
and paralogous AmpD
Peptidoglycan Amidases from
Gram-Negative Bacteria
Ivanna Rivera, Rafael Molina, Mijoon Lee, Shariar Mobashery and Juan A.
Hermoso Microbial
Drug Resistance (2016) 22,470-476,
Great Wall 2015 Article (doi:10.1089/mdr.2016.0083)
Turnover
of Bacterial Cell Wall by SltB3, a Multidomain Lytic Transglycosylase
of Pseudomonas aeruginosa Lee, M.;
Domínguez-Gil, T.; Hesek, D.; Mahasenan, K. V.;
Lastochkin, E.; Hermoso, J. A.; Mobashery, S. ACS
Chemical Biology (2016) 11, 1525-1531 (doi:10.1021/acschembio.6b00194)
The
structures of two scorpionates: Thallium
tetrakis(3-phenyl-1H-pyrazol-1-yl)borate and potassium
tetrakis(3-cyclopropyl-1H-pyrazol-1-yl)borate Infantes, L.; Claramunt,
R.M.; Sanz, D.; Alkorta, I.; Elguero, J. Acta
Crystallographica (2016) C72, 819-825 (doi:10.1107/S2053229616007385) Structural
insights into the synthesis of FMN in prokaryotic organisms
Beatriz Herguedas, Isaías Lans, Juan
A.
Hermoso, Marta Martínez-Júlvez
and M. Medina Acta
Crystallographica (2016) D71, 2526-2542(doi:10.1107/S1399004715019641)
Two-Photon Fluorescence Anisotropy Imaging to Elucidate the Dynamics
and the Stability of Immobilized Proteins
Orrego AH, García C, Manchen~o JM,
Guisa´n JM,
Lillo MP, López-Gallego F Journal
of Physical Chemistry B (2016) 120, 485-491 (doi:10.1021/acs.jpcb.5b12385)
Structural
analysis of β-fructofuranosidase
from Xanthophyllomyces
dendrorhous
reveals unique features and the crucial role of N-glycosylation in
oligomerization and activity
M Ramírez-Escudero, M Gimeno-Pérez, B
González, D
Linde, Z Merzdo, M Fernández-Lobato, J Sanz-Aparicio Journal of
Biological Chemistry(2016) 291,
6843-6857(doi:10.1074/jbc.M115.708495) Calcium-dependent
oligomerization of CAR proteins at cell membrane modulates ABA signaling
Maira Diaza, Maria Jose Sanchez-Barrena, Juana Maria Gonzalez-Rubio,
Lesia Rodriguez, Daniel Fernandez, Regina Antoni, Cristina Yunta, Borja
Belda-Palazon, Miguel Gonzalez-Guzman, Marta Peirats-Llobet, Margarita
Menendez, Jasminka Boskovic, Jose A. Marquez, Pedro L. Rodriguez, and
Armando Albert Proceedings
of the National Academy of Sciences, PNAS (2016) 113,
E396-E405 (doi:
10.1073/pnas.1512779113)
2015 Synthesis of Enantiopure
3-Hydroxypiperidines
from Sulfinyl Dienyl Amines by Diastereoselective Intramolecular
Cyclization and [2,3]-Sigmatropic Rearrangement
Simal, C.; Bates, R.H.; Urena, M.; Giménez, I.; Koutsou, C.;
Fernández De La Pradilla, R.; Viso, A.; Infantes, L. Journal of Organic Chemistry
(2015) 80, 7674-7692 (doi:10.1021/acs.joc.5b01307)
Prediction of
H-bonding motifs for pyrazoles and oximes using the Cambridge
structural database
Infantes, L.; Motherwell, S.
in Science
of Crystal Structures: Highlights in Crystallography (2015) 269-280,
Springer
International Publishing (doi:10.1007/978-3-319-19827-9_28) Structural
insights into the
synthesis of FMN in prokaryotic organisms
Beatriz Herguedas, Isaías Lans, Juan
A.
Hermoso, Marta Martínez-Júlvez
and M. Medina Acta
Crystallographica (2015) D71, 2526-2542(doi:10.1107/S1399004715019641)
Molecular
characterization and heterologous expression of a Xanthophyllomyces
dendrorhous α-glucosidase with potential for
prebiotics production Patricia
Gutiérrez-Alonso, María Gimeno-Pérez,
Mercedes
Ramírez-Escudero, Francisco J. Plou, Julia Sanz-Aparicio,
María Fernández-Lobato Applied
Microbiology and Biotechnology (2015) 100, 3125-3135(doi:
10.1007/s00253-015-7171-3)
Celebrating
100 years of modern crystallography
Mancheño, J.M. (coord) Arbor
(2015) 191 (772): a223 (doi:
10.3989/arbor.2015.i772)
Presentation
Mancheño, J.M. in Celebrating
100 years of modern crystallography. (Mancheño,
J.M., coord.), Arbor (2015) 191 (772): a223 [PDF-copy]
Personal perspectives Mancheño,
J.M. in Celebrating
100 years of modern crystallography. (Mancheño,
J.M., coord.), Arbor (2015) 191 (772): a223 [PDF-copy]
The legacy of women to
crystallography Sanz-Aparicio,
J. in Celebrating
100 years of modern crystallography. (Mancheño,
J.M., coord.), Arbor (2015) 191 (772): a223 [PDF-copy] The new InsP3Kinase inhibitor
BIP4 is competitive to InsP3 and blocks proliferation and adhesion of
lung cancer cells
Schröder D, Tödter K, Franco-Echevarría,
E, Gonzalez B, Rohaly G and Sabine Windhorst Biochemical
Pharmacology (2015) 96, 143-150 (doi:
10.1016/j.bcp.2015.05.004)
Enantioselective oxidation of galactitol 1-phosphate by
galactitol-1-phosphate 5-dehydrogenase from Escherichia coli
Rocío Benavente, María Esteban-Torres,
Gert-Wieland
Kohring, Álvaro Cortés-Cabrera, Pedro A.
Sánchez-Murcia, Federico Gago, Iván
Acebrón,
Blanca de las Rivas, Rosario Muñoz and José M.
Mancheño Acta
Crystallographica (2015) D71, 1540-1554(doi:
10.1107/S1399004715009281)
Exploring
multimodularity in plant cell wall deconstruction: structural and
functional analysis of Xyn10C containing the CBM22-1-CBM22-2 tandem
M. Angela Sainz-Polo, Beatriz González, Margarita
Menéndez, F.I. Javier Pastor and Julia Sanz-Aparicio Journal
of Biological Chemistry (2015) 290, 17116-17130(doi:10.1074/jbc.M115.659300)
Structure and
functions of choline binding proteins Galán-Bartual
S., Pérez-Dorado I., García P. and Hermoso J.A. In Streptococcus
Pneumoniae Molecular Mechanisms of Host-Pathogen Interactions (2015),
Chap. 11, pp. 207-230. Jeremy Brown, Sven Hammerschmidt and Carlos
Orihuela Eds., Elsevier. ISBN:
978-0-12-410530-0
Synthesis of a heterogeneous artificial metallolipase with chimeric
catalytic activity
M. Filice, O. Romero, J. Gutiérrez-Fernández, B.
de las Rivas, J. A. Hermoso and J. M. Palomo Chemical
Communications (2015) 51, 9324-9327(doi:10.1039/C5CC02450A)
The Allosteric
Site for the Nascent Cell Wall in Penicillin-Binding
Protein 2a: an Achilles’ Heel of Methicillin-Resistant
Staphylococcus aureus Ivan Acebrón,
Mayland Chang, Shahriar Mobashery and Juan A. Hermoso Current
Medicinal Chemistry (2015) 22, 1678-1686(doi:10.2174/0929867322666150311150215)
The bacteriocin AS-48 requires dimer dissociation followed by
hydrophobic interactions with the membrane for antibacterial activity.
Cebrián R, Martínez-Bueno M, Valdivia E, Albert
A, Maqueda M, Sánchez-Barrena MJ. Journal
of Structural Biology (2015) 190, 162–172(doi:10.1016/j.jsb.2015.03.006)
Molecular recognition of PTS-1 cargo proteins by Pex5p: implications
for protein mistargeting in primary hyperoxaluria
Mesa-Torres N, Tomic N, Albert A, Salido E, Pey AL. Biomolecules
(2015) 5, 121-141(doi:10.3390/biom5010121)
Discovery of Antibiotic (E)-3-(3-Carboxyphenyl)-2-(4-cyanostyryl)quinazolin-4(3H)-one
Renee Bouley, Malika Kumarasiri, Zhihong Peng, Lisandro H.Otero, Wei
Song, Mark A.Suckow, Valerie A.Schroeder, William R.Wolter, Elena
Lastochkin, Nuno T.Antunes, Hualiang Pi, Sergei Vakulenko, Juan
A.Hermoso, Mayland Chang, and Shahriar Mobashery Journal
of the American Chemical Society (2015) 137, 1738−1741(doi:10.1021/jacs.5b00056)
Experimental
and
Theoretical Studies on the Rearrangement of 2-Oxoazepane
α,α-Amino Acids into 2’-Oxopiperidine
β2,3,3-AminoAcids: An Example of
Intramolecular Catalysis
Núñez-Villanueva,
D.; Bonache, M. A.; Lozano, L.; Infantes, L.; Elguero, J.; Alkorta, I.;
García-López, M.T.;
González-Muñiz, R.;
Martín-Martínez, M. Chemistry
- A European Journal (2015) 21, 2489-2500(doi:10.1002/chem.201405640)
The Reaction of 2-Amino-4H-pyrans with
N-Bromosuccinimide.
Samadi, A.; Silva, D.; Chioua, M.; Infantes, L.; Soriano, E.;
Marco-Contelles, J. Molecular
Diversity (2015) 19, 103-122(doi:10.1007/s11030-014-9560-4) 2014
Silver Triflate-Catalyzed Cyclization of
2-Amino-6-propargyl-amineazines Leading to Iminoimidazoazines
Chioua, M.; Samadi, A.; Soriano, E.; Infantes, L.; Marco-Contelles, J. Advanced
Synthesis and Catalysis (2014) 356, 1235-1241(doi:10.1002/adsc.201300799)
Characterization of a cold-active esterase from Lactobacillus plantarum
suitable for food fermentations
Esteban-Torres, M.; Mancheño, J.M.; de las Rivas, B.;
Muñoz, R. Journal
of Agricultural and Food Chemistry (2014) 62, 5126-5132(doi:10.1021/jf501493z)
Characterization of a versatile arylesterase from Lactobacillus plantarum
active on wine esters
Esteban-Torres, M.; Barcenilla, J.M.; Mancheño, J.M.; de las
Rivas, B.; Muñoz, R. Journal
of Agricultural and Food Chemistry (2014) 62, 5118-5125 (doi:10.1021/jf500991m)
Production and characterization of a tributyrin esterase from Lactobacillus plantarum
suitable for cheese lipolysis
Esteban-Torres, M.; Mancheño. J.M.; de las Rivas, B.;
Muñoz, R. Journal
of Dairy Science (2014) 97, 6737-6744(doi:10.3168/jds.2014-8234)
A new calmodulin binding motif for inositol 1,4,5-trisphosphate
3-kinase regulation
Franco-Echevarria, E., Baños-Sanz, J.I., Monterroso, B.,
Round A, Sanz-Aparicio, J., González, B. Biochemical
Journal (2014) 463, 319-328 (doi:10.1042/BJ20140757)
C2-Domain
Abscisic Acid-Related Proteins Mediate the Interaction of PYR/PYL/RCAR
Abscisic Acid Receptors with the Plasma Membrane and Regulate Abscisic
Acid Sensitivity in Arabidopsis
Lesia
Rodriguez, Miguel Gonzalez-Guzman, Maira Diaz, Americo Rodrigues, Ana
C. Izquierdo-Garcia, Marta Peirats-Llobet, Maria A. Fernandez, Regina
Antoni, Daniel Fernandez, Jose A. Marquez, Jose M. Mulet, Armando
Albert, and Pedro L. Rodriguez The
Plant Cell (2014) 26, 4802-4820 (doi:10.1105/tpc.114.129973) Structural Basis for Selective
Recognition of Endogenous and Microbial Polysaccharides by Macrophage
Receptor SIGN-R1
Noella Silva-Martín, Sergio G. Bartual, Erney
Ramírez-Aportela, Pablo Chacón, Chae Gyu Park and
Juan A.
Hermoso Structure
(2014) 22, 1595-1606 (doi:10.1016/j.str.2014.09.001)
Structural
basis of the regulatory mechanism of the plant CIPK family of protein
kinases controlling ion homeostasis and abiotic stress
Antonio Chaves-Sanjuan, Maria Jose Sanchez-Barrena, Juana Maria
Gonzalez-Rubio, Maria Moreno, Paula Ragel, Marta Jimenez, Jose M.
Pardo, Martin Martinez-Ripoll, Francisco J. Quintero, and Armando Albert Proceedings
of the National Academy of Sciences, PNAS (2014) 111, 4532-4541 (doi:10.1073/pnas.1407610111)
Structural
analysis of glucuronoxylan specific Xyn30D and its attached CBM35
domain give insights into the role of modularity in specificity
Sainz-Polo, M.A.Valenzuela, S., González-Pérez,
B., Pastor, F.J., Sanz-Aparicio, J. Journal
of Biological Chemistry (2014) 289, 31088-31101 (doi:
10.1074/jbc.M114.597732)
Crystallization and preliminary
X-ray diffraction data of β-galactosidase from Aspergillus niger Rico-Díaz, A., Vizoso-Vázquez,
A-, Cerdán, M.A., Becerra, M., Sanz-Aparicio, J. Acta Crystallographica (2014)
F70, 1529-1531 (doi:10.1107/S2053230X14019815) Crystallization and preliminary X-ray diffraction
analysis of Xyn30D from Paenibacillus
barcinonensis Sainz-Polo, M.A.Valenzuela, S., Pastor, F.J.,
Sanz-Aparicio, J. Acta
Crystallographica (2014) 963-966 (doi:10.1107/S2053230X14012035)
The guanine-exchange factor Ric8a binds the calcium sensor NCS-1 to
regulate synapse number and probability of release
Jesús Romero-Pozuelo, Jeffrey S. Dason, Alicia Mansilla,
Soledad
Baños-Mateos, José L. Sardina, Antonio
Chaves-Sanjuán, Jaime Jurado-Gómez, Elena
Santana, Harold
L. Atwood, Ángel Hernández-Hernández,
María-José Sánchez-Barrena and Alberto
Ferrús Journal
of Cell Science (2014) 127, 4246-4259(doi:10.1242/
?jcs.152603)
Crystal structures of Ophiostoma piceae
sterol esterase: Activation mechanism and product release
Javier Gutiérrez-Fernández; María
Eugenia Vaquero;
Alicia Prieto; Jorge Barriuso; María Jesús
Martínez and Juan A. Hermoso Journal
of Structural Biology (2014) 187, 215-222(doi:10.1016/j.jsb.2014.07.007)
Structure
of the pneumococcal L,D-carboxypeptidase DacB and pathophysiological
effects of disabled cell wall hydrolases DacA and DacB
Mohammed R.
