Publications
José Manuel Martín García


Serial macromolecular crystallography at ALBA Synchrotron Light Source
J. M. Martin-Garcia, S. Botha, H. Hu, R. Jernigan, A. Castellví, S. Lisova, F. Gil, B. Calisto, I. Crespo, S. Roy-Chowdhury, A. Grieco, G. Ketawala, U. Weierstall, J. Spence, P. Fromme, N. Zatsepin, D. R. Boer and X. Carpena
Journal of Synchrotron Radiation (2022) 29  (doi:10.1107/S1600577522002508)

Co-flow injection for serial crystallography at X-ray free-electron lasers
D. Doppler, M. T. Rabbani, R. Letrun, J. Cruz Villarreal, D. H. Kim, S. Gandhi, A. Egatz-Gomez, M. Sonker, J. Chen, F. H. M. Koua, J. Yang, M. Youssef, V. Mazalova, S. Bajt, M. L. Shelby, M. A. Coleman, M. O. Wiedorn, J. Knoska, S. Schön, T. Sato, M. S. Hunter, A. Hosseinizadeh, C. Kuptiz, R. Nazari, R. C. Alvarez, K. Karpos, S. Zaare, Z. Dobson, E. Discianno, S. Zhang, J. D. Zook, J. Bielecki, R. de Wijn, A. R. Round, P. Vagovic, M. Kloos, M. Vakili, G. K. Ketawala, N. E. Stander, T. L. Olson, K. Morin, J. Mondal, J. Nguyen, J. D. Meza-Aguilar, G. Kodis, S. Vaiana, J. M. Martin-Garcia, V. Mariani, P. Schwander, M. Schmidt, M. Messerschmidt, A. Ourmazd, N. Zatsepin, U. Weierstall, B. D. Bruce, A. P. Mancuso, T. Grant, A. Barty, H. N. Chapman, M. Frank, R. Fromme, J. C. H. Spence, S. Botha, P. Fromme, R. A. Kirian and A. Ros
Journal of Applied Crystallography (2022) 55  (doi: org/10.1107/S1600576721011079)

Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography
Suraj Pandey, George Calvey, Andrea M. Katz, Tek Narsingh Malla, Faisal H. M. Koua, Jose M. Martin-Garcia, Ishwor Poudyal, Jay-How Yang, Mohammad Vakili, Oleksandr Yefanov, Kara A. Zielinski, Sasa Bajt, Salah Awel, Katarina Doerner, Matthias Frank, Luca Gelisio, Rebecca Jernigan, Henry Kirkwood, Marco Kloos, Jayanath Koliyadu, Valerio Mariani, Mitchell D. Miller, Grant Mills, Garrett Nelson, Jose L. Olmos Jr, Alireza Sadri, Tokushi Sato, Alexandra Tolstikova, Weijun Xu, Abbas Ourmazd, John C. H. Spence, Peter Schwander, Anton Barty, Henry N. Chapman, Petra Fromme, Adrian P. Mancuso, George N. Phillips Jr, Richard Bean, Lois Pollack and Marius Schmidt
IUCrJ (2021) 8(6), published on line
(doi: 10.1107/S2052252521008125)

Protein Dynamics and Time Resolved Protein Crystallography at Synchrotron Radiation Sources: Past, Present and Future.
Martin-Garcia, J.M.
Crystals (2021) 11, 521  (doi: 10.3390/cryst11050521

Structural insights into the function of the catalytically active human Taspase1
Nirupa Nagaratnam, Silvia L. Delker, Rebecca Jernigan, Thomas E. Edwards, Janey Snider, Darren Thifault, Dewight Williams, Brent L. Nannenga, Mary Stofega, Lidia Sambucetti, James J. Hsieh, Andrew J. Flint, Petra Fromme, Jose M. Martin-Garcia
Structure (2021) 29, 1–13  (doi: 10.1016/j.str.2021.03.008)

Methods for Crystallization and Structural Determination of M-T7 Protein from Myxoma Virus

