Publications
Beatriz González Pérez
Substrate promiscuity of inositol 1,4,5-trisphosphate kinase driven by structurally-modified ligands and active site plasticity
María Ángeles Márquez-Moñino, Raquel
Ortega-García, Hayley Whitfield, Andrew M. Riley, Lourdes
Infantes, Shane W. Garrett, Megan L. Shipton, Charles A. Brearley,
Barry V. L. Potter & Beatriz González
Nature Communications (2024) 15, 1502 (doi: 10.1038/s41467-024-45917-5)
Insights into the Structure of the Highly Glycosylated Ffase from Rhodotorula dairenensis Enhance Its Biotechnological Potential
E.
Jiménez-Ortega, E. Narmontaite, B. González-Pérez,
Francisco J. Plou, M Fernández-Lobato, J Sanz-Aparicio
International Journal of Molecular Sciences (2022) 23, 14981 (doi: 10.3390/ijms232314981)
Multiple
substrate recognition by yeast diadenosine and diphosphoinositol
polyphosphate phosphohydrolase through phosphate clamping
María
Ángeles Márquez-Moñino, Raquel Ortega-García, Megan L. Shipton, Elsa
Franco-Echevarría, Andrew M. Riley, Julia Sanz-Aparicio, Barry V.L.
Potter and Beatriz González
Science Advances (2021) 7, eabf6744 (doi: 10.1126/sciadv.abf6744)
Noncatalytic aspartate at the exonuclease
domain of proofreading DNA polymerases regulates both degradative and
synthetic activities
Del Prado A, Franco-Echevarría E, González B,
Blanco L, Salas M, de Vega M.
Proceedings
of the National Academy of Sciences, PNAS (2018) 115, E2921-E2929
(doi:10.1073/pnas.1718787115)
Crystallization and Preliminary X-Ray Diffraction Analysis of a Mammal Inositol 1, 3, 4, 5, 6-Pentakisphosphate 2-Kinase
Franco-Echevarría E., Sanz-Aparicio J., Troffer-Charlier N., Poterszman A., Gonzalez, B
The Protein Journal (2017) 36, 240–248 (doi: 10.1007/s10930-017-9717-y)
The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition
Franco-Echevarría
E, González-Polo N, Zorrilla S, Martínez-Lumbreras S, Santiveri CM,
Campos-Olivas R, Sánchez M, Calvo O, González B and Pérez-Cañadillas
JM
Nucleic Acids Research (2017) 45, 10293-10305 (doi:10.1093/nar/gkx685)
The
crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate
2-kinase reveals a new zinc-binding site and key features for protein
function
Franco-Echevarría E, Sanz-Aparicio J, Brearley CA, González-Rubio JM, González B.
Journal of Biological Chemistry (2017) 292, 10534-10548 (doi:10.1074/jbc.M117.780395)
Structural analysis of β-fructofuranosidase from Xanthophyllomyces dendrorhous reveals unique features and the crucial role of N-glycosylation in oligomerization and activity
M Ramírez-Escudero, M Gimeno-Pérez, B González, D
Linde, Z Merzdo, M Fernández-Lobato, J Sanz-Aparicio
Journal of
Biological Chemistry (2016) 291, 6843-6857 (doi:10.1074/jbc.M115.708495)
Exploring
multimodularity in plant cell-wall deconstruction: Structural and
functional analysis of the CBM22-1-CBM22-2 tandem from bacterial Xyn10C
Sainz-Polo MA, González B, Menendez, M, Pastor J. and Sanz-Aparicio J.
Journal of
Biological Chemistry (2015) 290, 17116-17130 (doi: 10.1074/jbc.m115.659300)
Crystallization
and preliminary X-ray diffraction analysis of the N-terminal domain of
Paenibacillus barcinonensis xylanase 10C containing the CBM22-1-CBM22-2
tandem
Sainz-Polo MA, González B, Pastor J, Sanz-Aparicio J
Acta Crystallographica (2015) F71, 136-140 (doi: 10.1107/S2053230X14027496)
The new InsP3Kinase inhibitor BIP4 is competitive to InsP3 and blocks proliferation and adhesion of lung cancer cells
Schröder D, Tödter K, Franco-Echevarría, E, Gonzalez B, Rohaly G and Sabine Windhorst
Biochemical Pharmacology (2015) 96, 143-150 (doi: 10.1016/j.bcp.2015.05.004)
Structural
analysis of glucuronoxylan specific Xyn30D and its attached CBM35
domain give insights into the role of modularity in specificity
MA Sainz-Polo, SV Valenzuela, B González, FIJ Pastor, J Sanz-Aparicio
Journal of
Biological Chemistry (2014) 289, 31088-31101 (doi: 10.1074/jbc.M114.597732)
A new calmodulin binding motif for inositol 1,4,5-trisphosphate 3-kinase regulation
Franco-Echevarria, E., Baños-Sanz, J.I., Monterroso, B., Round A, Sanz-Aparicio, J., González, B.