Abdullah, Javier Gutiérrez-Fernández, Thomas
Pribyl, Nicolas Gisch,
Malek Saleh, Manfred Rohde, Lothar Petruschka, Gerhard Burchhardt,
Dominik Schwudke, Juan A. Hermoso and Sven Hammerschmidt Molecular
Microbiology (2014) 93, 1183–1206 (doi:10.1111/mmi.12729)
Comparative
study of two GH19 chitinase-like proteins from Hevea brasiliensis, one
exhibiting a novel carbohydrate-binding domain
Martínez-Caballero,
Siseth; Cano-Sánchez, Patricia; Mares-Mejía,
Israel; Díaz-Sánchez,
Angel; Macías-Rubalcava, Martha; Hermoso, Juan;
Rodríguez-Romero, Adela FEBS
Journal (2014) 281, 4535–4554(doi:10.1111/febs.12962)
The
consensus-based approach for gene/enzyme replacement therapies and
crystallization strategies: the case of human alanine:glyoxylate
aminotransferase
Mesa-Torres N, Yunta C, Fabelo-Rosa I, Gonzalez-Rubio JM, Sanchez-Ruiz
JM, Salido E, Albert A, Pey AL Biochemical
Journal (2014) 462, 453-463(doi:10.1042/BJ20140250)
Penicillin-binding Protein 2a
of Methicillin-resistant Staphylococcus aureus Jennifer Fishovitz, Juan A
Hermoso, Mayland Chang, and Shahriar Mobashery International
Union of Biochemistry and Molecular Biology Journal (IUBMB Life) (2014)
66, 572-577(doi:10.1002/iub.1289)
Structure and Cell Wall
Cleavage by Modular Lytic Transglycosylase MltC of Escherichia coli C.
Artola-Recolons; M. Lee; N. Bernardo-García; B.
Blázquez;
D. Hesek; S. Bartual; K.V. Mahasenan; E. Lastochkin; H. Pi; K. Meindl;
W. Boggess; I. Uson; J.F. Fisher; S. Mobashery and J.A. Hermoso ACS
Chemical Biology (2014) 9, 2058–2066 (doi:10.1021/cb500439c)
Disruption
of Allosteric Response as an Unprecedented Mechanism of Resistance to
Antibiotics Jennifer
Fishovitz, Alzoray Rojas-Altuve, Lisandro H Otero, Matthew Dawley,
Cesar Carrasco-López, Mayland Chang, Juan A Hermoso and
Shahriar
Mobashery Journal
of the American Chemical Society (2014) 136,
9814–9817 (doi:10.1021/ja5030657)
Structure
solution with ARCIMBOLDO using fragments derived from distant homology
models Sammito,
Massimo; Meindl, Kathrin; de Ilarduya, Iñaki;
Millán,
Claudia; Artola-Recolons, Cecilia; Hermoso, Juan and Usón,
Isabel FEBS
Journal (2014) 281, 4029–4045(doi:10.1111/febs.12897)
Structural
insights into the coenzyme mediated monomer-dimer transition of the
pro-apoptotic Apoptosis Inducing Factor Ferreira,
Patricia; Villanueva, Raquel; Martínez-Júlvez,
Marta;
Herguedas, Beatriz; Marcuello, Carlos; Fernandez-Silva, Patricio;
Cabon, Lauriane; Hermoso, Juan; Lostao, Anabel; Susin, Santos; Medina,
Milagros Biochemistry
(2014) 53, 4204–4215 (doi:10.1021/bi500343r) El CSIC cumple 75
años
Martín Martínez-Ripoll, J. M. López
Sancho, Esteban Moreno Gómez Revista
Española de Física (2014) 28, 25-27 (web-link)[PDF
copy] Aportaciones
de la cristalografía a la medicina. De la
comprensión de las armas
moleculares de los patógenos al desarrollo de
fármacos contra
enfermedades infecciosas
Juan A. Hermoso Investigación
y Ciencia (2014) 454, 11-14 [PDF
copy]
Escherichia coli LacZ
β-galactosidase inhibition by monohydroxyacetylated
glycopyranosides: Role of the acetyl groups
Jana Brabcova, Cesar Carrasco-Lopez, Teodora Bavaro, Juan A. Hermoso,
Jose M. Palomo Journal
of Molecular Catalysis B: Enzymatic (2014) 107, 31-38(doi:10.1016/j.molcatb.2014.05.008)
Tomato
PYR/PYL/RCAR abscisic acid receptors show high expression in root,
differential sensitivity to the abscisic acid agonist quinabactin, and
the capability to enhance plant drought resistance
Gonzalez-Guzman M, Rodriguez L, Lorenzo-Orts L, Pons C,
Sarrion-Perdigones A, Fernandez MA, Peirats-Llobet M, Forment J,
Moreno-Alvero M, Cutler SR, Albert A, Granell A, Rodriguez PL Journal
of Experimental Botany (2014)65, 4451-4464(doi:10.1093/jxb/eru219)
A complement to the modern crystallographer’s toolbox: caged
gadolinium complexes with versatile binding modes
Meike Stelter, Rafael Molina, Sandra Jeudy, Richard Kahn, Chantal
Abergele and Juan A. Hermoso Acta
Crystallographica (2014) D70, 1506–1516 (doi:10.1107/S1399004714005483)
Structural
basis of PcsB-mediated cell separation in Streptococcus pneumoniae
Sergio G. Bartual, Daniel Straume, Gro Anita Stamsås,
Inés
G. Muñoz, Carlos Alfonso, Martín
Martínez-Ripoll,
Leiv Sigve Håvarstein and Juan A. Hermoso Nature
Communications (2014) 5, Article number 3842 (doi:10.1038/ncomms4842)
Peptidoglycan Remodeling by the Coordinated Action of Multispecific
Enzymes
Laura Alvarez, Akbar Espaillat, Juan A. Hermoso, Miguel A. de Pedro,
and Felipe Cava Microbial
Drug Resistance (2014) 20, 1-9(doi:10.1089/mdr.2014.0047)
Frq2
from Drosophila melanogaster: cloning, expression, purification,
crystallization and preliminary X-ray analysis
Baños-Mateos S, Chaves-Sanjuán A, Mansilla A,
Ferrús A, Sánchez-Barrena MJ Acta
Crystallographica (2014) F70, 530-534(doi:10.1107/S2053230X14005408)
Preliminary crystallographic analysis of the ankyrin-repeat domain of Arabidopsis thaliana
AKT1: identification of the domain boundaries for protein
crystallization
Chaves-Sanjuán A, Sánchez-Barrena MJ,
González-Rubio JM, Albert A. Acta
Crystallographica (2014) F70, 509-512(doi:10.1107/S2053230X14005093)
A
través del cristal. Cómo la
cristalografía ha cambiado la visión del mundo Martín
Martínez-Ripoll, Juan A. Hermoso y Armando Albert (coord.) Editorial
CSIC-Catarata (2014)ISBN:
978-84-00-09800-1. Una
historia con claroscuros plagada de laureados Nobel
Martín Martínez-Ripoll in A
través del cristal. Cómo la
cristalografía ha cambiado la visión del mundo
(2014). (M.
Martínez-Ripoll, J.A. Hermoso y Armando Albert, coord.), CSIC-Catarata, pp. 15-41, ISBN:
978-84-00-09800-1.
Una
ventana hacia las enfermedades infecciosas Noelia
Bernardo-García y Juan A. Hermoso in A
través del cristal. Cómo la
cristalografía ha cambiado la visión del mundo
(2014). (M.
Martínez-Ripoll, J.A. Hermoso y Armando Albert, coord.), CSIC-Catarata, pp.
99-118, ISBN:
978-84-00-09800-1. El
arma secreta de la biotecnología
Julia Sanz Aparicio in A
través del cristal. Cómo la
cristalografía ha cambiado la visión del mundo
(2014). (M.
Martínez-Ripoll, J.A. Hermoso y Armando Albert, coord.), CSIC-Catarata, pp.
155-170, ISBN:
978-84-00-09800-1.
Comunicación celular a escala atómica ">
Armando Albert in A
través del cristal. Cómo la
cristalografía ha cambiado la visión del mundo
(2014). (M.
Martínez-Ripoll, J.A. Hermoso y Armando Albert, coord.), CSIC-Catarata, pp. 171-182, ISBN:
978-84-00-09800-1.
A través del cristal y más allá Martín
Martínez-Ripoll, Juan A. Hermoso y Armando Albert in A
través del cristal. Cómo la
cristalografía ha cambiado la visión del mundo
(2014). (M.
Martínez-Ripoll, J.A. Hermoso y Armando Albert, coord.), CSIC-Catarata, pp.
183-188, ISBN:
978-84-00-09800-1.
Esterase
LpEst1 from Lactobacillus
plantarum: A Novel and Atypical Member of the
αβ Hydrolase Superfamily of Enzymes
Yanaisis Alvarez, María Esteban-Torres, Álvaro
Cortés-Cabrera, Federico Gago, Iván
Acebrón,
Rocío Benavente, Karin Mardo, Blanca de las Rivas, Rosario
Muñoz, José M. Mancheño PLoS
ONE (2014) 9: e92257 (doi:10.1371/journal.pone.0092257)
Pneumococcal phosphoglycerate kinase interacts with plasminogen and its
tissue activator M. Fulde, N.
Bernardo-García, M. Rohde, N.
Nachtigall, R. Frank, K.T. Preissner, J. Klett, A. Morreale, G.S.
Chhatwal, J.A. Hermoso, S. Bergmann Thrombosis
and Haemostasis (2014) 111, 401-416 (doi:10.1160/TH13-05-0421)
A
single
mutation in the GSTe2 gene allows tracking of metabolically based
insecticide resistance in a major malaria vector
Riveron JM, Yunta C, Ibrahim SS, Djouaka R, Irving H, Menze BD, Ismail
HM, Hemingway J, H. Ranson, A. Albert and C.S. Wondji Genome Biology (2014) 15, R27
(doi:10.1186/gb-2014-15-2-r27)
Getting
CAD in Shape: The Atomic Structure of Human Dihydroorotase Domain Hermoso J.A. Structure
(2014) 22, 179-181(doi:10.1016/j.str.2014.01.005)
Crystal
structures of CbpF complexed with atropine and ipratropium
reveal clues for the design of novel antimicrobials against Streptococcus pneumoniae
Noella Silva-Martín, Gracia Retamosa; Beatriz Maestro;
Sergio G
Bartual, María J Rodes; Pedro García,
Jesús M
Sanz, Juan A. Hermoso Biochimica
et Biophysica Acta - General Subjects (2014) 1840, 129-135 (doi:10.1016/j.bbagen.2013.09.006)
The
C-terminal extension of bacterial flavodoxin-reductases: involvement in
the hydride transfer mechanism from the coenzyme
Ana Bortolotti, Ana Sánchez-Azqueta, Celia M Maya,
Adrián
Velázquez, Juan A Hermoso, Milagros Medina,
Néstor Cortez Biochimica
et Biophysica Acta - Bioenergetics (2014) 1837, 33-43 (doi:10.1016/j.bbabio.2013.08.008)
Structural
bases for the broad specificity of a new family of amino acid racemases
Akbar Espaillat, Cesar
Carrasco-López, Noelia
Bernardo-García, Natalia
Pietrosemolli, Lisandro H. Otero, Laura
Alvarez, Miguel
A. de Pedro, Florencio Pazos, Brigid
M. Davis, Matthew K. Waldor, Juan
A. Hermoso and Felipe Cava Acta
Crystallographica (2014) D70, 79-90 (doi:10.1107/S1399004713024838) 2013
Molecular
architecture of Streptococcus
pneumoniae surface thioredoxin-fold lipoproteins crucial
for extracellular oxidative stress resistance and maintenance of
virulence
Malek Saleh, Sergio G. Bartual, Mohammed R. Abdullah, Inga Jensch,
Tauseef M. Asmat, Lothar Petruschka, Thomas Pribyl, Juan A. Hermoso and
Sven Hammerschmidt EMBO
Molecular Medicine (2013) 5, 1852-1870 (doi:10.1002/emmm.201202435)
The role of protein denaturation energetics
and molecular chaperones in the aggregation and mistargeting of mutants
causing primary hyperoxaluria type I
Mesa-Torres, N., Fabelo-Rosa, I., Riverol, D., Yunta, C., Albert, A.,
Salido, E., Pey, A.L. PLoS
ONE (2013) 8, e71963(doi:10.1371/journal.pone.0071963)
Evolutionary
Adaptation of the Fly Pygo PHD Finger toward Recognizing Histone H3
Tail Methylated at Arginine 2
Thomas C.R. Miller, Juliusz Mieszczanek, María
José
Sánchez-Barrena, Trevor J. Rutherford, Marc Fiedlersend,
Mariann
Bienz Structure
(2013) 21, 2208–2220
(doi:10.1016/j.str.2013.09.013)
Characterization
of a feruloyl esterase from Lactobacillus
plantarum.
Esteban-Torres M, Reverón I, Mancheño JM, de Las
Rivas B, Muñoz R. Applied
and Environmental Microbiology (2013) 79, 5130-5136(doi:10.1128/AEM.01523-13)
Identification of a missing link in the evolution of an enzyme into a
transcriptional regulator.
Durante-Rodríguez G, Mancheño JM, Rivas G,
Alfonso C, García JL, Díaz E, Carmona M. PLoS
ONE (2013) 8, e57518(doi:10.1371/journal.pone.0057518)
The PYL4 A194T mutant uncovers a key role of
PYR1-LIKE4/PROTEIN PHOSPHATASE 2CA interaction for abscisic acid
signaling and plant drought resistance
Pizzio, G.A., Rodriguez, L., Antoni, R., González-Guzman,
M.,
Yunta, C., Merilo, E., Kollist, H., Albert, A., Rodriguez, P.L. Plant
Physiology (2013) 163, 441-55 (doi:
10.1104/pp.113.224162)
Structure, biochemical
characterization and analysis of the pleomorphism of carboxylesterase
Cest-2923 from Lactobacillus
plantarum WCFS1
Benavente, R; Esteban-Torres, M; Acebrón, I; de Las Rivas,
B; Muñoz, R; Alvarez, Y; Mancheño, JM. The
FEBS Journal (2013) 280, 6658-6671(doi:10.1111/febs.12569)
How allosteric
control of Staphylococcus
aureus penicillin binding protein 2a enables methicillin
resistance and
physiological function Lisandro H. Otero, Alzoray
Rojas-Altuve, Leticia I. Llarrull, Cesar Carrasco-López,
Malika Kumarasiri, Elena Lastochkin, Jennifer Fishovitz, Matthew
Dawley, Dusan Hesek, Mijoon Lee, Jarrod W. Johnson, Jed F. Fisher,
Mayland Chang, Shahriar Mobashery, Juan A. Hermoso Proceedings
of the National Academy of Sciences (2013) 110,
16808-16813(doi:10.1073/pnas.1300118110)
Cell-Wall
Remodeling by the Zinc-Protease AmpDh3 from Pseudomonas aeruginosa
Lee, M., Artola-Recolons, C., Carrasco-López, C.,
Martínez-Caballero, S., Hesek, D., Spink, E., Lastochkin,
E.,
Zhang, W., Hellman, L., Boggess, B., Hermoso, J. and Mobashery, S. Journal
of the American Chemical Society (2013) 135,
12604-12607 (doi:10.1021/ja407445x)
Protein homeostasis defects of alanine-glyoxylate aminotransferase: new
therapeutic strategies in primary hyperoxaluria type I
Pey, A.L., Albert, A., Salido, E. Biomed
Research International (2013) 2013:687658 (doi:10.1155/2013/687658)
Reaction
products and the X-ray structure of AmpDh2, a virulence determinant of Pseudomonas aeruginosa Siseth
Martínez-Caballero, Mijoon Lee, Cecilia Artola-Recolons,
César
Carrasco-López, Dusan Hesek, Edward Spink, Elena Lastochkin,
Weilie
Zhang, Lance M. Hellman, Bill Boggess, Shahriar Mobashery and Juan A.