Gisriel C, Fromme P, Martin-Garcia JM
Methods Mol Biol. (2021) 2225, 125-162  (doi: 10.1007/978-1-0716-1012-1_8)

Segmented flow generator for serial crystallography at the European X-ray free electron laser
Echelmeier A, Cruz Villarreal J, Messerschmidt M, Kim D, Coe JD, Thifault D, Botha S, Egatz-Gomez A, Gandhi S, Brehm G, Conrad CE, Hansen DT, Madsen C, Bajt S, Meza-Aguilar JD, Oberthür D, Wiedorn MO, Fleckenstein H, Mendez D, Knoška J, Martin-Garcia JM, Hu H, Lisova S, Allahgholi A, Gevorkov Y, Ayyer K, Aplin S, Ginn HM, Graafsma H, Morgan AJ, Greiffenberg D, Klujev A, Laurus T, Poehlsen J, Trunk U, Mezza D, Schmidt B, Kuhn M, Fromme R, Sztuk-Dambietz J, Raab N, Hauf S, Silenzi A, Michelat T, Xu C, Danilevski C, Parenti A, Mekinda L, Weinhausen B, Mills G, Vagovic P, Kim Y, Kirkwood H, Bean R, Bielecki J, Stern S, Giewekemeyer K, Round AR, Schulz J, Dörner K, Grant TD, Mariani V, Barty A, Mancuso AP, Weierstall U, Spence JCH, Chapman HN, Zatsepin N, Fromme P, Kirian RA, Ros A
Nat Commun. (2020) Sep 9; 11(1):4511  (doi: 10.1038/s41467-020-18156-7)

Construction of gateway-compatible baculovirus expression vectors for high- throughput protein expression and in vivo microcrystal screening
Tang Y, Saul J, Nagaratnam N, Martin-Garcia JM, Fromme P, Qiu J, LaBaer J
Sci Rep. (2020) Aug 7;10(1):13323  (doi: 10.1038/s41598-020-70163-2)

Enhanced X-ray diffraction of in vivo-grown μNS crystals by viscous jets at XFELs
Nagaratnam N, Tang Y, Botha S, Saul J, Li C, Hu H, Zaare S, Hunter M, Lowry D, Weierstall U, Zatsepin N, Spence JCH, Qiu J, LaBaer J, Fromme P, Martin- Garcia JM
Acta Crystallogr F Struct Biol Commun. (2020) Jun 1;76(Pt 6):278-289
 (doi: 10.1107/S2053230X20006172)


Serial Femtosecond Crystallography: A Decade at the Forefront in Structural Biology
Fromme, P., Graves, W.S. and Martin‐Garcia, J.M.
In eLS, John Wiley & Sons, Ltd (Ed.). (2020) May 29; 1-17   (doi:10.1002/9780470015902.a0028964)

In cellulo crystallization of Trypanosoma brucei IMP dehydrogenase enables the identification of genuine co-factors
Nass K, Redecke L, Perbandt M, Yefanov O, Klinge M, Koopmann R, Stellato F, Gabdulkhakov A, Schönherr R, Rehders D, Lahey-Rudolph JM, Aquila A, Barty A, Basu S, Doak RB, Duden R, Frank M, Fromme R, Kassemeyer S, Katona G, Kirian R, Liu H, Majoul I, Martin-Garcia JM, Messerschmidt M, Shoeman RL, Weierstall U, Westenhoff S, White TA, Williams GJ, Yoon CH, Zatsepin N, Fromme P, Duszenko M, Chapman HN, Betzel C
Nat Commun. (2020) Jan 30;11(1):620  (doi: 10.1038/s41467-020-14484-w)