Biochemical Journal (2014) 463, 319-328 (doi:10.1042/BJ20140757)
Crystal structure and functional insights into uracil-DNA glycosylase inhibition by phage ϕ29 DNA mimic protein p56
José Ignacio Baños-Sanz, Laura
Mojardín, Julia Sanz-Aparicio, José M. Lázaro,
Laurentino Villar, Gemma Serrano-Heras, Beatriz González, and
Margarita Salas
Nucleic Acids Research (2013) 41, 6761-6773 (doi:10.1093/nar/gkt395)
The three-dimensional structure of Saccharomyces
invertase: role of a noncatalytic domain in oligomerization and
substrate specificity
M. Angela Sainz-Polo, Mercedes Ramírez-Escudero, Alvaro
Lafraya, Beatriz González, Julia Marín-Navarro,
Julio Polaina and Julia Sanz-Aparicio
Journal
of Biological Chemistry (2013) 288, 9755-9766 (doi:10.1074/jbc.M112.446435)
NADP+ Binding to the Regulatory Subunit of
Methionine Adenosyltransferase II Increases Intersubunit Binding
Affinity in the Hetero-Trimer
Gonzalez,
B; Garrido, F; Ortega, R; Martínez-Julvez, M; Revilla-Guarinos, A;
Pérez-Pertejos. Y; Velázquez-Campoy, A; Sanz-Aparicio, J; and Pajares, M
PLoS ONE (2012) 7(11), e50329 (doi:10.1371/journal.pone.0050329)
Conformational
Changes Undergone by Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase Upon
Substrate Binding: The Role of N-lobe and Enantiomeric Substrate
Preference
José Ignacio Baños-Sanz, Julia Sanz-Aparicio, Hayley
Whitfield, Chris Hamilton, Charles A. Brearley and Beatriz
González
Journal of Biological Chemistry (2012) 287, 29237-29249 (doi:10.1074/jbc.M112.363671)
Expression, purification, crystallization and
preliminary X-ray diffraction analysis of the apo form of InsP(5) 2-K
from Arabidopsis thaliana
J.I. Baños-Sanz, J. Sanz-Aparicio, C.A. Brearley and B. González
Acta Crystallographica (2012) F68, 701-704 (doi:10.1107/S1744309112017307)
Structural and kinetic insights reveal that the amino acid pair GLN228/ASN254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics
Miguel
Alvaro-Benito, M. Angela Sainz-Polo, David Gonzalez-Perez, Beatriz
Gonzalez, Francisco J. Plou, Maria Fernandez-Lobato, and Julia Sanz
Aparicio
Journal of Biological Chemistry (2012) 287, 19674-19686 (doi:10.1074/jbc.M112.355503)
Structural
and kinetic analysis of Schwanniomyces Occidentalis invertase reveals a
new oligomerization pattern and the role of its supplementary domain
in substrate binding
Alvaro-Benito M., Polo A., González B., Fernandez-Lobato M. & Sanz-Aparicio J.
Journal of
Biological Chemistry (2010) 285, 13930-13941 (doi: 10.1074/jbc.M109.095430)
Structural
Insights into the Specificity of Xyn10B from Paenibacillus
barcinonensis and Its Improved Stability by Forced Protein Evolution
Gallardo, O., Pastor J., Polaina J., Diaz P., Lysek R., Vogel P., Isorna P., González B. & Sanz-Aparicio J.
Journal of
Biological Chemistry (2010) 285, 2721–2733 (doi: 10.1074/jbc.M109.064394)
Inositol 1,3,4,5,6-pentakisphosphate 2-kinase
is a distant IPK member with a singular inositide binding site for
axial 2-OH recognition
B. González, J. Baños-Sanz, M. Villate, C. Brearley and J. Sanz-Aparicio
Proceedings
of the National Academy of Sciences (2010) 107, 9608-9613 (doi:10.1073/pnas.0912979107)
Crystallization and preliminary X-ray
diffraction analysis of inositol 1,3,4,5,6-pentakisphosphate kinase
from Arabidopsis thaliana
Baños-Sanz, J.I., Villate, M., Sanz-Aparicio, J., Brearley, C.A. and González B.