Hermoso Journal
of the American Chemical Society (2013) 135, 10318−10321
(doi:10.1021/ja405464b)
¿Cómo aprendimos a "ver" los átomos?
Martín Martínez-Ripoll in Serie El CSIC
en la Escuela, Investigación sobre la enseñanza
de la ciencia en el aula (2013), Number 9, pp.
9-34, e-ISBN (n.º 9): 978-84-00-09659-5 [PDF-copy]
Crystal
structure and functional insights into uracil-DNA glycosylase
inhibition by phage ?29 DNA mimic protein p56 José
Ignacio Baños-Sanz, Laura Mojardín, Julia
Sanz-Aparicio, José M.
Lázaro, Laurentino Villar, Gemma Serrano-Heras, Beatriz
González, and
Margarita Salas Nucleic
Acids Research (2013) 41,
6761-6773 (doi:10.1093/nar/gkt395)
Heteroresistance
to Fosfomycin Is Predominant in Streptococcus
pneumoniae and Depends on the murA1 Gene
Hansjürg
Engel, Javier Gutiérrez-Fernández, Christine
Flückiger, Martín
Martínez-Ripoll, Kathrin Mühlemann, Juan A.
Hermoso, Markus Hilty and
Lucy J. Hathaway Antimicrobial
Agents and Chemotherapy (2013) 57, 2801-2808(doi:10.1128/AAC.00223-13)
Structural Biology of a Major Signaling Network that Regulates Plant
Abiotic Stress: The CBL-CIPK Mediated Pathway María
José Sánchez-Barrena, Martín
Martínez-Ripoll and Armando Albert International
Journal of Molecular Sciences (2013) 14, 5734-5749(doi:10.3390/ijms14035734)
Synthesis
of 6-Kestose using an Efficient β-Fructofuranosidase
Engineered by Directed Evolution
Miguel de Abreu, Miguel Alvaro-Benito, Julia Sanz-Aparicio, Francisco
J. Plou, Maria Fernandez-Lobato and Miguel Alcalde Advanced
Synthesis & Catalysis (2013) 355,
1698–1702 (doi:10.1002/adsc.201200769)
The three-dimensional structure of Saccharomyces
invertase: role of a noncatalytic domain in oligomerization and
substrate specificity
M. Angela Sainz-Polo, Mercedes Ramírez-Escudero, Alvaro
Lafraya, Beatriz González, Julia Marín-Navarro,
Julio Polaina and Julia Sanz-Aparicio Journal
of Biological Chemistry (2013) 288, 9755-9766(doi:10.1074/jbc.M112.446435)
Highly functionalized
β,γ–diamino compounds through reductive
amination of
amino acid-derived β–keto esters
P.
Pérez-Faginas, M.T. Aranda, M.T.
García-López, L. Infantes, A.
Fernández-Carvajal, J.M. González-Ros, A.
Ferrer-Montiel and R.
González-Muñiz PLoS
ONE (2013) 8, e53231(doi:10.1371/journal.pone.0053231)
The Silver-catalyzed Cyclization of N-(prop-2-yn-1-yl)pyridin-2-amines
M. Chioua, E. Soriano, L. Infantes, M.L. Jimeno, J. Marco-Contelles and
A. Samadi European
Journal of Organic Chemistry (2013) 1, 35-39(doi:10.1002/ejoc.201201258)
Structural
and Phylogenetic Analysis of Rhodobacter
capsulatus NifF:
Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins
Pérez-Dorado I., Bortolotti A., Cortez N., Hermoso J.A. International
Journal of Molecular Sciences (2013) 14, 1152-1163 (doi:10.3390/ijms14011152)
Sulfoxide-Directed
Enantioselective Synthesis of Functionalized Tetrahydropyridines
Fernández de la Pradilla, R.; Simal, C.; Bates, R.H.; Viso,
A.; Infantes, L. Organic
Letters (2013) 15, 4936-4939(doi:10.1021/ol402141d)
Efficient Light Harvesters based on the
10-(1,3-Dithiol-2-ylidene)anthracene core
Bouit, P.A.; Infantes, L.; Calbo, J.; Viruela, R.; Ortí, E.;
Delgado, J.L.; Martín, N. Organic
Letters (2013) 15, 4166-4169 (doi:10.1021/ol401841u)
The
Structure and Dynamic Properties of 1H-Pyrazole-4-carboxylic acids in
the Solid State
Infantes, L.; García, M.A.; López, C.; Claramunt,
R.M.; Elguero, J. Zeitschrift
für Physikalische Chemie (2013) 227, 841-856(doi:10.1524/zpch.2013.0377) 2012
Bin2 Is a
Membrane Sculpting N-BAR Protein That Influences Leucocyte Podosomes,
Motility and Phagocytosis
Sánchez-Barrena, M.J., Vallis,
Y., Clatworthy,
M.R., Doherty, G.J., Veprintsev,
D.B., Evans, P.R. and
McMahon, H.T. PLoS
One (2012) 7(12), e52401 (doi:10.1371/journal.pone.0052401)
NADP+
Binding to the Regulatory Subunit of
Methionine Adenosyltransferase II Increases Intersubunit Binding
Affinity in the Hetero-Trimer Gonzalez, B;
Garrido, F; Ortega,
R; Martínez-Julvez, M; Revilla-Guarinos, A;
Pérez-Pertejos. Y; Velázquez-Campoy, A;
Sanz-Aparicio, J;
and Pajares, M PLoS
ONE (2012) 7(11), e50329 (doi:10.1371/journal.pone.0050329)
¿Cómo aprendimos a “ver”
átomos y moléculas después de von Laue?
Martín Martínez-Ripoll y Pascual
Román-Polo Anales
de Química (2012) 108(3), 225–235 [PDF-copy]
Azepane quaternary amino acids as effective inducers
of 310 helix conformations
D. Nuñez-Villanueva, L. Infantes, M.T. Garcia-Lopez, R.
Gonzalez-Muñiz, M. Martín-Martínez Journal
of Organic Chemistry (2012) 77, 9833-9839 (doi:10.1021/jo301379r) Structural insights on the plant
Salt-Overly-Sensitive 1 (SOS1) Na+/H+
antiporter
Rafael Núñez-Ramírez, María
José
Sánchez-Barrena, Irene Villalta, Juan F. Vega, Jose M.
Pardo,
Francisco J. Quintero, Javier Martinez-Salazar and Armando Albert Journal
of Molecular Biology (2012) 424, 283-294 (doi:10.1016/j.jmb.2012.09.015)
The Structural
Domains of Pseudomonas
aeruginosa Phosphorylcholine Phosphatase Cooperate in
Substrate Hydrolysis: 3D Structure and Enzymatic Mechanism
Lourdes Infantes, Lisandro Horacio Otero, Paola Rita Beassoni,
Cristhian Boetsch, Angela Teresita Lisa, Carlos Eduardo Domenech and
Armando Albert Journal of
Molecular Biology (2012) 423, 503–514 (doi:10.1016/j.jmb.2012.07.024)
Semisynthetic
peptide-lipase conjugates for improved biotransformations
O. Romero, M. Filice, B. de las Rivas, C. Carrasco-Lopez, J. Klett, A.
Morreale, J.A. Hermoso, J.M. Guisan, O. Abian and J.M. Palomo Chemical
Communications (2012) 48, 9053-9055(doi:10.1039/C2CC34816K)
Conformational
Changes Undergone by Inositol
1,3,4,5,6-Pentakisphosphate 2-Kinase Upon Substrate Binding: The Role
of N-lobe and Enantiomeric Substrate Preference
Jose Ignacio Baños-Sanz, Julia Sanz-Aparicio, Hayley
Whitfield, Chris Hamilton, Charles A. Brearley and Beatriz Gonzalez Journal
of Biological Chemistry (2012) 287, 29237-29249(doi:10.1074/jbc.M112.363671)
Pneumococcal
surface proteins: when the whole is greater than the sum of its parts
I. Pérez-Dorado, S. Galán-Bartual and J.A. Hermoso Molecular
Oral Microbiology (2012) 27, 221–245
(invited review) (doi:10.1111/j.2041-1014.2012.00655.x)
Crystallization and preliminary X-ray
diffraction analysis of the invertase from Saccharomyces cerevisiae M. A. Sainz-Polo, A. Lafraya, A. Polo, J.
Marín-Navarro, J. Polaina and J. Sanz-Aparicio Acta
Crystallographica (2012) F68, 1538-1541 (doi:10.1107/S1744309112044417)
Expression,
purification, crystallization and preliminary X-ray diffraction
analysis of the apo form of InsP5 2-K from
Arabidopsis thaliana
J.I. Baños-Sanz, J. Sanz-Aparicio, C.A.
Brearley and B. González Acta
Crystallographica (2012) F68, 701-704 (doi:10.1107/S1744309112017307)
Structural and kinetic insights reveal that the amino acid pair
GLN228/ASN254 modulates the transfructosylating specificity of Schwanniomyces occidentalis
β-fructofuranosidase, an enzyme that produces prebiotics
Miguel Alvaro-Benito, M. Angela Sainz-Polo, David Gonzalez-Perez,
Beatriz Gonzalez, Francisco J. Plou, Maria Fernandez-Lobato, and Julia
Sanz Aparicio Journal
of Biological Chemistry (2012) 287, 19674-19686(doi:10.1074/jbc.M112.355503)
An analysis of subdomain orientation, conformational change and
disorder in relation to crystal packing of aspartic proteinases
D. Bailey, E.P. Carpenter, A. Coker, S. Coker, J. Read, A.T. Jones, P.
Erskine, C.F. Aguilar, M. Badasso, L. Toldo, F. Rippmann, J.
Sanz-Aparicio, A. Albert, T.L. Blundell, N.B., Roberts, S.P. Wood and
J.B. Cooper Acta
Crystallographica (2012) D68, 541-552(doi:
10.1107/S0907444912004817)
Structural
basis of specificity in tetrameric Kluyveromyces
lactis β-galactosidase
Pereira-Rodríguez, A; Fernández-Leiro,
R; González-Siso, MI; Esperanza
Cerdán,
M; Becerra, M; Sanz-Aparicio, J Journal
of Structural Biology (2012) 177, 392–401 (doi:10.1016/j.jsb.2011.11.031)
Promiscuous
enantioselective (–)-γ-lactamase
activity in the Pseudomonas fluorescens esterase I
Leticia Luciana Torres, Marlen Schmidt, Anna Schliessmann, Noella
Silva-Martin, Juan Hermoso, José Berenguer, Uwe Bornscheuer
and
Aurelio Hidalgo Organic
& Biomolecular Chemistry (2012) 10, 3388-3392(doi:
10.1039/C2OB06887G)
2011 Nobel
de Química 2011 a Daniel Shechtman por su descubrimiento de
los cuasicristales y de una simetría "imposible"
Julia Sanz-Aparicio mi+d
(2011) published in Análisis
Madri+d on 15 Nov. 2011[PDF-copy]
A Practical
Two-Step Synthesis of Imidazo[1,2-a]pyridines from
N-(prop-2-yn-1-yl)pyridin-2-amines D. Sucunza, A. Samadi, M.
Chioua, D.B. Silva, C. Yunta, L. Infantes, M.C. Carreiras, E. Soriano,
J. Marco-Contelles Chemical
Communications (2011) 47, 5043-5045 (doi:10.1039/C1CC10641D)
Quaternary
α,α,-2-Oxoazepane
alpha-Amino Acids: Synthesis from Ornithine-Derived β-Lactams
and
Incorporation into Model Dipeptides D.
Nuñez-Villanueva, M.A. Bonache, L. Infantes, M.T.
Garcia-Lopez, M. Martin-Martinez, R. Gonzalez-Muniz Journal
of Organic Chemistry (2011) 76, 6592-6603(doi:10.1021/jo200894d)
Crystallization and preliminary crystallographic analysis of a C2
protein from Arabidopsis thaliana
Maira Diaz, Lesia Rodriguez, Miguel Gonzalez-Guzman, Martin
Martinez-Ripoll and Armando Albert Acta
Crystallographica (2011) F67, 1575–1578(doi:10.1107/S1744309111040541)
Preliminary
X-ray analysis of twinned crystals of the Q88Y25_Lacpl esterase from Lactobacillus
plantarum WCFS1
Yanaisis
Alvarez, Maria Esteban-Torres, Ivan Acebron, Blanca de las Rivas,
Rosario Muñoz, Martin Martinez-Ripoll and Jose M.
Mancheño Acta
Crystallographica (2011) F67, 1436–1439 (doi:10.1107/S1744309111036682)
The
structure of Arabidopsis
thaliana OST1 provides insights into the
kinase regulation mechanism in response to osmotic stress
Cristina Yunta, Martín Martínez-Ripoll, Jian-Kang
Zhu and Armando Albert Journal
of Molecular Biology (2011) 414, 135–144(doi:10.1016/j.jmb.2011.09.041)
Fructo-oligosaccharide
synthesis by mutant versions of Saccharomyces
cerevisiae invertase
Lafraya, A., Sanz-Aparicio, J., Polaina, J. and
Marín-Navarro, J. Applied
Environmental Microbiology (2011)
77, 6148-6157(doi:10.1128/AEM.05032-11)
Crystallization
and Preliminary X-Ray Diffraction Analysis of phosphoglycerate kinase
from Streptococcus
pneumoniae N.
Bernardo-García, S.G. Bartual, M. Fulde, S. Bergmann and
J.A. Hermoso Acta
Crystallographica (2011) F67, 1285-1289 (doi:10.1107/S1744309111030922)
Crystallization and Preliminary X-Ray
Diffraction Analysis of the transcriptional repressor Paax, the main
regulator of the phenylacetic acid degradation pathway in Escherichia
coli W. A. Rojas-Altuve, C.
Carrasco-López, V.M. Hernández-Rocamora, J.M.