Membrane protein megahertz crystallography at the European XFEL
Gisriel C, Coe J, Letrun R, Yefanov OM, Luna-Chavez C, Stander NE, Lisova S, Mariani V, Kuhn M, Aplin S, Grant TD, Dörner K, Sato T, Echelmeier A, Cruz Villarreal J, Hunter MS, Wiedorn MO, Knoska J, Mazalova V, Roy-Chowdhury S, Yang JH, Jones A, Bean R, Bielecki J, Kim Y, Mills G, Weinhausen B, Meza JD, Al- Qudami N, Bajt S, Brehm G, Botha S, Boukhelef D, Brockhauser S, Bruce BD, Coleman MA, Danilevski C, Discianno E, Dobson Z, Fangohr H, Martin-Garcia JM, Gevorkov Y, Hauf S, Hosseinizadeh A, Januschek F, Ketawala GK, Kupitz C, Maia L, Manetti M, Messerschmidt M, Michelat T, Mondal J, Ourmazd A, Previtali G, Sarrou I, Schön S, Schwander P, Shelby ML, Silenzi A, Sztuk-Dambietz J, Szuba J, Turcato M, White TA, Wrona K, Xu C, Abdellatif MH, Zook JD, Spence JCH, Chapman HN, Barty A, Kirian RA, Frank M, Ros A, Schmidt M, Fromme R, Mancuso AP, Fromme P, Zatsepin NA
Nat Commun. (2019) Nov 4;10(1):5021  (doi: 10.1038/s41467-019-12955-3)

High- viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source
Martin-Garcia JM, Zhu L, Mendez D, Lee MY, Chun E, Li C, Hu H, Subramanian G, Kissick D, Ogata C, Henning R, Ishchenko A, Dobson Z, Zhang S, Weierstall U, Spence JCH, Fromme P, Zatsepin NA, Fischetti RF, Cherezov V, Liu W
IUCrJ. (2019) Apr 5;6(Pt 3):412-425  (doi: 10.1107/S205225251900263X)

X-ray Emission Spectroscopy at X-ray Free Electron Lasers: Limits to Observation of the Classical Spectroscopic Response for Electronic Structure Analysis
Jensen SC, Sullivan B, Hartzler DA, Aguilar JM, Awel S, Bajt S, Basu S, Bean R, Chapman HN, Conrad C, Frank M, Fromme R, Martin-Garcia JM, Grant TD, Heymann M, Hunter MS, Ketawala G, Kirian RA, Knoska J, Kupitz C, Li X, Liang M, Lisova S, Mariani V, Mazalova V, Messerschmidt M, Moran M, Nelson G, Oberthür D, Schaffer A, Sierra RG, Vaughn N, Weierstall U, Wiedorn MO, Xavier PL, Yang JH, Yefanov O, Zatsepin NA, Aquila A, Fromme P, Boutet S, Seidler GT, Pushkar Y
J Phys Chem Lett. (2019) Feb 7;10(3):441-446  (doi: 10.1021/acs.jpclett.8b03595)

Solving protein structure from sparse serial microcrystal diffraction data at a storage-ring synchrotron source
Lan TY, Wierman JL, Tate MW, Philipp HT, Martin-Garcia JM, Zhu L, Kissick D, Fromme P, Fischetti RF, Liu W, Elser V, Gruner SM
IUCrJ. (2018) Jul 20;5(Pt 5):548-558  (doi: 10.1107/S205225251800903X)

Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography
Olmos JL Jr, Pandey S, Martin-Garcia JM, Calvey G, Katz A, Knoska J, Kupitz C, Hunter MS, Liang M, Oberthuer D, Yefanov O, Wiedorn M, Heyman M, Holl M, Pande K, Barty A, Miller MD, Stern S, Roy-Chowdhury S, Coe J, Nagaratnam N, Zook J, Verburgt J, Norwood T, Poudyal I, Xu D, Koglin J, Seaberg MH, Zhao Y, Bajt S, Grant T, Mariani V, Nelson G, Subramanian G, Bae E, Fromme R, Fung R, Schwander P, Frank M, White TA, Weierstall U, Zatsepin N, Spence J, Fromme P, Chapman HN, Pollack L, Tremblay L, Ourmazd A, Phillips GN Jr, Schmidt M
BMC Biol. (2018) May 31;16(1):59  (doi: 10.1186/s12915-018-0524-5)

