Beatriz González Pérez

Substrate promiscuity of inositol 1,4,5-trisphosphate kinase driven by structurally-modified ligands and active site plasticity
María Ángeles Márquez-Moñino, Raquel Ortega-García, Hayley Whitfield, Andrew M. Riley, Lourdes Infantes, Shane W. Garrett, Megan L. Shipton, Charles A. Brearley, Barry V. L. Potter & Beatriz González
Nature Communications (2024) 15, 1502   (doi: 10.1038/s41467-024-45917-5)

Insights into the Structure of the Highly Glycosylated Ffase from Rhodotorula dairenensis Enhance Its Biotechnological Potential
E. Jiménez-Ortega, E. Narmontaite, B. González-Pérez, Francisco J. Plou, M Fernández-Lobato, J Sanz-Aparicio
International Journal of Molecular Sciences (2022) 23, 14981  (doi: 10.3390/ijms232314981)

Multiple substrate recognition by yeast diadenosine and diphosphoinositol polyphosphate phosphohydrolase through phosphate clamping
María Ángeles Márquez-Moñino, Raquel Ortega-García, Megan L. Shipton, Elsa Franco-Echevarría, Andrew M. Riley, Julia Sanz-Aparicio, Barry V.L. Potter and Beatriz González
Science Advances (2021) 7, eabf6744  (doi: 10.1126/sciadv.abf6744)

Noncatalytic aspartate at the exonuclease domain of proofreading DNA polymerases regulates both degradative and synthetic activities
Del Prado A, Franco-Echevarría E, González B, Blanco L, Salas M, de Vega M.
Proceedings of the National Academy of Sciences, PNAS (2018) 115, E2921-E2929  (doi:10.1073/pnas.1718787115)

Crystallization and Preliminary X-Ray Diffraction Analysis of a Mammal Inositol 1, 3, 4, 5, 6-Pentakisphosphate 2-Kinase
Franco-Echevarría E., Sanz-Aparicio J., Troffer-Charlier N., Poterszman A., Gonzalez, B
The Protein Journal (2017) 36, 240–248  (doi: 10.1007/s10930-017-9717-y)

The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition
Franco-Echevarría E, González-Polo N, Zorrilla S, Martínez-Lumbreras S, Santiveri CM, Campos-Olivas R, Sánchez M, Calvo O, González B and Pérez-Cañadillas JM
Nucleic Acids Research (2017) 45, 10293-10305  (doi:10.1093/nar/gkx685)

The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function
Franco-Echevarría E, Sanz-Aparicio J, Brearley CA,  González-Rubio JM, González B.
Journal of Biological Chemistry (2017) 292, 10534-10548

Structural analysis of β-fructofuranosidase from Xanthophyllomyces dendrorhous reveals unique features and the crucial role of N-glycosylation in oligomerization and activity
M Ramírez-Escudero, M Gimeno-Pérez, B González, D Linde, Z Merzdo, M Fernández-Lobato, J Sanz-Aparicio
Journal of Biological Chemistry (2016) 291, 6843-6857  (doi:10.1074/jbc.M115.708495)

Exploring multimodularity in plant cell-wall deconstruction: Structural and functional analysis of the CBM22-1-CBM22-2 tandem from bacterial Xyn10C
Sainz-Polo MA, González B, Menendez, M, Pastor J. and  Sanz-Aparicio J.
Journal of Biological Chemistry (2015) 290, 17116-17130  (doi: 10.1074/jbc.m115.659300)

Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Paenibacillus barcinonensis xylanase 10C containing the CBM22-1-CBM22-2 tandem
Sainz-Polo MA, González B, Pastor J, Sanz-Aparicio J
Acta Crystallographica (2015) F71, 136-140   (doi: 10.1107/S2053230X14027496)

The new InsP3Kinase inhibitor BIP4 is competitive to InsP3 and blocks proliferation and adhesion of lung cancer cells
Schröder D, Tödter K, Franco-Echevarría, E, Gonzalez  B, Rohaly G and Sabine Windhorst
Biochemical Pharmacology (2015) 96, 143-150  (doi: 10.1016/j.bcp.2015.05.004)

Structural analysis of glucuronoxylan specific Xyn30D and its attached CBM35 domain give insights into the role of modularity in specificity
MA Sainz-Polo, SV Valenzuela, B González, FIJ Pastor, J Sanz-Aparicio
Journal of Biological Chemistry (2014) 289, 31088-31101  (doi: 10.1074/jbc.M114.597732)