Sanz and J.A. Hermoso Acta
Crystallographica (2011) F67, 1278-1280 (doi:10.1107/S1744309111029873)
Crystal Structures of Bacterial Peptidoglycan Amidase AmpD and an
Unprecedented Activation Mechanism
César Carrasco-Lopez, Alzoray Rojas-Altuve, Welie
Zhang,
Dusan Hesek, Mijoon Lee, Sophie Barbe, Isabelle
André, Pilar Ferrer, Noella Silva-Martín,
Germán
R.
Castro, Martín Martínez-Ripoll, Shahriar
Mobashery, and Juan A. Hermoso Journal
of Biological Chemistry (2011) 286,
31714-31722 (doi:10.1074/jbc.M111.264366)
High-resolution
structural insights on the sugar-recognition and fusion tag properties
of a versatile β-trefoil lectin domain from the mushroom Laetiporus sulphureus
Angulo, I., Acebrón, I., de las Rivas, B., Muñoz,
R., Rodríguez-Crespo, I., Menéndez, M.,
García, P., Tateno, H., Goldstein,
I.J., Pérez-Agote, B. and Mancheño, J.M. Glycobiology
(2011) 21, 1349-1361(doi:10.1093/glycob/cwr074)
An esterase
from Thermus
thermophilus HB27
with hyper-thermoalkalophilic properties: purification,
characterisation and structural modelling Fuciños, P.,
Pastrana, L., Sanromán, A., Longo, M.A., Hermoso, J.A.,
Rúa M.L. Journal
of Molecular Catalysis B: Enzymatic (2011) 70, 127-137 (doi:10.1016/j.molcatb.2011.02.017)
High-Resolution
Crystal
Structure of an Outer Membrane-Anchored Endolytic Peptidoglycan Lytic
Transglycosylase (MltE) from Escherichia
coli
Cecilia Artola-Recolons, Cesar Carrasco-López, Leticia Irene
Llarrull, Malika Kumarasiri, Elena Lastochkin, Iñaki
Martínez de Ilarduya, Kathrin Meindl, Isabel
Usón,
Shahriar Mobashery and Juan A. Hermoso Biochemistry (2011) 50, 2384-2386(doi:10.1021/bi200085y) Coordination
driven or/and H-bonded Cu(II)-N,N-dialkylisonicotinamide frameworks
Ajay
Pal Singh Pannu, Pratibha Kapoor, Geeta Hundal, Ramesh Kapoor, Martin
Martinez-Ripoll, Rayond J. Butcher, Maninder Singh Hundal Polyhedron
(2011) 30, 1691-1702 (doi:10.1016/j.poly.2011.03.047)
A self
assembled 3-D network propagated by
coordination polymerization and H-bonding: synthesis and X-ray crystal
structure of [{Co(L)2(H2O)2}(ClO4)2(CH3COCH3)2(H2O)2]n,
where L = N,N-diisopropylisonicotinamide
Ajay Pal Singh Pannu, Pratibha Kapoor, Geeta Hundal, Ramesh Kapoor,
Martin Martinez-Ripoll and Maninder Singh Hundal Journal
of Coordination Chemistry (2011) 64, 1566-1577(doi:10.1080/00958972.2011.574286)
SnRK2.6/OST1
from Arabidopsis
thaliana: cloning, expression, purification,
crystallization and preliminary X-ray analysis of K50N and D160A mutants Cristina
Yunta, Martin Martinez-Ripoll and Armando Albert Acta
Crystallographica (2011) F67, 364–368(doi:10.1107/S1744309110053807)
Crystallization
and Preliminary X-Ray Diffraction Analysis of Lytic
Transglycosylase MltE from Escherichia
coli Cecilia
Artola-Recolons, Leticia Llarrull, Elena Lastochkin, Shahriar Mobashery
and Juan A. Hermoso Acta
Crystallographica (2011) F67, 161-163 (doi:10.1107/S1744309110049171)
New Alkaloid
Antibiotics that Target the DNA Topoisomerase I of Streptococcus
pneumoniae Teresa
García, María Amparo Blázquez,
María
José Ferrándiz, María
Jesús Sanz, Noella Silva-Martín, Juan
A. Hermoso
and Adela G. de la Campa Journal
of Biological Chemistry (2011) 286, 6402-6413 (doi:10.1074/jbc.M110.148148)
The
pURI family of
expression vectors: A versatile set of ligation
independent cloning plasmids for producing recombinant His-fusion
proteins. Curiel, J.A., de Las Rivas., B,
Mancheño, J.M., Muñoz, R. Protein
Expression and Purification (2011) 76, 44-53 (doi:10.1016/j.pep.2010.10.013) 2010 Crystallographers
in Spain
Martín Martínez-Ripoll IUCr
Newsletter (2010) 18(4), 5-13 [PDF-copy] Crystallography
in Spain
Martín Martínez-Ripoll IUCr
Newsletter (2010) 18(3), 5-9 [PDF-copy]
Cristalografía en España
Martín Martínez-Ripoll Anales
de Química (2010) 106, 319-329 [PDF-copy] Controlling
Optical Properties and Function of BODIPY by Using Asymmetric
Substitution Effects
J.
Bañuelos-Prieto, A.R. Agarrabeitia, I.
García-Moreno, I. López Arbeloa,
A. Costela, L. Infantes, M.E. Perez-Ojeda, M. Palacios-Cuesta and M.J.
Ortiz Chemistry
European Journal (2010) 16, 14094-14105(doi:10.1002/chem.201002095)
Biochemical
characterization of the transcriptional regulator BzdR from Azoarcus
sp. CIB Durante-Rodriguez,
G., Valderrama, A., Mancheño, J.M., Rivas, G., Alfonso, C.,
Arias-Palomo, E., Llorca, O., Garcia, J.L., Diaz, E., Carmona, M. Journal
of Biological Chemistry (2010)
285, 35694-35705 (doi:10.1074/jbc.M110.143503)
Synthesis of
(E)-diethyl
6,60-(diazene-1,2-diyl)bis(5-cyano-2-methyl-4-phenylnicotinates), a new
type of 2,20-azopyridine dyesodification of pancreatic lipase
properties by directed molecular evolution Botelho
da Silva, D., Samadi, A., Infantes, L., Carreiras, M.C. and
Marco-Contelles, J. Tetrahedron
Letters (2010) 51, 6278-6281 (doi:10.1016/j.tetlet.2010.09.095)
Modification of pancreatic lipase properties by directed molecular
evolution Colin, D.Y.,
Deprez-Beauclair, P., Silva, N., Infantes, L. and
Kerfelec, B. Protein
Engineering Design & Selection (2010) 23, 365-373 (doi:10.1093/protein/gzq008) New
insights
into the fructosyltransferase
activity of Schwanniomyces occidentalis β-fructofuranosidase,
emerging
from nonconventional codon usage and directed mutation Alvaro-Benito, M., Abreu,
M., Portillo, F., Sanz-Aparicio, J. and Fernández-Lobato, M. Applied
Environmental Microbiology (2010) 76, 7491-7499(doi:10.1128/AEM.01614-10)
Crystallization
and preliminary X-ray diffraction analysis of the fructofuranosidase
from Xanthophyllomyces
dendrorhous Polo, A.,
Linde, D.,
Estévez, M., Fernández-Lobato, M. and
Sanz-Aparicio, J. Acta
Crystallographica (2010) F66, 1441-1444(doi:10.1107/S1744309110029192)
Crystallization
and
preliminary X-ray diffraction analysis of Pseudomonas aeruginosa
phosphorylcholine phosphatase L.H. Otero, P.R.
Beassoni, C.E. Domenech, A.T. Lisa and A. Albert Acta
Crystallographica (2010) F66, 957-960 (doi:10.1107/S1744309110024061)
Structural
analysis of Saccharomyces
cerevisiae
α-galactosidase and its complexes with natural substrates
reveals
new insights into substrate specificity of GH27 glycosidases
Rafael Fernández-Leiro, Ángel
Pereira-Rodríguez,
M. Esperanza Cerdán, Manuel Becerra and Juliana Sanz-Aparicio Journal
of Biological Chemistry (2010) 285, 28020-28033 (doi:10.1074/jbc.M110.144584)
Inositol
1,3,4,5,6-pentakisphosphate 2-kinase
is a distant IPK member with a singular inositide binding site for
axial 2-OH recognition B. González, J.
Baños-Sanz, M. Villate, C. Brearley and J. Sanz-Aparicio Proceedings
of the National Academy of Sciences (2010) 107,
9608-9613 (doi:10.1073/pnas.0912979107) Insights
into
pneumococcal fratricide from crystal structure of the modular Killing
Factor LytC
Inmaculada Pérez-Dorado, Ana González,
María
Morales, Reyes Sanles, Waldemar Striker, Waldemar Vollmer, Shahriar
Mobashery, José L. García, Martín
Martínez-Ripoll, Pedro García and Juan A. Hermoso Nature
Structural & Molecular Biology (2010) 17, 576-582 (doi:10.1038/nsmb.1817) Promotion of
multipoint covalent
immobilization through different regions of genetically modified
penicillin G acylase from E.
coli Grazú V.,
López-Gallego
F., Montes T., Abian O., González R., Hermoso J.A.,
García J.L., Mateo C. and Guisán J.M. Process
Biochemistry (2010) 45, 390-398 (doi:10.1016/j.procbio.2009.10.013)
Pneumococcal
CbpD is a murein hydrolase that
requires a dual cell envelope binding specificity to kill target cells
during fratricide
Vegard Eldholm, Ola Johnsborg, Daniel Straume, Hilde Solheim Ohnstad,
Kari Helene Berg, Juan A. Hermoso and Leiv Sigve Håvarstein Molecular
Microbiology (2010) 76, 905-917
(doi:10.1111/j.1365-2958.2010.07143.x) Crystallization
and preliminary crystallographic analysis of the catalytic module of
endolysin from Cp-7, a phage infecting Streptococcus pneumoniae Noella Silva-Martin, Rafael Molina, Ivan Angulo,
José M. Mancheño, Pedro García and
Juan A. Hermoso Acta
Crystallographica (2010) F66, 670–673 (doi:10.1107/S1744309110006718)
Crystallization
of the pneumococcal autolysin LytC: in-house phasing using novel
lanthanide complexes
I. Pérez-Dorado, R. Sanles, A. González, P.
García, J.L. García, M.
Martínez-Ripoll and J.A.
Hermoso Acta
Crystallographica (2010) F66, 448-451 (doi:10.1107/S1744309110006081)
Oligomeric
State in the Crystal Structure of
Modular FAD Synthetase Provides Insights into Its Sequential Catalysis
in Prokaryotes
Beatriz Herguedas, Marta Martínez-Júlvez, Susana
Frago, Milagros Medina and Juan A. Hermoso Journal
of Molecular Biology (2010) 400, 218–230 (doi:10.1016/j.jmb.2010.05.018)
p-Coumaric acid decarboxylase from
Lactobacillus plantarum: Structural insights into the active site and
decarboxylation catalytic mechanism
Héctor Rodríguez, Iván Angulo, Blanca
de las
Rivas, Nuria Campillo, Juan A. Páez, Rosario
Muñoz and
José M. Mancheño Proteins:
Structure, Function, and Bioinformatics (2010)78, 1662-1676 (doi:10.1002/prot.22684)
Laetiporus
sulphureus Lectin and
Aerolysin Protein Family
Jose Miguel Mancheño, Hiroaki Tateno, Daniel Sher and Irwin
J. Goldstein
in Proteins:
Membrane Binding and Pore Formation(2010),
Chp. 6, pp. 67-80.
Gregor Anderluh and Jeremy Lakey, Eds.,
Landes Bioscience. ISBN:
978-1-4419-6326-0. See also Advances in
Experimental Medicine and Biology (2010) 677, 67-80
Structural and
kinetic analysis of Schwanniomyces
occidentalis invertase reveals a new oligomerization
pattern and the role of its supplementary domain in substrate binding Alvaro-Benito, M., Polo,
A., González, B., Fernández-Lobato, M. and
Sanz-Aparicio, J. Journal
of Biological Chemistry (2010)285,
13930-13941
(doi:10.1074/jbc.M109.095430)
Structure of GroEL in Complex with an Early Folding Intermediate of
Alanine Glyoxylate Aminotransferase
Armando Albert, Cristina Yunta, Rocío Arranz,
Álvaro
Peña, Eduardo Salido, José María
Valpuesta and
Jaime Martín-Benito Journal
of Biological Chemistry (2010) 285, 6371-6376 (doi:10.1074/jbc.M109.062471)
Crystallization
and preliminary X-ray crystallographic analysis of
β-galactosidase from Kluyveromyces
lactis Pereira-Rodríguez,
A. Fernández-Leiro, R., González-Siso, M.,
Cerdán, E., Becerra, M. and Sanz-Aparicio, J. Acta
Crystallographica (2010) F66, 297-300(doi:10.1107/S1744309109054931)
Crystallization
and preliminary X-ray
diffraction analysis of inositol 1,3,4,5,6-pentakisphosphate kinase
from Arabidopsis
thaliana
Baños-Sanz, J.I., Villate, M., Sanz-Aparicio, J., Brearley,
C.A. and González B. Acta
Crystallographica (2010) F66, 102-106(doi:10.1107/S1744309109051057)
Crystallization
and preliminary X-ray diffraction analysis of α-galactosidase
from Saccharomyces
cerevisiae Fernández-Leiro,
F., Pereira, A., Cedán, E. Becerra-Fernández, M.
and Sanz-Aparicio, J. Acta
Crystallographica (2010) F66, 44-47 (doi:10.1107/S1744309109047794)
Structural insights into the specificity of Xyn10B
from Paenibacillus
barcinonensis and its improved stability by
forced protein evolution
Gallardo, O. Pastor, F.J., Polaina, J., Díaz, P., Vogel, P.
Isorna, P., González-Pérez, B. and Sanz-Aparicio,
J. Journal
of Biological Chemistry (2010) 285, 2721-2733 (doi:10.1074/jbc.M109.064394)
2009 Sticholysins,
two pore-forming toxins produced by the Caribbean Sea anemone Stichodactyla
helianthus: their interaction with membranes
C. Alvarez, J.M. Mancheño, D. Martínez, M.
Tejuca, F. Pazos and M.E. Lanio Toxicon
(2009) 54, 1135-1147 (doi:10.1016/j.toxicon.2009.02.022)
Food phenolics and lactic acid bacteria
H. Rodriguez, J.A. Curiel, J.M. Landete, B. de las Rivas, F.
López de Felipe, C. Gómez-Cordovés,
J.M.
Mancheño and R. Muñoz International
Journal of Food Microbiology (2009) 132, 79-90 (doi:10.1016/j.ijfoodmicro.2009.03.025)
Production and physicochemical properties of recombinant Lactobacillus
plantarum tannase
J.A. Curiel, H. Rodríguez, I. Acebrón, J.M.
Mancheño, B. de Las Rivas and R. Muñoz Journal
of Agricultural and Food Chemistry (2009) 57,
6224-6230
(doi:10.1021/jf901045s)
Crystal structure of the hexameric catabolic ornithine transcarbamylase
from Lactobacillus
hilgardii: Structural insights into the oligomeric
assembly and metal binding
B. de Las Rivas, G.C.
Fox, I.