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation
Martin-Garcia JM, Conrad CE, Nelson G, Stander N, Zatsepin NA, Zook J, Zhu L, Geiger J, Chun E, Kissick D, Hilgart MC, Ogata C, Ishchenko A, Nagaratnam N, Roy-Chowdhury S, Coe J, Subramanian G, Schaffer A, James D, Ketwala G, Venugopalan N, Xu S, Corcoran S, Ferguson D, Weierstall U, Spence JCH, Cherezov V, Fromme P, Fischetti RF, Liu W 
IUCrJ. (2017) May 24;4(Pt 4):439-454  (doi: 10.1107/S205225251700570X)

Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide
Deb A, Johnson WA, Kline AP, Scott BJ, Meador LR, Srinivas D, Martin-Garcia JM, Dörner K, Borges CR, Misra R, Hogue BG, Fromme P, Mor TS
PLoS One (2017) Feb 22;12(2):e0172529  (doi: 10.1371/journal.pone.0172529)

Structural enzymology using X-ray free electron lasers
Kupitz C, Olmos JL Jr, Holl M, Tremblay L, Pande K, Pandey S, Oberthür D, Hunter M, Liang M, Aquila A, Tenboer J, Calvey G, Katz A, Chen Y, Wiedorn MO, Knoska J, Meents A, Majriani V, Norwood T, Poudyal I, Grant T, Miller MD, Xu W, Tolstikova A, Morgan A, Metz M, Martin-Garcia JM, Zook JD, Roy-Chowdhury S, Coe J, Nagaratnam N, Meza D, Fromme R, Basu S, Frank M, White T, Barty A, Bajt S, Yefanov O, Chapman HN, Zatsepin N, Nelson G, Weierstall U, Spence J, Schwander P, Pollack L, Fromme P, Ourmazd A, Phillips GN Jr, Schmidt M
Struct Dyn. (2016) Dec 15;4(4):044003   (doi: 10.1063/1.4972069)

The role of water molecules in the binding of class I and II peptides to the SH3 domain of the Fyn tyrosine kinase
Camara-Artigas A, Ortiz-Salmeron E, Andujar-Sánchez M, Bacarizo J, Martin- Garcia JM
Acta Crystallogr F Struct Biol Commun. (2016) Sep;72(Pt 9):707-12  (doi: 10.1107/S2053230X16012310)

Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization
Dörner K, Martin-Garcia JM, Kupitz C, Gong Z, Mallet TC, Chen L, Wachter RM, Fromme P
Cryst Growth Des. (2016) Jul 6;16(7):3838-3845  (doi: 10.1021/acs.cgd.6b00384)

Serial femtosecond crystallography: A revolution in structural biology
Martin-Garcia JM, Conrad CE, Coe J, Roy-Chowdhury S, Fromme P
Arch Biochem Biophys. (2016) Jul 15; 602:32-47  (doi: 10.1016/j.abb.2016.03.036)

Biophysical Characterization of a Vaccine Candidate against HIV-1: The Transmembrane and Membrane Proximal Domains of HIV-1 gp41 as a Maltose Binding Protein Fusion
Gong Z, Martin-Garcia JM, Daskalova SM, Craciunescu FM, Song L, Dörner K, Hansen DT, Yang JH, LaBaer J, Hogue BG, Mor TS, Fromme P
PLoS One  (2015) Aug 21;10(8): e0136507  (doi: 10.1371/journal.pone.0136507)

Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum
Yang JH, Sarrou I, Martin-Garcia JM, Zhang S, Redding KE, Fromme P
Protein Expr Purif. (2015) Oct; 114:1-8  (doi: 10.1016/j.pep.2015.05.006)