A new calmodulin binding motif for inositol 1,4,5-trisphosphate 3-kinase regulation
Franco-Echevarria, E., Baños-Sanz, J.I., Monterroso, B., Round A, Sanz-Aparicio, J., González, B.
Biochemical Journal (2014) 463, 319-328  (doi:10.1042/BJ20140757)

Crystal structure and functional insights into uracil-DNA glycosylase inhibition by phage ϕ29 DNA mimic protein p56

José Ignacio Baños-Sanz, Laura Mojardín, Julia Sanz-Aparicio, José M. Lázaro, Laurentino Villar, Gemma Serrano-Heras, Beatriz González, and Margarita Salas
Nucleic Acids Research (2013) 41, 6761-6773 (doi:10.1093/nar/gkt395)

The three-dimensional structure of Saccharomyces invertase: role of a noncatalytic domain in oligomerization and substrate specificity
M. Angela Sainz-Polo, Mercedes Ramírez-Escudero, Alvaro Lafraya, Beatriz González, Julia Marín-Navarro, Julio Polaina and Julia Sanz-Aparicio
Journal of Biological Chemistry (2013) 288, 9755-9766  (doi:10.1074/jbc.M112.446435)

NADP+ Binding to the Regulatory Subunit of Methionine Adenosyltransferase II Increases Intersubunit Binding Affinity in the Hetero-Trimer
Gonzalez, B; Garrido, F; Ortega, R; Martínez-Julvez, M; Revilla-Guarinos, A; Pérez-Pertejos. Y; Velázquez-Campoy, A; Sanz-Aparicio, J; and Pajares, M
PLoS ONE (2012) 7(11), e50329  

Conformational Changes Undergone by Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase Upon Substrate Binding: The Role of N-lobe and Enantiomeric Substrate Preference
José Ignacio Baños-Sanz, Julia Sanz-Aparicio, Hayley Whitfield, Chris Hamilton, Charles A. Brearley and Beatriz González
Journal of Biological Chemistry (2012) 287, 29237-29249  (doi:10.1074/jbc.M112.363671)

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the apo form of InsP(5) 2-K from Arabidopsis thaliana
J.I. Baños-Sanz, J. Sanz-Aparicio, C.A. Brearley and  B. González
Acta Crystallographica (2012) F68, 701-704  (doi:10.1107/S1744309112017307)

Structural and kinetic insights reveal that the amino acid pair GLN228/ASN254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics
Miguel Alvaro-Benito, M. Angela Sainz-Polo, David Gonzalez-Perez, Beatriz Gonzalez, Francisco J. Plou, Maria Fernandez-Lobato, and Julia Sanz Aparicio
Journal of Biological Chemistry (2012) 287, 19674-19686  (doi:10.1074/jbc.M112.355503)

Structural and kinetic analysis of Schwanniomyces Occidentalis invertase reveals a new oligomerization pattern  and the role of its supplementary  domain in substrate binding
Alvaro-Benito M., Polo A., González B., Fernandez-Lobato M. & Sanz-Aparicio J.
Journal of Biological Chemistry (2010) 285, 13930-13941  (doi: 10.1074/jbc.M109.095430)

Structural Insights into the Specificity of Xyn10B from Paenibacillus barcinonensis and Its Improved Stability by Forced Protein Evolution
Gallardo, O., Pastor J., Polaina J., Diaz P., Lysek R., Vogel P., Isorna P., González B. & Sanz-Aparicio J.
Journal of Biological Chemistry (2010) 285, 2721–2733  (doi: 10.1074/jbc.M109.064394)

Inositol 1,3,4,5,6-pentakisphosphate 2-kinase is a distant IPK member with a singular inositide binding site for axial 2-OH recognition

B. González, J. Baños-Sanz, M. Villate, C. Brearley and J. Sanz-Aparicio
Proceedings of the National Academy of Sciences (2010) 107, 9608-9613  (doi:10.1073/pnas.0912979107)

Crystallization and preliminary X-ray diffraction analysis of inositol 1,3,4,5,6-pentakisphosphate kinase from Arabidopsis thaliana
Baños-Sanz, J.I., Villate, M., Sanz-Aparicio, J., Brearley, C.A. and González B.
Acta Crystallographica (2010) F66, 102-106  (doi:10.1107/S1744309109051057)