Angulo, M. Martinez-Ripoll, H. Rodríguez, R.
Muñoz and J.M. Mancheño Journal
of Molecular Biology (2009) 393, 425-434 (doi:10.1016/j.jmb.2009.08.002)
Crystallization and
preliminary X-ray diffraction studies of the FAD synthetase from Corynnebacterium
ammoniagenes Beatriz
Herguedas, Marta Martínez-Júlvez,
Milagros Medina and Juan A. Hermoso Acta
Crystallographica (2009) F65, 1285-1288(doi:10.1107/S1744309109044789)
Crystallization and preliminary X-ray
diffraction studies of the carbohydrate recognition domain of SIGN-R1,
a receptor for microbial polysaccharides and sialylated antibody on
splenic marginal zone macrophages N. Silva-Martin, J.D.
Schauer, C.G. Park and J.A. Hermoso Acta
Crystallographica (2009) F65, 1264-1266 (doi:10.1107/S1744309109041992)
Crystallization
and preliminary X-ray diffraction analysis of the fructofuranosidase
from Schwanniomyces
occidentalis Polo-Rivas, A.
Alvaro-Benito, A., Fernández-Lobato, M. and Sanz-Aparicio, J. Acta
Crystallographica (2009) F65, 1162-1165 (doi:10.1107/S174430910903938)
Discovery of specific flavodoxin inhibitors as potential therapeutic
agents against Helicobacter
pylori infection Nunilo Cremades,
Adrián
Velázquez-Campoy, Marta
Martínez-Júlvez,
José L. Neira, Inmaculada Pérez-Dorado, Juan
Hermoso,
Pilar Jiménez, Angel Lanas, Paul S. Hoffman and Javier Sancho ACS
Chemical Biology (2009) 4, 928-938 (doi:10.1021/cb900166q)
Cloning, production,
purification and
preliminary crystallographic analysis of a glycosidase from the food
lactic acid bacterium Lactobacillus
plantarum CECT 748T Iván
Acebrón, José A. Curiel, Blanca de las Rivas,
Rosario Muñoz, José M. Mancheño Protein
Expression and Purification (2009)68, 177-182(doi:10.1016/j.pep.2009.07.006)
Characterization of
gadolinium complexes for SAD phasing in macromolecular crystallography:
application to CbpF
Rafael Molina, Meike Stelter, Richard Kahn and Juan A. Hermoso Acta
Crystallographica (2009) D65, 823–831 (doi:10.1107/S0907444909017958)
Protein Motifs
Involved in Coenzyme Interaction and Enzymatic Efficiency in Anabaena
Ferredoxin–NADP+ Reductase José
Ramón Peregrina, Beatriz Herguedas, Juan A. Hermoso, Marta
Martínez-Júlvez and Milagros Medina Biochemistry
(2009) 48, 3109-3119 (doi:10.1021/bi802077c)
Crystal structure of CbpF,
a bifunctional choline-binding protein and autolysis regulator from Streptococcus
pneumoniae
Rafael Molina, Ana González, Meike Stelter, Inmaculada
Pérez-Dorado, Richard Kahn, María Morales, Susana
Campuzano, Nuria E. Campillo, Shahriar Mobashery, José L.
García, Pedro García and Juan A. Hermoso EMBO
Reports (2009) 10, 246-251 (doi:10.1038/embor.2008.245) Activation
of bacterial thermoalkalophilic lipases is spurred by dramatic
structural rearrangements
Cesar Carrasco-López, Cesar Godoy, Blanca de las Rivas,
Gloria
Fernandez-Lorente, Jose M. Palomo, José M.
Guisán,
Roberto Fernández-Lafuente, Martín
Martínez-Ripoll
and Juan A. Hermoso Journal
of Biological Chemistry (2009) 284, 4365-4372 (doi:10.1074/jbc.M808268200)
Coenzyme
binding and
hydride transfer in Rhodobacter
capsulatus
ferredoxin/flavodoxin NADP(H) oxidoreductase
Ana Bortoloti, Inmaculada
Pérez-Dorado, Guillermina Goñi, Milagros Medina,
Juan A. Hermoso,
Néstor Carrillo and Néstor Cortez Biochimica
et Biophysica Acta - Proteins & Proteomics (2009) 1794,
199-210 (doi:10.1016/j.bbapap.2008.09.013)
Flavodoxin:
A compromise between efficiency and versatility in the electron
transfer from Photosystem I to Ferredoxin-NADP+
reductase
Guillermina Goñi, Beatriz Herguedas, Manuel
Hervás,
José R. Peregrina, Miguel A. de la Rosa, Carlos
Gómez-Moreno, José A. Navarro, Juan A. Hermoso,
Marta Martínez-Júlvez and Milagros Medina Biochimica
et Biophysica Acta - Bioenergetics (2009) 1787, 144–154 (doi:10.1016/j.bbabio.2008.12.006)
Further
Evidence for
2-Alkyl-2-carboxyazetidines as γ-Turn Inducers J.L. Baeza,
G. Gerona-Navarro, K. Thompson, M.J. Prez de Vega, L. Infantes,
M.T. García-Lopez, R. Gonzalez-Muñiz and M.
Martin-Martinez Journal
of Organic Chemistry (2009) 74, 8203-8211 (doi:10.1021/jo901712x) Molecular
Crystal Prediction Approach by Molecular Similarity: Data Mining on
Molecular Aggregation Predictors and Crystal descriptors
J. Fayos Crystal
Growth and Design (2009) 9, 3142-3153 (doi:10.1021/cg801122m)
2008
Targeted polypharmacology:
discovery of dual inhibitors of tyrosine and phosphoinositide kinases
Apsel B., Blair J.A., Gonzalez B., Nazif T.M., Feldman M.E., Aizenstein
B., Hoffman R., Williams R.L., Shokat K.M. and Knight Z.A. Nature
Chemical Biology (2008) 4, 691-699 (doi:10.1038/nchembio.117)
Crystallization
and preliminary X-ray
diffraction studies of the BTL2 lipase from extremophilic microorganism
Bacillus
thermocatenulatus
César Carrasco-López, César Godoy,
Blanca de las
Rivas, Gloria Fernández-Lorente, José M. Palomo,
José M.
Guisán, Roberto
Fernández-Lafuente, Martín
Martínez-Ripoll and Juan A.
Hermoso Acta
Crystallographica (2008) F64, 1043-1045 (doi:10.1107/S1744309108031928) A
one-pot, simple methodology for cassette randomisation and
recombination for focused directed evolution Aurelio Hidalgo, Anna
Schliessmann, Rafael Molina, Juan Hermoso and Uwe T. Bornscheuer Protein Engineering, Design &
Selection (2008) 21(9), 567-576 [PDF-copy]
Solid-Phase
Chemical Amination of a Lipase
from Bacillus thermocatenulatus To Improve Its Stabilization via
Covalent Immobilization on Highly Activated Glyoxyl-Agarose
Gloria
Fernandez-Lorente, Cesar A. Godoy, Adriano A. Mendes, Fernando
Lopez-Gallego, Valeria Grazu, Blanca de las Rivas, Jose M. Palomo, Juan
Hermoso, Roberto Fernandez-Lafuente, and Jose M. Guisan Biomacromolecules
(2008) 9, 2553–2561 [PDF-copy] Characterization
of the
p-coumaric acid decarboxylase from Lactobacillus
plantarum CECT 748(T)
Rodríguez, H., Landete, J.M., Curiel, J.A., de Las Rivas,
B., Mancheño, J.M., Muñoz, R. Journal
of Agricultural and Food Chemistry (2008) 56, 3068-3072 (doi:10.1021/jf703779s)
Las enzimas
líticas de los bacteriófagos
(Enzibióticos): Nuevas terapias contra las infecciones
bacterianas Hermoso, J.A. MADRI+D
(2008), Abril 29th [Web-access]
Los
enzibióticos progresan en un momento clave
Hermoso, J.A. Diario
Médico (2008) 16 mayo, 16-17[PDF-copy]
Structural
characterization
of a
ferredoxin-NADP(H) reductase and a flavodoxin from Rhodobacter
capsulatus by X-ray crystallography Inmaculada
Pérez-Dorado, Ana Bortolotti, Néstor Cortez and
Juan A. Hermoso in Flavins and
Flavoproteins (2008). S. Frago, C. Gómez-Moreno
and M. Medina Eds. (2008). 261-267. ISBN: 978-84-7733-017-2
The coenzyme binding site
of bacterial ferredoxin/flavodoxin-NADP(H) reductases Inmaculada
Pérez-Dorado, Juan A.
Hermoso, Guillermina Goñi, Milagros Medina, Ana Bortolotti,
Néstor Carrillo, and Néstor Cortez in Flavins and
Flavoproteins (2008). S. Frago, C. Gómez-Moreno
and M. Medina Eds. (2008). 267-273. ISBN: 978-84-7733-017-2
Crystallization and preliminary crystallographic analysis of
merohedrally twinned crystals of MJ0729, a CBS-domain protein from Methanococcus jannaschii P.
Fernández-Millán, D. Kortazar, M. Lucas, M.L.
Martínez-Chantar, E.
Astigarraga, J.A. Fernández, O. Sabas, A. Albert, J.M. Mato
and L.A.
Martínez-Cruz Acta Crystallographica (2008) F64, 605-609
Crystallization of a
flavodoxin involved in the nitrogen fixation in Rhodobacter capsulatus I. Pérez-Dorado,
A. Bortolotti, N. Cortez and J.A. Hermoso Acta Crystallographica (2008) F64, 375-377
(doi: 10.1107/S1744309108008038) 2007
The structure of the
C-terminal domain of the protein kinase AtSOS2 bound to the calcium
sensor AtSOS3 María
José
Sánchez-Barrena, Hiroaki Fujii, Ivan Angulo,
Martín
Martínez-Ripoll, Jian-Kang Zhu and Armando Albert Molecular Cell (2007) 26, 427-435 (doi:10.1016/j.molcel.2007.04.013)
Taking aim on bacterial
pathogens: from phage therapy to enzybiotics Juan A. Hermoso,
José L García, Pedro García Current Opinion in Microbiology (2007)
10(5), 461-472 (doi:10.1016/j.mib.2007.08.002)
Enhancement of the
Stability of a Prolipase from Rhizopus
oryzae Towards Aldehydes by
Saturation Mutagenesis Mirella Di Lorenzo, Aurelio
Hidalgo, Rafael Molina, Juan A. Hermoso, Guido Greco and Uwe T.
Bornscheuer Applied and Environmental Microbiology
(2007) 73(22), 7291-7299 (doi:10.1128/AEM.01176-07)
Crystallization and
preliminary X ray diffraction studies of the Choline Binding Protein F
from Streptococcus
pneumoniae Rafael Molina, Ana
González, Miriam Moscoso, Pedro García, Meike
Stelter, Richard Kahn and Juan A. Hermoso Acta Crystallographica (2007) F63, 742-745(doi:10.1107/S1744309107035865)
The complex between SOS3
and SOS2 regulatory
domain from Arabidopsis
thaliana: Cloning, expression, purification,
crystallization and preliminary X-ray analysis
Sánchez-Barrena, M.J., Moreno-Pérez, S., Angulo,
I., Martínez-Ripoll, M. and Albert, A. Acta
Crystallographica (2007) F63, 568-570(doi:10.1107/S174430910702533X)
El
Neumococo. Mecanismo patogénico J.A. Hermoso Investigación
y Ciencia (2007) 29-31 (link
to the web) Tuning of the FMN binding and
oxido-reduction properties by neighboring side chains in Anabaena
Flavodoxin S. Frago, G.
Goñi, B.
Herguedas, J.R. Peregrina, A. Serrano, I. Perez-Dorado, R. Molina, C.
Gómez-Moreno,
J.A. Hermoso,
M. Martínez-Júlvez, S. G. Mayhew and M. Medina Archives
of Biochemistry
and Biophysics (2007) 467, 206-217 (doi:10.1016/j.abb.2007.08.024)
Elucidation of the Molecular
Recognition of Bacterial Cell Wall by Modular Pneumococcal Phage
Endolysin
Cpl-1 I.
Pérez-Dorado, N. E. Campillo, B. Monterroso, D.
Hesek, M. Lee, J. A. Páez, P. García, M.
Martínez-Ripoll, J. L. García, S.
Mobashery,
M. Menéndez and J.A. Hermoso Journal
of Biological
Chemistry (2007) 282, 24990-24999 (doi:10.1074/jbc.M704317200)
Common conformational
changes in
flavodoxins induced by FMN and anion binding: The structure of Helicobacter pylori
apoflavodoxin M
Martínez-Júlvez, N
Cremades, M Bueno, I Pérez-Dorado, C Maya, S
Cuesta-López, D Prada, F Falo, J.A. Hermoso and. J Sancho Proteins: Structure, Function and
Bioinformatics (2007) 69, 581-594 (doi:10.1002/prot.21410)
Genetic Modification of the
Penicillin G Acylase Surface To Improve Its Reversible Inmobilization
on Ionic Exchangers Tamara Montes, Valeria
Grazu, Fernando
López-Gallego, Juan A. Hermoso, José L.
García,
Isabel Manso, Beatriz Galán, Ramón
González,
Roberto Fernández-Lafuente and José M.
Guisán Applied and Environmental Microbiology
(2007) 73(1), 312-319 (doi:10.1128/AEM.02107-06)
Improved stabilization of genetically
modified Penicillin G Acylase in
the presence of organic cosolvents by co-immobilization of the enzyme
with polyethylenimine Tamara Montes, Valeria
Grazu, Isabel Manso, Beatriz Galán,
Ramón González, Fernando
López-Gallego, Juan
A.
Hermoso, José L. garcía,
José M. Guisán and
Roberto Fernández-Lafuente Adv.
Synth. Catal. (2007). 349, 459-464
(doi:10.1002/adsc.200600313)
Insights into molecular plasticity of
choline binding proteins
(pneumococcal surface proteins) by SAXS Rubén M. Buey,
Begoña Monterroso, Margarita
Menéndez, Greg Diakun, Pablo Chacón, Juan Antonio
Hermoso
and J. Fernando Díaz Journal
of Molecular Biology, (2007), 365 (2), 411-424
(doi:10.1016/j.jmb.2006.09.091)
Mixed ion exchangers
supports as useful
ion exchangers for protein purification. Purification of penicillin G
acylase from E.coli Manuel Fuentes, Pilar
Batalla, Valeria
Grazu, Benevides C.C. Pessela, Cesar Mateo, Tamara Montes, Juan A.