Electrostatic effects in the folding of the SH3 domain of the c-Src tyrosine kinase: pH-dependence in 3D-domain swapping and amyloid formation
Bacarizo J, Martinez-Rodriguez S, Martin-Garcia JM, Andujar-Sanchez M, Ortiz-Salmeron E, Neira JL, Camara-Artigas A
PLoS One (2014) Dec 9;9(12): e113224  (doi: 10.1371/journal.pone.0113224)

Purification and biophysical characterization of the CapA membrane protein FTT0807 from Francisella tularensis
Martin-Garcia JM, Hansen DT, Zook J, Loskutov AV, Robida MD, Craciunescu FM, Sykes KF, Wachter RM, Fromme P, Allen JP
Biochemistry (2014) Apr 1;53(12):1958-70  (doi: 10.1021/bi401644s)

3D domain swapping in a chimeric c-Src SH3 domain takes place through two hinge loops
Cámara-Artigas A, Martínez-Rodríguez S, Ortiz-Salmerón E, Martín-García JM
J Struct Biol. (2014) Apr;186(1):195-203  (doi: 10.1016/j.jsb.2014.02.007)

Proliferating cell nuclear antigen (PCNA) interactions in solution studied by NMR
De Biasio A, Campos-Olivas R, Sánchez R, López-Alonso JP, Pantoja-Uceda D, Merino N, Villate M, Martin-Garcia JM, Castillo F, Luque I, Blanco FJ
PLoS One (2012) 7(11): e48390  (doi: 10.1371/journal.pone.0048390)

The promiscuous binding of the Fyn SH3 domain to a peptide from the NS5A protein
Martin-Garcia JM, Luque I, Ruiz-Sanz J, Camara-Artigas A
Acta Crystallogr D Biol Crystallogr. (2012) Aug;68(Pt 8):1030-40  (doi: 10.1107/S0907444912019798)

pH-dependent structural conformations of B-phycoerythrin from Porphyridium cruentum
Camara-Artigas A, Bacarizo J, Andujar-Sanchez M, Ortiz-Salmeron E, Mesa- Valle C, Cuadri C, Martin-Garcia JM, Martinez-Rodriguez S, Mazzuca-Sobczuk T, Ibañez MJ, Allen JP
FEBS J. (2012) Oct;279(19):3680-3691  (doi: 10.1111/j.1742-4658.2012.08730.x)

Interfacial water molecules in SH3 interactions: a revised paradigm for polyproline recognition
Martin-Garcia JM, Ruiz-Sanz J, Luque I
Biochem J. (2012) Mar 1;442(2):443-51  (doi: 10.1042/BJ20111089)

High-resolution structure of an alpha-spectrin SH3-domain mutant with a redesigned hydrophobic core
Cámara-Artigas A, Andújar-Sánchez M, Ortiz-Salmerón E, Cuadri C, Cobos ES, Martin-Garcia JM
Acta Crystallogr Sect F Struct Biol Cryst Commun. (2010) Sep 1;66(Pt 9):1023-7  (doi: 10.1107/S1744309110030095)

Intertwined dimeric structure for the SH3 domain of the c-Src tyrosine kinase induced by polyethylene glycol binding
Cámara-Artigas A, Martín-García JM, Morel B, Ruiz-Sanz J, Luque I
FEBS Lett. (2009) Feb 18;583(4):749-53  (doi: 10.1016/j.febslet.2009.01.036)

Molecular basis of histone H3K4me3 recognition by ING4
Palacios A, Muñoz IG, Pantoja-Uceda D, Marcaida MJ, Torres D, Martín-García JM, Luque I, Montoya G, Blanco FJ
 J Biol Chem. (2008) Jun 6;283(23):15956-64  (doi: 10.1074/jbc.M710020200)

Crystallographic structure of the SH3 domain of the human c-Yes tyrosine kinase: loop flexibility and amyloid aggregation
Martín-García JM, Luque I, Mateo PL, Ruiz-Sanz J, Cámara-Artigas A. .
FEBS Lett. (2007) May 1;581(9):1701-6  (doi: 10.1016/j.febslet.2007.03.059)