Targeted polypharmacology: discovery of dual inhibitors of tyrosine and phosphoinositide kinases
Apsel B., Blair J.A., Gonzalez B., Nazif T.M., Feldman M.E., Aizenstein B., Hoffman R., Williams R.L., Shokat K.M. and Knight Z.A.
Nature Chemical Biology (2008) 4, 691-699

Crystal structures of Paenibacillus polymyxa β-Glucosidase B complexes reveal the molecular basis of substrate specificity
Isorna P., Polaina J., Latorre-Garcia L., Cañada F.J. González B. and Sanz-Aparicio J.
Journal Molecular Biology (2007) 371 (5), 1204-1218

A pharmacological map of the PI3-K family defines a role for p110α in insulin signaling
Knight Z.A., González B.,  Feldman M.E., Zunder E.R., Goldenberg D.D., Williams O., Loewith R., Stokoe D., Balla A., Toth B., Balla T.,  Weiss W.A., Williams R.L. and Shokat K.M.
Cell (2006) 125, 733-747    

ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes
Teo H., Perisic O., González B. and Williams R.L.
Developmental Cell (2004) 7, 559-569   

The structure of human inositol 1,4,5-trisphosphate 3-kinase: The substrate binding reveals why it is not a phosphoinositide 3-kinase
González B., Schell M.J., Letcher, A.J., Veprintsev, D.B., Irvine R. and Williams R.L.
Molecular Cell (2004) 15,689-701           

Crystal structure of rat liver betaine homocysteine S-methyltransferase reveals new oligomerization features and conformational changes upon substrate binding
González B., Pajares M.A., Martinez-Ripoll M., Blundell T. and Sanz-Aparicio J.
Journal Molecular Biology (2004) 338, 771-782

Crystal structure of several MAT-complexes with substrate analogues and products: Insights into catalytic mechanism.
González B., Pajares M.A, Hermoso J.A., Guillerm D., Gillerm G. and Sanz-Aparicio J.
Journal Molecular Biology (2003) 33, 407-416

Active-site mutagenesis study of rat liver betaine homocysteine S-methyltransferase
González B., Campillo N., Garrido F., Gasset M., Sanz Aparicio J. and Pajares M.A.
Biochemical J. (2002) 370, 945-952    

Crystallization and preliminary X-ray study of recombinant betaine-homocysteine S-methyltransferase from rat liver
González B., Pajares M.A., Too H.P., Garrido F., Blundell T. L. and Sanz-Aparicio. J.
Acta Cryst. (2002) D59, 1507-1510

Identification and possible role of a disulfide bond in rat liver methionine adenosyltransferase
López Vara M.C., Martínez Chantar M.L., González B., Gasset M., Álvarez L., GarridoF., Hermoso J., Sanz-Aparicio J. and Pajares M.A.
V Workshop on Methionine Metabolism: Molecular Mechanisms and Clinical Implications (2000) 246-252    

The crystal structure of tetrameric methionine adenosyltranferase from rat liver reveals the methionine-binding site González B.,  Pajares M.A., Hermoso J.A., Álvarez L., Garrido F., Sufrin J.R. and Sanz-Aparicio J.
Journal Molecular Biology (2000) 300, 363-375   

Directed Evolution of β-Glucosidase A from Paenibacillus polymyxa to thermal  resistance
González-Blasco G., Sanz-Aparicio J., González B., Hermoso J. A. and  Polaina J.
J. Biol. Chem. (2000) 275, 13708-13712

Structural basis of increased resistance to thermal denaturation induced by single aminoacide substitution of b-Glucosidase A from Bacillus polymyxa
Sanz-Aparicio J., Hermoso J., Martínez-Ripoll M, González B., López Camacho C. and Polaina J.
Proteins (1998) 33, 4, 567-576    

Diels-Alder cycloaducts of C60 Fullereno with pyrimidine o-quinodimethanes
González B.,  Herrera A., Illescas B., Martín N., Martínez R. and  Moreno F.
J. Org. Chem. (1998) 63, 20, 6807-6813    

Mass spectra of some new heterocycles: ciclobuta-pyrimidines and their Diels-Alder aducts with C60
Martínez R., Herrera A., Martín N., González B. and Illescas B.
Rapid Comunications in Mass Spectrometry (1998) 12,568-570

An expeditous procedure for the generation of pyrimidine o-quinodimethanes
Herrera A., Martínez R., González B., Martín N., Seoane C. and Illescas B.
Tetrahedron Let. (1997) 38, 27, 4873-4876