Hermoso, José M. Guisán and Roberto
Fernández-Lafuente Biomacromolecules (2007) 8(2), 703-707
Insights into the
activation of brain
serine racemase by the multi-PDZ domain glutamate receptor interacting
protein, divalent cations and ATP Baumgart, F,
Mancheño JM, Rodríguez-Crespo, I FEBS J. (2007) 274(17), 4561-4571
Overexpression,
purification,
crystallization and preliminary structural studies of catabolic
ornithine transcarbamylase from Lactobacillus
hilgardii de las Rivas, B,
Rodríguez, H, Angulo, I, Muñoz, R,
Mancheño JM Acta Crystallographica (2007) F63, 563-567
Expression vectors for
enzyme restriction- and ligation-independent cloning for producing
recombinant His-fusion proteins de las Rivas, B, Curiel,
JM., Mancheño JM, Muñoz, R Biotechnol. Prog. (2007) 23, 680-686
Overexpression,
purification,
crystallization and preliminary structural studies of p-coumaric acid
decarboxylase from Lactobacillus
plantarum Rodríguez, H, de
las Rivas, B, Muñoz, R, Mancheño, JM Acta Crystallographica (2007) F63,
300-303
Crystal structures of Paenibacillus
polymyxa β-glucosidase B complexes reveal the
molecular
basis of
substrate specificity and give insights into the catalytic mechanism of
family I glycosidases Isorna, P., Polaina, J.,
Latorre-García. L., Cañada, F.J.,
González, B. y Sanz-Aparicio, J. Journal of Molecular Biology (2007) 371,
1204-1218
Xylanases:
Molecular
properties and applications Pastor, F.J., Gallardo, O.,
Sanz-Aparicio, J. y Díaz, P. in Industrial
Enzymes: Structure, Function and Applications (2007) Chpt.
5, 65-82, Springer NL, Polaina, Julio; MacCabe, Andrew P.
(Eds.), XII, 642 p., Hardcover. ISBN: 978-1-4020-5376-4 2006
A pharmacological map of
the PI3-K family defines a role for p110α in insulin signaling
Knight Z.A., González B., Feldman M.E., Zunder
E.R.,
Goldenberg D.D., Williams O., Loewith R., Stokoe D., Balla A., Toth B.,
Balla T., Weiss W.A., Williams R.L. and Shokat K.M.
Cell (2006) 125, 733-747 [PDF-copy] The
role of electrostatic
interactions in the antitumor activity of dimeric Rnases Notomista, E,
Mancheño JM, Crescenzi, O, Di Donato A, Gavilanes, JG,
D´Alessio, G FEBS J. (2006) 273, 3687-3697
A complementary microscopy
analysis of
Sticholysin II crystals on lipid films: Atomic Force and Transmission
Electron characterizations Mancheño JM,
Martín-Benito J, Gavilanes JG, Vázquez L Biophys. Chem. (2006) 119(3), 219-223
Chemical
modification of protein surfaces to improve their reversible enzyme
immobilization on ionic exchangers Tamara Montes, Valeria
Grazu, Fernando López-Gallego, Juan
A.
Hermoso, José M. Guisán and Roberto
Fernández-Lafuente Biomacromolecules
(2006). 7, 3052-3058 (doi:10.1021/bm060527p)
Cell signaling and function
organized by PB1 domain interactions Moscat, J., Diaz-Meco,
M.T., Albert, A. and Campuzano, S. Molecular Cell (2006) 23, 631-640
[PDF-copy]
X-ray and Neutron Diffraction Approaches
to the Structural Analysis of
Protein-Lipids Interactions Juan A. Hermoso,
José M. Mancheño and Eva Pebay-Peyroula in Protein-Lipid
Interactions. New Approaches and Emerging Concepts. Springer Verlag
Berlin Heidelberg, (2006). pp.63-100 (doi:10.1007/3-540-28435-4_3)
La Quimica de
la vida a escala atomica Hermoso,
J.A., Sanz-Aparicio, J.A. and Albert, A. Anales
de Química (2006) 102(4), 15-22[PDF-copy] 2005
Insights into pneumococcal
pathogenesis from crystal structure of the modular teichoic acid
phosphorylcholine esterase Pce Hermoso, J., Lagartera, L.,
Gonzalez, A., Stelter, M., Garcia, P., Martinez-Ripoll, M., Garcia,
J.L. and Menendez, M. Nature
Structural and Molecular Biology (2005) 12, 533-538(doi:10.1038/nsmb940)
C-terminal
Tyrosine of Ferredoxin-NADP+ reductase in the hydride transfer
processes with NAD(P)+/H J. Tejero, I.
Pérez-Dorado, C. Maya, M.
Martínez-Júlvez, J. Sanz-Aparicio, C.
Gómez-Moreno, J.A. Hermoso and M. Medina Biochemistry
(2005) 44, 13477-13490 (doi:10.1021/bi051278c)
Structure of the Functional Domain of phi
29 Replication Organizer:
Insights into oligomerization and DNA binding J.L. Asensio, A. Albert, D.
Muñoz-Espín, C.
Gonzalez, J.
Hermoso, L. Villar, J. Jiménez-Barbero, M.
Salas, and W.J.J.
Meijer Journal
of Biological Chemistry (2005) 280, 20730-20739 (doi:10.1074/jbc.M501687200)
Hydralysins: A new category
of
beta-pore-forming toxins in cnidaria. Characterization and preliminary
structure-function analysis Sher DJ, Fishman Y, Zhang
M, Lebendiker M, Gaathon A, Mancheño JM, Zlotkin E J. Biol. Chem. (2005) 280(24), 22847-22855
BzdR, a repressor that
controls the
anaerobic catabolism of benzoate in Azoarcus sp. CIB,
is the first
member of a new subfamily of transcriptional regulators Barragán MJ,
Blázquez B, Zamarro MT, Mancheño JM,
García JL, Díaz E, Carmona M J. Biol. Chem. (2005) 280(11), 10683-10694
The 1.49 A resolution crystal structure
of PsbQ from photosystem II of Spinacia
oleracea
reveals a PPII structure in the N-terminal region Balsera. M., Arellano,
J.B., Revuelta, J.L., de las Rivas, J. and
Hermoso, J. Journal
of Molecular Biology (2005) 350, 1051-1060
(doi:10.1016/j.jmb.2005.05.044)
Crystallization and
preliminary X-ray diffraction studies of the pneumococcal teichoic acid
phosphorylcholine esterase Pce Lagartera, L., Gonzalez,
A., Stelter, M., Garcia, P., Kahn, R., Menendez, M. and Hermoso, J. Acta Crystallographica (2005) F61,
221-224 (doi:10.1107/S1744309105001636)
Conformational
engineering of lipases via directed immobilization. Improving the
resolution of chiral drugs J.M Palomo, G.
Fernández-Lorente,
C. Ortiz, R. L. Segura, C. Mateo, M. Fuentes, J. Hermoso, R.
Fernández- Lafuente and J. M Guisán Medicinal
Chemistry
reviews-online (2005) 2(5), 369-378
(doi:10.2174/156720305774330494)
Structural Analysis of the Laetiporus
sulphureous Hemolytic Pore-forming Lectin in Complex with
Sugars Mancheño, J.M.,
Tateno, H., Goldstein, I.J., Martínez-Ripoll, M. and
Hermoso, J.A. Journal of Biological Chemistry (2005)
280, 17251-17259
Activation in the family of
Candida rugosa
isolipases by polyethylene glycol Otero, C.,
Fernández-Pérez, M., Hermoso, J.A. and
Martínez-Ripoll, M. Journal of Molecular Catalysis B:
Enzymatic (2005) 32, 225-229
Structural Analysis of
Interactions for Complex Formation between Ferredoxin-NADP+ Reductase
and Its Protein Partners Mayoral, T.,
Martinez-Julvez, M., Perez-Dorado, I., Sanz-Aparicio, J., Gomez-Moreno,
C., Medina, M. and Hermoso, J.A. Proteins: Structure, Function, and
Bioinformatics (2005) 59, 592-602
Pneumococcal
phosphorylcholine esterase, Pce, contains a zinc binuclear
center that is essential for substrate binding and catalysis L. Lagartera, A.
González, J. A. Hermoso, J. L.
Saíz, P. García, J. L. García and M.
Menéndez Protein
Science (2005) 14, 3013-3024 (doi:10.1110/ps.051575005)
The Ferredoxin-NADP(H) Reductase from Rhodobacter capsulatus:
Molecular
Structure and Catalytic Mechanism I. Nogués, I.
Pérez-Dorado, S. Frago, C. Bittel,
S. G.
Mayhew, C. Gómez-Moreno, J.
A. Hermoso, M. Medina, N. Cortez,
and N. Carrillo Biochemistry
(2005) 44, 11730-11740 (doi:10.1021/bi0508183) Probing the Determinants of Substrate
Specificity of a Feruloyl
Esterase, AnFaeA, from Aspergillus
niger C.B. Faulds, R. Molina, R.
Gonzalez, F. Husband, N. Juge, J.
Sanz-Aparicio and J. A.
Hermoso FEBS
Journal (2005) 272, 4362-4371
(doi:10.1111/j.1742-4658.2005.04849.x)
The structure of the
functional dimeric domain of phage f29 protein p16.7 reveals mechanisms
for oligomerization and DNA binding Asensio, JL, Albert, A,
Muñoz-Espín, D, González, C, Hermoso,
JA, Villar,
L, Jiménez-Barbero, J, Salas, M and Meijer, W Journal of Biological Chemistry (2005)
280, 20730-20739
Structural
basis for membrane
anchorage of viral phi 29 DNA during replication Albert,
A., Munoz-Espin, D., Jimenez, M., Asensio, J.L.,
Hermoso, J.A., Salas, M., Meijer, W.J. Journal
of Biological
Chemistry (2005)
280, 42486-42489 (doi:10.1074/jbc.C500429200)
The structure of the Arabidopsis
thaliana SOS3: molecular mechanism of sensing calcium for
salt stress response Sánchez-Barrena,
M.J., Martínez-Ripoll, M., Zhu, J.K. and Albert, A. Journal of Molecular Biology (2005) 345,
1253-1264 (doi:10.1016/j.jmb.2004.11.025)
Rat liver betaine
homocysteine
S-methiltransferase equilibrium unfolding: Insights into intermediate
structure through tryptophan substitutions Garrido, F., Gasset, M.,
Sanz-Aparicio, J., Alfonso, C. and Pajares, M.A. Biochemical Journal (2005) 391, 589-599 2004
ESCRT-II, an
endosome-associated complex
required for protein sorting: crystal structure and interactions with
ESCRT-III and membranes
Teo H., Perisic O., González B. and Williams R.L.
Developmental Cell (2004) 7, 559-569 (doi:10.1016/j.devcel.2004.09.003)
The structure of human inositol
1,4,5-trisphosphate
3-kinase: The substrate binding reveals why it is not a
phosphoinositide 3-kinase
González B., Schell M.J., Letcher, A.J., Veprintsev, D.B.,
Irvine R. and Williams R.L.
Molecular Cell (2004) 15,689-701 (doi:10.1016/j.molcel.2004.08.004) SOS3
(salt overlay
sensitive 3) from Arabidopsis
thaliana: expression, purification, crystallization and
preliminary X-ray analysis Sánchez-Barrena,
M.J., Martínez-Ripoll, M., Zhu, J.K. and Albert, A. Acta Crystallographica (2004) D60,
1272-1274 (doi:10.1107/S0907444904008728)
Structure of concanavalin A
at pH 8: bound solvent and crystal contacts Lopez-Jaramillo FJ,
Gonzalez-Ramirez LA, Albert A, Santoyo-Gonzalez F, Vargas-Berenguel A,
Otalora F Acta Crystallographica (2004) D60,
1048-1046 (doi:10.1107/S0907444904007000)
Peptide AS-48: prototype of
a new class of cyclic bacteriocins Maqueda M, Galvez A, Bueno
MM, Sanchez-Barrena MJ, Gonzalez C, Albert A, Rico M, Valdivia E. Current Protein and Peptide Science
(2004) 5, 399-416 (doi:10.2174/1389203043379567)
Crystallization and
preliminary crystallographic analysis of a novel haemolytic lectin from
the mushroom Laetiporus
sulphureus Mancheño, J.M.,
Hiroaki, T., Goldstein, I.J. and Hermoso, J.A. Acta Crystallographica (2004) D60,
1139-1141 (doi:10.1107/S0907444904007991)
Crystallization of a
proteolyzed form of
the horse pancreatic lipase-related protein 2: structural basis for the
specific detergent requirement Mancheño, J.M.,
Jayne, S., Kerfelec, B., Chapus, C., Crenon, I. and Hermoso, J.A. Acta Crystallographica (2004) D60,
2107-2109 (doi:10.1107/S0907444904024229)
Crystallization and
preliminary X-ray diffraction analysis of ferredoxin-NADP(H) reductase
from Rhodobacter
capsulatus Perez-Dorado. I., Bittel,
C., Cortez, N. and Hermoso, J.A. Acta Crystallographica (2004) D60,
2332-2335 (doi:10.1107/S090744490402640X)
Stabilization of Penicillin
G Acylase
from Escherichia coli:
Site-Directed Mutagenesis of the Protein Surface
To Increase Multipoint Covalent Attachment Abian, O., Grazu, V.,
Hermoso, J.A., Gonzalez, R., Garcia, J.L., Fernandez-Lafuente, R. and
Guisan, J.M. Applied and Environmental Microbiology
(2004) 70, 1249-1251 (doi:10.1128/AEM.70.2.1249-1251.2004)
Phenotypic selection and
characterization of randomly produced non-haemolytic mutants of the
toxic sea anemone protein Sticholysin II Alegre-Cebollada,
J,
Lacadena, V, Oñaderra, M, Mancheño, JM, Gavilanes
JG, Martínez del Pozo, A FEBS Lett. (2004) 575, 14-18
(doi:10.1016/j.febslet.2004.08.031)
The Crystal Structure of
Feruloyl
Esterase A from Aspergillus
niger Suggests Evolutive Functional
Convergence in Feruloyl Esterase Family Hermoso, J.A.,
Sanz-Aparicio, J., Molina, R., Juge, N., Gonzalez, R. and Faulds, C.B. Journal of Molecular Biology (2004) 338,
495-506 (doi:10.1016/j.jmb.2004.03.003)
Functional characterization
of the yeast Ppz1 phosphatase inhibitory subunit Hal3: a mutagenesis
study Muñoz, I, Ruiz,
A, Marquina, M, Barcelo, A, Albert, A, Ariño, J. Journal of Biological Chemistry (2004)
279, 42619-42627 (doi:10.1074/jbc.M405656200) 2003
Estructura de
proteínas por Cristalografía de rayos X Hermoso, J.A. y
Martínez-Ripoll, M. in Estructura de
proteínas (2003). Gómez-Moreno, C.
and Sancho Sanz, J., coord., Ariel Ciencia, ISBN: 84-344-8061-6,
pp.
253-285
Crystallization
and preliminary X-ray diffraction studies of two
different crystal forms of the lipase 2 isoform from the yeast Candida
rugosa José
M. Mancheño, María A. Pernas,
María L.
Rúa and J.A.