Acta Crystallographica (2010) F66, 102-106 (doi:10.1107/S1744309109051057)
Targeted
polypharmacology: discovery of dual inhibitors of tyrosine and
phosphoinositide kinases
Apsel
B., Blair J.A., Gonzalez B., Nazif T.M., Feldman M.E., Aizenstein B.,
Hoffman R., Williams R.L., Shokat K.M. and Knight Z.A.
Nature
Chemical Biology (2008) 4, 691-699
Crystal structures of
Paenibacillus polymyxa β-Glucosidase B complexes reveal the
molecular basis of substrate specificity
Isorna P., Polaina J., Latorre-Garcia L., Cañada F.J.
González B. and Sanz-Aparicio J.
Journal Molecular Biology (2007)
371 (5), 1204-1218
A pharmacological map of the
PI3-K family defines a role for p110α in insulin signaling
Knight Z.A., González B., Feldman M.E., Zunder
E.R.,
Goldenberg D.D., Williams O., Loewith R., Stokoe D., Balla A., Toth B.,
Balla T., Weiss W.A., Williams R.L. and Shokat K.M.
Cell (2006) 125, 733-747 [PDF-copy]
ESCRT-II, an endosome-associated
complex
required for protein sorting: crystal structure and interactions with
ESCRT-III and membranes
Teo H., Perisic O., González B. and Williams R.L.
Developmental Cell (2004) 7, 559-569 [PDF-copy]
The structure of human inositol
1,4,5-trisphosphate
3-kinase: The substrate binding reveals why it is not a
phosphoinositide 3-kinase
González B., Schell M.J., Letcher, A.J., Veprintsev, D.B.,
Irvine R. and Williams R.L.
Molecular Cell (2004) 15,689-701
Crystal structure of rat liver betaine
homocysteine
S-methyltransferase reveals new oligomerization features and
conformational changes upon substrate binding
González B., Pajares M.A., Martinez-Ripoll M., Blundell T.
and Sanz-Aparicio J.
Journal Molecular Biology (2004) 338, 771-782
Crystal structure of several
MAT-complexes with substrate analogues and products: Insights into
catalytic mechanism.
González B., Pajares M.A, Hermoso J.A., Guillerm D., Gillerm
G. and Sanz-Aparicio J.
Journal Molecular Biology (2003) 33, 407-416
Active-site mutagenesis study of
rat liver betaine homocysteine S-methyltransferase
González B., Campillo N., Garrido F., Gasset M., Sanz
Aparicio J. and Pajares M.A.
Biochemical J. (2002) 370, 945-952
Crystallization and preliminary X-ray study
of recombinant betaine-homocysteine S-methyltransferase from rat liver
González B., Pajares M.A., Too H.P., Garrido F., Blundell T.
L. and Sanz-Aparicio. J.
Acta Cryst. (2002) D59, 1507-1510
Identification and possible role
of a disulfide bond in rat liver methionine adenosyltransferase
López Vara M.C., Martínez Chantar M.L.,
González
B., Gasset M., Álvarez L., GarridoF., Hermoso J.,
Sanz-Aparicio
J. and Pajares M.A.
V Workshop on Methionine Metabolism: Molecular Mechanisms and Clinical
Implications (2000) 246-252
The crystal structure of tetrameric
methionine
adenosyltranferase from rat liver reveals the methionine-binding site
González B., Pajares M.A., Hermoso J.A.,
Álvarez
L., Garrido F., Sufrin J.R. and Sanz-Aparicio J.
Journal Molecular Biology (2000) 300,
363-375
Directed Evolution of β-Glucosidase A from
Paenibacillus polymyxa to thermal resistance
González-Blasco G., Sanz-Aparicio J., González
B., Hermoso J. A. and Polaina J.
J. Biol. Chem. (2000) 275, 13708-13712
Structural basis of increased
resistance to
thermal denaturation induced by single aminoacide substitution of
b-Glucosidase A from Bacillus polymyxa
Sanz-Aparicio J., Hermoso J., Martínez-Ripoll M,
González B., López Camacho C. and Polaina J.
Proteins (1998) 33, 4, 567-576
Diels-Alder cycloaducts of C60 Fullereno with
pyrimidine o-quinodimethanes
González B., Herrera A., Illescas B.,
Martín N., Martínez R. and Moreno F.
J. Org. Chem. (1998) 63, 20, 6807-6813
Mass spectra of some new heterocycles:
ciclobuta-pyrimidines and their Diels-Alder aducts with C60
Martínez R., Herrera A.,
Martín N., González B. and Illescas B.
Rapid Comunications in Mass Spectrometry (1998) 12,568-570
An expeditous procedure for the
generation of pyrimidine o-quinodimethanes
Herrera A., Martínez R., González B.,
Martín N., Seoane C. and Illescas B.
Tetrahedron Let. (1997) 38, 27, 4873-4876