Hermoso Acta
Crystallographica (2003) D59, 499-501
(doi:10.1107/s0907444902022539)
Structural aspects on rat
liver S-adenosylmethionine synthesis Pajares, M.A., Gasset, M.,
Sanz-Aparicio, J., Calvete, J.J. and Rodriguez Arrondo, J.L. Advances in Biological Chemistry (2003)
2, 31-41
Active-site-mutagenesis
study of rat liver betaine-homocysteine S-methyltransferase González, B.,
Campillo, N., Garrido, F., Gasset, M., Sanz-Aparicio, J. and Pajares,
M.A. Biochem. J. (2003) 370, 945-952
Involvement
of the Pyrophosphate and 2'-Phosphate Binding Regions of
Ferredoxin-NADP+ Reductase in Coenzyme Specificity Jesús
Tejero, Marta
Martínez-Júlvez,
Tomás Mayoral, Alejandra Luquita, J. Sanz-Aparicio, Juan A.
Hermoso, John K. Hurley, Gordon Tollin, Carlos
Gómez-Moreno
and
Milagros Medina Journal
of Biological Chemistry (2003), 278(49), 49203-49214
(doi:10.1074/jbc.M307934200)
Structure of Bacteriocin
AS-48: From Soluble State to Membrane Bound State Sánchez-Barrena,
M.J., Martinez-Ripoll, M., Gálvez, A., Valdivia, E.,
Maqueda, M., Cruz, V. and Albert, A. Journal of Molecular Biology (2003) 334,
541-549 (doi:10.1016/j.jmb.2003.09.060)
Structural Insights into
the
Lipase/esterase Behavior in the Candida
rugosa Lipases Family: Crystal
Structure of the Lipase 2 Isoenzyme at 1.97 A Resolution Mancheño, J.M.,
Pernas, M.A., Martínez, M.J., Ochoa, B., Rúa,
M.L. and Hermoso, J.A. Journal of Molecular Biology (2003) 332,
1059-1069
Crystal
Structures of Methionine Adenosyltransferase complexed with
Substrates and Products Reveal the Methionine-ATP Recognition and give
Insights into the Catalytic Mechanism B.
González, M. Pajares, Juan
A. Hermoso, D. Guillerm, G.
Guillerm & J. Sanz-Aparicio Journal
of Molecular Biology (2003) 331, 407-416
(doi:10.1016/s0022-2836(03)00728-9)
Structural Basis for
Selective Recognition of Pneumococcal Cell Wall by Modular Endolysin
from Phage Cp-1 Hermoso, J.A., Monterroso,
B., Albert,
A., Galán, B., Ahrazem, O., García, P.,
Martínez-Ripoll, M., García, J.L., and
Menéndez,
M. Structure (2003) 11, 1239-1249
Crystal and Electron
Microscopy
Structures of Sticholysin II Actinoporin Reveal Insights into the
Mechanism of Membrane Pore Formation. Mancheño, J.M.,
Martín-Benito, J., Martínez-Ripoll, M.,
Gavilanes, J.G. and Hermoso, J.A. Structure (2003) 11, 1319-1328
2002
Crystallization and
preliminary X-ray study of recombinant betaine-homocysteine
S-methyltransferase from rat liver González, B.,
Pajares, M.A., Too, H.-P., Garrido, F., Blundell, T.L. and
Sanz-Aparicio, J. Acta Crystallographica (2002) D58,
1507-1510
Crystallization and
preliminary X-ray
diffraction studies of the complete modular endolysin from Cp-1, a
phage infecting Streptococcus
pneumoniae Monterroso, B., Albert, A.,
Martínez-Ripoll, M., García, P.,
García, J.L.,
Menéndez, M. and Hermoso, J.A. Acta Crystallographica (2002) D58,
1487-1489
Crystallization and
preliminary X-ray
diffraction studies of the water-soluble state of the pore-forming
toxin sticholysin II from the sea anemone Stichodactyla helianthus Mancheño, J.M.,
Martínez-Ripoll, M., Gavilanes, J.G. and Hermoso, J.A. Acta Crystallographica (2002) D58,
1229-1231
Activation
of Horse PLRP2 by Bile Salts does not require Colipase Sandrine
Jayne, B. Kerfelec, E. Foglizzo, S. Granon, Juan A. Hermoso,
C. Chapus and I. Crenon Biochemistry
(2002. 41, 8422-8428 (doi:10.1021/bi025867j)
Structural
basis for the kinetics of Candida
rugosa Lip1 and Lip3
isoenzymes Pernas,
M., López, C., Prada, A., Hermoso, J. and
Rúa,
M.L. Colloids
and Surfaces B: Biointerfaces. (2002) 26, 67-74
(doi:10.1016/S0927-7765(01)00309-5)
Probing the
Role of the Glutamic 139 Residue in Anabaena
Ferredoxin-NADP+ Reductase in its interaction with substrates Merche
Faro, Susana
Frago, Tomás Mayoral, Juan
A. Hermoso,
J. Sanz-Aparicio, Carlos Gómez-Moreno, and Milagros Medina European
Journal of Biochemistry (2002) 269, 4938-4947
(doi:10.1046/j.1432-1033.2002.03194.x) TrwD, the Hexameric Traffic
ATPase Encoded by Plasmid R388, Induces Membrane Destabilization and
Hemifusion of Lipid Vesicles Machón, C.,
Rivas, S., Albert, A., Goñi, F.M. and de la Cruz, F. Journal of Bacteriology (2002) 184,
1661-1668
Mechanism
of Coenzyme Recognition and Binding Revealed by Crystal
Structure Determination of Ferredoxin-NADP+ Reductase Complexed with
NADP+ Juan A. Hermoso,
Tomás Mayoral, Merche Faro, Carlos
Gómez-Moreno, J. Sanz-Aparicio and Milagros Medina Journal of
Molecular Biology (2002) 319, 1133-1142
(doi:10.1016/S0022-2836(02)00388-1)
Estructural Enzymology of
Li+-sensitive/Mg2+-dependent Phosphatases Patel, S., Martinez-Ripoll,
M., Blundell, T.L. and Albert, A. Journal of Molecular Biology (2002) 320,
1087-1094 2001
Probing the
Determinants of Coenzyme Specificity in Ferredoxin-NADP+
Reductase by Site-directed Mutagenesis Medina
M, Luquita A, Tejero J, Hermoso
J, Mayoral T, Sanz-Aparicio J,
Grever K, Gomez-Moreno C Journal
of Biological Chemistry (2001) 276(15), 11902-11912
(doi:10.1074/jbc.M009287200)
Role of a
Cluster of Hydrophobic Residues Near the FAD Cofactor in Anabaena PCC 7119
Ferredoxin-NADP+ Reductase for Optimal Complex
Formation and Electron Transfer to Ferredoxin Marta
Martínez-Júlvez, Isabel
Nogués, Merche
Faro, John K. Hurley, Tammy B. Brodie,
Tomás Mayoral, J. Sanz-Aparicio, Juan A. Hermoso,
Marian
Stankovich
Milagros Medina, Gordon Tollin and Carlos Gómez-Moreno Journal
of Biological Chemistry (2001) 276(29), 27498-27510
(doi:10.1074/jbc.M102112200)
Regioselective
Resolution of 1,n-Diols Catalysed by Lipases: A Rational
Explanation oF the Enzymatic Selectivity I.
Borreguero, J.M. Sanchez-Montero, J.V. Sinisterra, A. Rumbero, J.A.
Hermoso and A.R. Alcantara Journal
of Molecular Catalysis B: Enzymatic (2001) 11, 1013-1024
(doi:10.1016/S1381-1177(00)00086-2)
Influence of the
conformational flexibility on the kinetics and dimerisation process of
two Candida rugosa lipase isoenzymes Pernas, M.A.,
López, C., Rúa, M.L. and Hermoso, J. FEBS Letters (2001) 501, 87-91 2000
The X-ray Structure of the
FMN-binding
Protein AtHal3 Provides the Structural Basis for the Activity of a
Regulatory Subunit Involved in Signal Transduction Albert, A.,
Martinez-Ripoll, M., Espinosa-Ruiz, A., Yenush, L.,
Culiañez-Macia, F.A. and Serrano, R. Structure (2000) 8, 961-969
Critical
Role of Micelles in Pancreatic Lipase Activation Revealed by
Small Angle Neutron Scattering D.
Pignol, L. Ayvazian, B. Kerfelec, P. Timmins, I. Crenon, J.A.
Hermoso, J.C. Fontecilla-Camps and C. Chapus Journal
of Biological Chemistry (2000) 6, 4220-4224
(doi:10.1074/jbc.275.6.4220)
Direct
Evolution of β-glucosidase A from Paenibacillus
polymyxa to
thermal Resistance G.
González-Blasco, J. Sanz-Aparicio, J. Hermoso and
J.
Polaina Journal
of Biological Chemistry (2000) 275(18), 13708-13712
(doi:10.1074/jbc.275.18.13708)
X-ray structure of yeast
Hal2p, a major target of lithium and sodium toxicity, and
identification of framework interactions determining cation sensitivity
Albert, A., Yenush, L.,
Gil-Mascarell, M.R., Rodriguez, P.L., Patel, S., Martinez-Ripoll, M.,
Blundell, T.L. and Serrano, R. Journal of Molecular Biology (2000) 295,
927-938
The Crystal
Structure of Tetrameric Methionine Adenosyltransferase from
rat liver Reveals the Methionine Binding-site B.
González, M. Pajares, Juan
A. Hermoso, L. Alvarez, F.
Garrido and J. Sanz-Aparicio Journal
of Molecular Biology (2000) 300, 363-375
(doi:10.1006/jmbi.2000.3858)
Structural
Basis of the catalytic Role of Glu301 in Anabaena PCC7119
Ferredoxin-NADP+ Reductase Revealed by X-Ray Crystallography T.
Mayoral, M. Medina, J. Sanz-Aparicio, C. Gomez-Moreno and J.
Hermoso Proteins:
Structure, Function and Genetics (2000) 38, 60-69
(doi:10.1002/(SICI)1097-0134(20000101)38:1<60::AID-PROT7>3.0.CO;2-B)
Biochemical and Physical
Characterization of Parvovirus Minute Virus of Mice Virus-like
Particles Hernando, E., Llamas-Saiz,
A.L., Foces-Foces, C., McKenna, R., Portman, I., Agbandje-McKenna, M.
and Almendral, J.M. Virology (2000) 267, 299-309 1999
Making the most of
commercial sparse-matrix protein crystallization screening kits Albert, A. and
Martinez-Ripoll, M. Journal of Applied Crystallography (1999)
32, 336-338
Molecular study of the rat
liver NADH: Cytochrome c oxidoreductase complex during development and
ageing Torres-Mendoza, C., Albert,
A. and Cruz Arriaga, M. Molecular and Cellular Biochemistry
(1999) 195, 133-142
A six-stranded double-psi
beta barrel is shared by several protein superfamilies Castillo, R.M., Mizuguchi,
K., Dhanaraj, V., Albert, A., Blundell, T.L. and Murzin, A.G. Structure (1999) 7, 227-236
Acyclic
Phenylalkanediols as Substrates for the study of Enzyme
recognition: Part II: Regioselective Acylation by by Porcine Pancreatic
Lipase: A structural Hypothesis of the Enzymatic Selectivity I.
Borreguero, J.V. Sinisterra, A. Rumbero, J.A. Hermoso, M.
Martínez-Ripoll and A.R. Alcantara Tetrahedron
(1999) 55, 14961-14974 (doi:10.1016/S0040-4020(99)00942-4) Synthesis,
structure and redox properties of
ruthenium complexes containing the tpm facial and the trpy meridional
tridentate ligands: Crystal structures of [RuCl3(trpy)]
and [Ru(tpm)(py)3](PF6)2 Laurent, F.,
Plantalech, E.,
Donnadieu, B., Jiménez, A., Hernández, F.,
Martínez-Ripoll, M., Biner, M. and Llobet, A.
Polyhedron (1999) 18, 3321-3331 1998
Crystal structure of
aspartate
decarboxylase at 2.2 Å resolution provides evidence for an
ester in
protein self-processing
Albert, A., Dhanaraj, V., Genschel, U., Khan, G., Ramjee, M.K., Pulido,
R., Sibanda, B.L., von Delft, F., Witty, M., Blundell, T.L., Smith,
A.G. and Abell, C. Nature
Structural
and Molecular Biology (1998) 5, 289-293 (doi:10.1038/nsb0498-289)
Pancreatic
Lipase related proteins type I: a double mutation restores a
significant lipase activity I.
Crenon , S. Jayne, B. Kerfelec, J.
Hermoso, D. Pignol and C. Chapus Biochemical
and Biophysical Research Communications (1998) 246,
513-517 (doi:10.1006/bbrc.1998.865
Role of the
R100 and R264 from Anabaena
PCC7119 Ferredoxin-NADP+
Reductase for Optimal NADP+ binding and electron transfer Marta
Martínez-Julvez, Juan
A. Hermoso, John Hurley,
Tom s
Mayoral, J. Sanz-Aparicio, Gordon Tollin, Carlos
Gómez-Moreno
and Milagros Medina Biochemistry
(1998) 37(51), 17680-17691
(doi:10.1021/bi981718i)
The lipase/colipase complex is activated
by a micelle: neutron crystallographic evidence D.
Pignol, J. Hermoso
, B. Kerfelec, I. Crenon, C. Chapus and J.C.
Fontecilla-Camps Chemistry
and Physics of Lipids (1998) 93, 123-129
(doi:10.1016/s0009-3084(98)00036-x) Ion
pairing between lipase and colipase plays a critical
role in catalysis L.
Ayvazian, I. Crenon, J.
Hermoso, D. Pignol, C. Chapus and B.
Kerfelec Journal
of Biological Chemistry (1998) 273(50),
33604-33609 (doi:10.1074/jbc.273.50.33604)
Crystal Structure of
β-Glucosidase A
from Bacillus polymyxa:
Insights into the Catalytic Activity in Family
1 Glycosyl Hydrolases Sanz-Aparicio, J., Hermoso,
J.A., Martinez-Ripoll, M., Lequerica, J.L. and Polaina, J. Journal of Molecular Biology (1998) 275,
491-502 (doi:10.1006/jmbi.1997.1467) Pancreatic
Lipase related proteins type I: specialized lipase or an
inactive enzyme I.
Crenon , E. Flogizzo, B. Kerfelec, A. Virine, D. Pignol, J. Hermoso,
J. Bonicel and C. Chapus Protein
Engineering (1998) 112, 135-142
(doi:10.1093/protein/11.2.135)
Structural
basis of increased resistence to thermal denaturation
induced by single aminoacid substitution in the sequence of
β-glucosidase A from Bacillus
polymyxa J.
Sanz-Aparicio, J. Hermoso,
M. Martínez-Ripoll, B.
González, C. López-Camacho and J. Polaina Proteins:
Structure, Function and Genetics (1998) 33, 567-576
(doi:10.1002/(SICI)1097-0134(19981201)33:4<567::AID-PROT9>3.0.CO;2-U)
Functional implications of
the structure of the murine parvovirus, minute virus of mice Agbandje-McKenna, M.,
Llamas-Saiz, A.L., Wang, F., Tattersall, P. and Rossmann, M.G. Structure (1998) 6, 1369-1381
Amide
complexes of zirconium, rhodium, and iridium: Synthesis and reactivity.
X-ray crystal structures of (η5-C5H5)2Zr(NHC6H4-o-SMe)2
and [Rh(μ-SC6H4-o-NHMe)(COD)]2 Fandos, R., Martínez-Ripoll,
M., Otero, A., Ruiz, M.J., Rodríguez, A. and Terreros, P. Organometallics
(1998) 17, 1465-1470
Enantiomerically
Pure α-Alkylidene β-Amino Esters from
Assymetric Addition of Metal
Dienolates to N-Sulfinylimines Garcia-Ruano,
J.L., Fernandez, I., del Prado Catalina, M., Hermoso,
J.A., Sanz-Aparicio, J. and
Martinez-Ripoll, M. Journal
of Organic Chemistry
(1998)
63, 7157-7161 1997
Structure Determination of
Minute Virus of Mice Llamas-Saiz, A.L.,
Agbandje-McKenna, M., Wikoff, W.R., Bratton, J., Tattersall, P. and
Rossmann, G. Acta Crystallographica (1997) D53, 93-102
Purification,
Crystallization and
Preliminary X-ray Diffraction Studies of C-Phycocyanin and
Allophycocyanin from Spirulina
platensis Moreno, A., Bermejo, R.,
Talavera, E., Alvarez-Pez, J.M., Sanz-Aparicio, J. and Romero, A. Acta
Crystallographica (1997) D53, 321-326
Aspartic proteinases: From
the first X-ray photos of pepsin crystals to hundreds of 3-D structures
Blundell, T.L., Guruprasad,
K., Albert, A., Williams, M., Sibanda, B.L. and Dhanaraj, V. Current Science (1997) 72, 483-489
Neutron
Crystallographic Evidence of Lipase/Colipase Complex activation
by a Micelle J. Hermoso, D.
Pignol, S. Penel, M. Roth, C. Chapus and J.C.
Fontecilla-Camps EMBO
Journal (1997) 16(18), 5531-5536
(doi:10.1093/emboj/16.18.5531)
The
crystal
structure of Canavalia
brasiliensis lectin suggest a
correlation between its quaternary conformation and its distinct
biological properties from concanavalin A J.
Sanz-Aparicio, J. Hermoso,
B. Grangeiro, J.J. Calvete and S.C.
Cavada.
T. FEBS
Letters (1997) 405, 114-118
(doi:10.1016/s0014-5793(97)00137-3)
Packing
Modes in Nitrobenzene Derivatives. I. The Single Stacks Andre,
I., Foces-Foces, C., Cano, F.H. and Martinez-Ripoll, M. Acta
Crystallographica
(1997)
B53, 984-995
Packing
Modes in Nitrobenzene Derivatives. II. The 'Pseudo-Herringbone'
Mode Andre,
I., Foces-Foces, C., Cano, F.H. and Martinez-Ripoll, M. Acta
Crystallographica
(1997)
B53, 996-1005
A New
Polymorph of Li4P2O7 Daidouh,
A., Veiga, M.L., Pico, C. and Martinez-Ripoll, M. Acta
Crystallographica
(1997)
C53, 167-169
Strain
Effects in Protonated Carbonyl Compounds. An Experimental and ab
Initio
Treatment of Acyclic Carboxamides and Ketones Homan,
H., Herreros, M., Notario, R., Abboud, J.L., Essefar, M., Mo,
O., Yanez,
M., Foces-Foces, C., Ramos-Gallardo, A., Martinez-Ripoll, M., Vegas,
A., Molina,
M.T., Casanovas, J., Jimenez, P., Roux, M.V. and Turrion, C. Journal
of Organic Chemistry
(1997)
62, 8503-8512
Sulfur-Directed
Asymmetric 1,3-Dipolar Cycloadditions of Azomethine
Ylides with
Enantiopure Sulfinimines Viso,
A., Fernandez, R., Guerrero-Strachan, C., Alonso, M.,
Martinez-Ripoll, M.
and Andre, I. Journal
of Organic Chemistry
(1997)
62, 2316-2317
Polymorphism
in an oligo(aryl ether ketone) Rueda,
D.R., Zolotukhin, M.G., Andre, I., Martinez-Ripoll, M., Abajo,
J. and
Alvarez, J.C. Macromolecular
Chemistry and Physics (1997) 198, 2089–2099
(doi:10.1002/macp.1997.021980707)
Organoammonium
diphosphopentamolybdates (VI): influence of organic
cations and
anion protonation on crystal packing and geometrical features of
polyanion Aranzabe,
A., Wery, A., Martin, S., Gutierrez-Zorrilla, J.M., Luque,
A.,
Martinez-Ripoll, M. and Roman, P. Inorganica
Chimica Acta
(1997)
255, 35-45
Rhodium(I)
fluorothiolate complexes as hydroformylation catalyst
precursors.
Crystal structure of two polymorphs of trans-[Rh(SC6F5)(CO)(PPh3)2] Garcia-Fierro,
J.L., Martinez-Ripoll, M., Merchan, M.D., Rodriguez, A.,
Terreros, P., Torrens, P. and Vivar-Cerrato, M.A. Journal
of Organometallic Chemistry
(1997)
544, 243-255
Configurational
and Conformational Study of New Esters Derived from
2-Methyl-2-
azabicyclo[2.2.2] octan-5-syn(anti)-ols by NMR
spectroscopy and X-ray
Crystallography Fernandez,
M.J., Huertas, R., Toledano, M.S., Galvez, E.,
Server-Carrio, J. and
Martinez-Ripoll, M. Magnetic
Resonance in Chemistry
(1997) 35, 821-828 10H+-2,3-Benzo-1,4-dioxa-7,10,13-triazacyclopentadec-2-ene-6,14-dione
Picrate Hydrate (1/1/1)
G. Hundal, S. Kumar, M.S. Hundal, H. Singh, J. Sanz-Aparicio and M.
Martinez-Ripoll Acta
Crystallographica (1997) C53, 799-801 (doi:10.1107/S0108270196015041) 1996
Lipase
Activation by Nonionic Detergents: The crystal structure of
porcine lipase-colipase-tetraethylene glycol monooctyl ether complex J. Hermoso, D.
Pignol, B. Kerfelec, I. Crenon, C. Chapus and J.
Fontecilla-Camps Journal
of Biological Chemistry (1996) 271(30), 18007-18016
(doi:10.1074/jbc.271.30.18007)
Protein engineering loops
in aspartic
proteinases: site-directed mutagenesis, biochemical characterization
and X-ray analysis of chymosin with a replaced loop from rhizopuspepsin
Nugent, P.G., Albert, A.,
Orprayoon, P., Pitts, J.E., Blundell, T.L. and Dhanaraj, V. Protein Engineering (1996) 9, 885-893
Structural Analysis of a
Mutation in Canine Parvovirus Which Controls Antigenicity and Host
Range Llamas-Saiz, A.L.,
Agbandje-Mckenna, M., Parker, J.S.L., Wahid, A.T.M., Parrish, C.R. and
Rossmann, M.G. Virology (1996) 225, 65-71 An
18-chloro-4α-hydroxy derivative of 19-acetylteupolin
IV: A neo-clerodane diterpenoid of biological interest
Hundal, G. and Martinez-Ripoll, M. Acta
Crystallographica (1996) C52, 3157-3159
Structure-Based
Rationalization of the Ionophore
Character of Lead-Selective Amide-Ether-Amine-Containing Macrocycles:
2,3-Benzo-1,4-dioxa-7,11,15-triazacyclo heptadec-2-ene-6,16-dione and
2,3-Benzo-1,4-dioxa-7,10,13 triazacyclopentadec-2-ene 6,14-dione Hundal,
G., Martínez-Ripoll, M., Sanz-Aparicio, J.,
Hundal, M.S., Singh, H., Kumar, S. and Singh, R. Acta
Crystallographica (1996) C52,
1232-1236
Bis(triethanolamine)calcium
3,5-Dinitro-benzoate Hundal,
G.,
Martínez-Ripoll, M., Hundal, M.S. and Poonia, N.S. Acta
Crystallographica (1996) C52,
789-792
Dimeric
Aqua(3,5-dinitrobenzoato)(triethyl-eneglycol)calcium(II) 3,5
Dinitrobenzoate
Hydrate Hundal,
G.,
Martínez-Ripoll, M., Hundal, M.S. and Poonia, N.S. Acta
Crystallographica (1996) C52, 786-789
Apiananes:
C23
Terpenoids with a New Type of Skeleton from Salvia apiana Luis,
J.G.,
Lahlou, E.H., Andres, L.S., Hundal, G. and Martínez-Ripoll,
M. Tetrahedron
Letters (1996) 37,
4213-4216
New
Models for the Study of the Racemization Mechanism
of Carbodiimides. Synthesis and Structure (X-ray Crystallography and 1H
NMR)
of Cyclic Carbodiimides Molina,
P., Alajarin, M., Sanchez-Andrada, P.,
Server-Carrió, J., Martínez-Ripoll, M., Anderson,
J.E., Jimeno, M.L. and Elguero, J. Journal of
Organic Chemistry (1996)
61, 4289-4299
ansa-Niobocene
Complexes: Synthesis and
Characterization of Novel Complexes [Me2Si(η5-C5H4)2]
NbCl2 and
[Me2Si(η5-C5H4)2]NbCl(RC-CR)
(R=Me,Ph). Xray
Crystal Structure of [Me2Si(η5-C5H4)2]
NbCl(MeC-CMe) Antiñolo,
A.,
Martínez-Ripoll, M., Mugnier, Y., Otero, A., Prashar, S. and
Rodriguez, A.M. Organometallics
(1996) 15, 3241-3243 (doi:10.1021/om960275g)
Synthetic
Ionophores. 13. Pyridine-Diamide-Diester
Receptors: Remarkable Effect of Amide Substi-tuents on Molecular
Organization
and Ag+ Selectivity Kumar,
S., Hundal, M., Kaur, N., Singh, R., Singh, H.,
Hundal, G., Martínez-Ripoll, M. and Sanz-Aparicio, J. Journal
of Organic Chemistry (1996) 61, 7819-7825
(doi:10.1021/jo960859s)
Steroselective
Nucleophilic Epoxidation of Hydroxy
Vinyl Sulfoxide Derivatives Fernández
de la
Pradilla, R., Manzano, P., Priego, J., Viso, A.,
Martínez-Ripoll, M. and
Rodríguez, A. Tetrahedron
Letters
(1996) 37, 6793-6796 (doi:10.1016/S0040-4039(96)01481-5)
Diastereoselective
Formation of an [μ4-(1Z)-Sulfinyl
diene]iron(0) Tricarbonyl Complex.
Diastereo-selective
Allylation of the Derived Iron Dienal Paley,
R.S., Rubio, M.B.,
Fernández de la Pradilla, R.,
Dorado, R., Hundal, G. and Martínez-Ripoll, M. Organometallics
(1996) 15, 4672-4674 (doi:10.1021/om960654+)
Synthesis and structural study of [{Pd(C6H4CH2N(CH3)2)}2(μ-Br)(μ-X)]
complexes (X = hydroxide, amide or thiolate)
Ruiz, J., Cutillas, N., Sampedro, J., López, G., Hermoso,
J.A. and Martínez-Ripoll, M. Journal
of Organometallic Chemistry (1996) 526, 67-72 (doi:10.1016/S0022-328X(96)06520-5)
An 18-chloro-4α-hydroxy derivative of 19-acetylteupolin
IV: A neo-clerodane diterpenoid of biological interest
Hundal, G. and Martinez-Ripoll, M. Acta
Crystallographica (1996) C52, 3157-3159 (doi:10.1107/S0108270196009110)
Influence of protonation on crystal packing and thermal behaviour of
tert-butylammonium decavanadates
Wery, A.S.J., Gutierrez-Zorrilla, J.M., Luque, A., Roman, P. and
Martinez-Ripoll, M. Polyhedron
(1996) 15, 4555-4564
(doi:10.1016/0277-5387(96)00186-6) 1993-1995
Cristalografía Cano,
F.H.,
Foces-Foces, C. and Martínez-Ripoll, M. (eds.) Serie:
Nuevas
Tendencias (1995). CSIC (link
to the book)
Macromoléculas
biológicas: Estructura tridimensional Martínez-Ripoll,
M., Romero, A. and Sanz-Aparicio, J. Investigación y Ciencia (1995)
222, 83-84 (link
to the journal)
A New
Approach to Structure Resolution by Molecular Dynamics: Variable
Force Constants Strategy D.
Tranqui, J.A. Hermoso,
M. Averbuch ,G. Tamagnan, G. Leclerc.and M.
Cussac Acta
Crystallographica (1995) B51, 76-80
(doi:10.1107/S010876819400827X) Crystallizacion
and Preliminary X-ray Diffraction
Analysis of a Type I β-Glucosidase
Encoded by the bglA Gene of Bacillus
polymyxa Sanz-Aparicio,
J., Romero, A., Martínez-Ripoll, M.,
Madarro, A., Flors, A. and Polaina, J. Journal
of Molecular Biology (1994) 240, 267-270
(doi:10.1006/jmbi.1994.1441) The Use of
Protein
Homologues in the Rotation Function Aguilar, C.F., Newman,
M.P., Sanz-Aparicio, J., Cooper, J.B., Tickle, I.J. and Blundell, T.L. Acta Crystallographica (1993) A49,
306-315(doi:10.1107/S010876739200847X)
Facilities
The department is very well equipped with a wide range of
crystallographic and computing equipment, wet laboratories
and dedicated facilities for protein production, protein
characterisation,
protein crystallization, X-ray diffraction and computing. A
summary of all these facilities can be obtained through this link.
Protein
production and Molecular Biology
Laboratories
Laboratory
for protein
production
Molecular
Biology Lab
Crystallization
Laboratory
Crystallization
Lab
Crystallization
platform
Crystallization
robot
Crystal-Farm
X-ray
Diffraction Laboratory
Kappa goniometer with CCD
and
rotating anode with mirrors
Imaging plate mounted on a
rotating anode with mirrors
Kappa goniometer with
Photon
detector and X-ray micro-source
Computing
Laboratory
Graphic computers with
stereoview
In
addition, there are several low and high pressure
chromatography systems and a large number of different chromatographic
matrices for optimal protein purification. Analytical techniques,
including electrophoresis are also available in the lab, which combined
with access to mass spectrometry and analytical ultracentrifugation are
used to assess the quality of the proteins produced. In addition,
through our links with remaining groups of the Institute, we
have access
to isothermal titration calorimetry, Nuclear
Magnetic Resonance, analytical ultracentrifugation and MALDI-TOF mass
spectrometry.
Biochemical studies and assays can be
performed using a number of techniques, such as UV-VIS and
CD-spectroscopies, microcalorimetry and fluorimetry.
The ultimate goal is to provide a
regular supply of crystals for X-ray crystallographic structure
determination. Success in this aim is aided by the
presence of a number of temperature-controlled incubators,
high-throughput facilities and dedicated crystallisation rooms.
